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The map label for this gene is rstB [H]

Identifier: 17547782

GI number: 17547782

Start: 3301385

End: 3302686

Strand: Reverse

Name: rstB [H]

Synonym: RSc3063

Alternate gene names: 17547782

Gene position: 3302686-3301385 (Counterclockwise)

Preceding gene: 17547783

Following gene: 17547780

Centisome position: 88.87

GC content: 68.05

Gene sequence:

>1302_bases
ATGTTCCGCTCAACGCTGAAGCTATCGCTCATGATGCTGGTGGCCGGCGCGATTGCCGTCCAGGCGCTCAGTTGGTCGGC
CGGCAGGCTGTTCCACGACACCCTGACGCAGGGCCAGCGCGAGGGGCGCAAGGGTTACGTCTTCCTGTTGCGGGAGTATC
TCGATCGCTTTCCCGGCGAGGCTCGCGCCACGGCCATCCAGCGCCTCAACAACAACGCGACAGAGCTGTTCGACATGGTT
GAGCCCGATACGGTCGCCGATCTGAGCACCGAGCAGCGGCGCGACCTAGCCGATGGCAAGCTGGTTGTGATGACCAACCG
CATGGATTACTACCTGCCGTTGCGCGACGGCACCGTGCTGCATGCACGCTTTGAAGACATCGGCTATTACACCGCCATCA
AGATCATCGCCTTCTGCCTCATTGCCATCGCCACGCTGCTGCCGATCCTGTTCTGGAGCTGGCTGCTGTGGCGCGACCTG
CGCGCCCTGGAGGAGGCGGCGCGCGGCTTTGGCGGCGGCATGCTGTCCACGCGCGCGCACCTGCGGCGGGGCTCCAACGT
CCACGCGCTCGCGCGGCAGTTCAACGACATGGCCGAGCGCATCCAGGGCTCGATCCAGCACCAACGCGAGATGATGAACG
GCATCTCGCACGAGCTGAAGACGCCGATCGCGCGGCTGGAGTTCGGCATCGCGCTGTTGCAGTCGCCGGTGCCGGAAGAC
CAGCGCAAGGCCCGCTTCGATGCCCTGCGCAGCGATGTGCGCGAACTCGACGAACTGGTCACGGAGCTGCTGGCGCTGAG
CCGGCTGGAGCAGGGTGCCACTCACCTCGTGCTGATGCGCGTGACGGTCGGCGAATGGCTCGACAGCGTCGTCGCCAACA
TCGCCGACGATGTGGCCGACCGGCAGCTCACGCTCGCCGTCCACGCCGATTTCGCACCGGCGCACCACGTCTGCGATCCG
CGCCTGGTCGCGCGCGCGCTGCTCAACCTGATCCGCAATGCCATGCGCTATGCGCGCCAGGCCATCACCGTGCGCGCCGA
GGCCGGCACCTACGGCAGCCTGTGCCTGACGGTGGAAGACGATGGCCCCGGCATTCCCGCCGCCGAGCGCGCGCGCGTGT
TCGAGCCGTTCTACCGGCTCGACGCCAGCCGCGACCGCCATACCGGCGGCTTCGGCCTGGGCCTGGCGATCGTGCGGCGG
ATCGCGCTGGTGCACGGCGGCGAGGTGCGGCTCGATACGGGCGGCTCGGGCGGCGCCCGCTTTGTCCTGCTGCTGCCGGC
GATGCCGCTGCCGATGCAGTAG

Upstream 100 bases:

>100_bases
CCGCCGCTTCGGCGACGCCTCGCCCGAGCCACACAAGATCAAGACGATCTGGGGGCGCGGCTATTTGTTCAGCCCCTCGG
CGTGGGAGGAGTGACACCGC

Downstream 100 bases:

>100_bases
GCCCGCGGCATCACGCGGCCTGCGTCTCCGGCCCCGGCCGGTGCAGGAACGCGCTCGGGCTCTGGCCGAAGGCGCGCCGG
AACATCGCCGAGAACGCGCT

Product: transmembrane sensor histidine kinase transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MFRSTLKLSLMMLVAGAIAVQALSWSAGRLFHDTLTQGQREGRKGYVFLLREYLDRFPGEARATAIQRLNNNATELFDMV
EPDTVADLSTEQRRDLADGKLVVMTNRMDYYLPLRDGTVLHARFEDIGYYTAIKIIAFCLIAIATLLPILFWSWLLWRDL
RALEEAARGFGGGMLSTRAHLRRGSNVHALARQFNDMAERIQGSIQHQREMMNGISHELKTPIARLEFGIALLQSPVPED
QRKARFDALRSDVRELDELVTELLALSRLEQGATHLVLMRVTVGEWLDSVVANIADDVADRQLTLAVHADFAPAHHVCDP
RLVARALLNLIRNAMRYARQAITVRAEAGTYGSLCLTVEDDGPGIPAAERARVFEPFYRLDASRDRHTGGFGLGLAIVRR
IALVHGGEVRLDTGGSGGARFVLLLPAMPLPMQ

Sequences:

>Translated_433_residues
MFRSTLKLSLMMLVAGAIAVQALSWSAGRLFHDTLTQGQREGRKGYVFLLREYLDRFPGEARATAIQRLNNNATELFDMV
EPDTVADLSTEQRRDLADGKLVVMTNRMDYYLPLRDGTVLHARFEDIGYYTAIKIIAFCLIAIATLLPILFWSWLLWRDL
RALEEAARGFGGGMLSTRAHLRRGSNVHALARQFNDMAERIQGSIQHQREMMNGISHELKTPIARLEFGIALLQSPVPED
QRKARFDALRSDVRELDELVTELLALSRLEQGATHLVLMRVTVGEWLDSVVANIADDVADRQLTLAVHADFAPAHHVCDP
RLVARALLNLIRNAMRYARQAITVRAEAGTYGSLCLTVEDDGPGIPAAERARVFEPFYRLDASRDRHTGGFGLGLAIVRR
IALVHGGEVRLDTGGSGGARFVLLLPAMPLPMQ
>Mature_433_residues
MFRSTLKLSLMMLVAGAIAVQALSWSAGRLFHDTLTQGQREGRKGYVFLLREYLDRFPGEARATAIQRLNNNATELFDMV
EPDTVADLSTEQRRDLADGKLVVMTNRMDYYLPLRDGTVLHARFEDIGYYTAIKIIAFCLIAIATLLPILFWSWLLWRDL
RALEEAARGFGGGMLSTRAHLRRGSNVHALARQFNDMAERIQGSIQHQREMMNGISHELKTPIARLEFGIALLQSPVPED
QRKARFDALRSDVRELDELVTELLALSRLEQGATHLVLMRVTVGEWLDSVVANIADDVADRQLTLAVHADFAPAHHVCDP
RLVARALLNLIRNAMRYARQAITVRAEAGTYGSLCLTVEDDGPGIPAAERARVFEPFYRLDASRDRHTGGFGLGLAIVRR
IALVHGGEVRLDTGGSGGARFVLLLPAMPLPMQ

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=390, Percent_Identity=34.1025641025641, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1790346, Length=267, Percent_Identity=33.7078651685393, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=31.5315315315315, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789808, Length=270, Percent_Identity=29.2592592592593, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786783, Length=244, Percent_Identity=32.7868852459016, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=28.3636363636364, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789149, Length=231, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=25.7261410788382, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788549, Length=264, Percent_Identity=25.3787878787879, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=317, Percent_Identity=24.2902208201893, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=230, Percent_Identity=30.8695652173913, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081816, Length=287, Percent_Identity=26.4808362369338, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790551, Length=240, Percent_Identity=28.3333333333333, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789403, Length=341, Percent_Identity=28.1524926686217, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=27.6497695852535, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790861, Length=238, Percent_Identity=30.672268907563, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=22.9074889867841, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=244, Percent_Identity=25.8196721311475, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=295, Percent_Identity=25.0847457627119, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787374, Length=290, Percent_Identity=25.1724137931034, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48276; Mature: 48276

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRSTLKLSLMMLVAGAIAVQALSWSAGRLFHDTLTQGQREGRKGYVFLLREYLDRFPGE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
ARATAIQRLNNNATELFDMVEPDTVADLSTEQRRDLADGKLVVMTNRMDYYLPLRDGTVL
HHHHHHHHHCCCHHHHHHHCCCCHHHCCCHHHHHHCCCCEEEEEECCCEEEEECCCCEEE
HARFEDIGYYTAIKIIAFCLIAIATLLPILFWSWLLWRDLRALEEAARGFGGGMLSTRAH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LRRGSNVHALARQFNDMAERIQGSIQHQREMMNGISHELKTPIARLEFGIALLQSPVPED
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
QRKARFDALRSDVRELDELVTELLALSRLEQGATHLVLMRVTVGEWLDSVVANIADDVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
RQLTLAVHADFAPAHHVCDPRLVARALLNLIRNAMRYARQAITVRAEAGTYGSLCLTVED
CEEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEC
DGPGIPAAERARVFEPFYRLDASRDRHTGGFGLGLAIVRRIALVHGGEVRLDTGGSGGAR
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
FVLLLPAMPLPMQ
EEEEECCCCCCCC
>Mature Secondary Structure
MFRSTLKLSLMMLVAGAIAVQALSWSAGRLFHDTLTQGQREGRKGYVFLLREYLDRFPGE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
ARATAIQRLNNNATELFDMVEPDTVADLSTEQRRDLADGKLVVMTNRMDYYLPLRDGTVL
HHHHHHHHHCCCHHHHHHHCCCCHHHCCCHHHHHHCCCCEEEEEECCCEEEEECCCCEEE
HARFEDIGYYTAIKIIAFCLIAIATLLPILFWSWLLWRDLRALEEAARGFGGGMLSTRAH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LRRGSNVHALARQFNDMAERIQGSIQHQREMMNGISHELKTPIARLEFGIALLQSPVPED
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
QRKARFDALRSDVRELDELVTELLALSRLEQGATHLVLMRVTVGEWLDSVVANIADDVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
RQLTLAVHADFAPAHHVCDPRLVARALLNLIRNAMRYARQAITVRAEAGTYGSLCLTVED
CEEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEC
DGPGIPAAERARVFEPFYRLDASRDRHTGGFGLGLAIVRRIALVHGGEVRLDTGGSGGAR
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
FVLLLPAMPLPMQ
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]