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The map label for this gene is citA [H]

Identifier: 17547778

GI number: 17547778

Start: 3295987

End: 3297282

Strand: Direct

Name: citA [H]

Synonym: RSc3059

Alternate gene names: 17547778

Gene position: 3295987-3297282 (Clockwise)

Preceding gene: 17547777

Following gene: 17547779

Centisome position: 88.69

GC content: 67.28

Gene sequence:

>1296_bases
ATGCATACCCACAACCCCAACGAATCCAAGGCCCGTTCCATCTTCCGGGTGGTCAGCGGTAACTTCCTGGAGATGTACGA
CTTCATGGTCTACGGCTACTACGCCAAGGCCATCGCGGACACCTTCTTCCCGGCCGACAACGAGTTCCTCTCGCTGATGC
TGTCGCTGGTGACCTTCGGCGTCGGCTTCCTGATGCGGCCGCTGGGCGCGCTGATCCTGGGCGCCTACATCGACCGCCAC
GGCCGCCGCGCCGGCCTGATCCTGACGCTGGGGCTGATGGCCTGCGGCACGCTGCTGATCGCGCTGGTGCCGGGCTATGC
AACGATCGGCCTGGCGGCGCCGCTGCTGGTGCTGATCGGCCGCCTGCTGCAAGGCTTCTCGGCCGGCGTGGAGCTGGGCG
GGGTGTCGGTCTACCTGGCGGAAATCTCGACGCCGGGCCGCAAGGGCTTCTTCGTGAGCTGGCAATCGGCCAGCCAGCAG
GTGGCGGTGATGTTCGCCGCGCTGCTGGGCGTGCTGCTGTCGTTCCAGCTCTCGCCCAAGGAAATGGGTGAGTGGGGCTG
GCGCGTGCCGTTCCTGGTCGGCTGCCTGATCGTGCCGTTCCTGTTCCTCATCCGCCGCTCGCTGCAAGAGACGGAGGAGT
TCAAGCAGCGCAAGCACCGTCCGGCGCTGGGCGAGATCTTCCGCTCGATGGGCGAGAACTGGCGCCTGGTGGGCGCGGGC
ACGCTGCTGGTGGTGATGACCACGGTGTCGTTCTACCTGATCACGGCCTACACGCCGACCTTCGGCAAGAGCGTGCTGCA
CCTGTCGAACCTCGACAGCCTGATCGTCACGCTGTGCGTAGGCGCCTCCAACTTCTTCTGGCTGCCGGTGATGGGCTCGG
TGTCCGACCGCATCGGCCGCCGCCCGCTGCTGATCACCTTCACCGCGCTGACGCTGCTGACGGCCTATCCGGCGATGCAG
TGGCTGATCGCCGAGCCGTCGTTCGGCCGCCTGCTGGCCGTGGAGCTGTGGCTGTCGTTCCTGTACGGCAGCTATAACGG
CGCGATGGTGGTGACGCTCACCGAGATCATGCCGCCGGCGGTGCGCACCACCGGGTTCTCGCTGGCCTACAGCCTGGCGA
CCGCCATCTTCGGCGGCTTCACCCCGGCCATCGCCACCTGGCTGATCCACGCTACCGGCAACAAGGCCATGCCGGGCCTG
TGGCTGTCCTTCGCCGCCATCTGCGGCCTGGTCGCCACGCTGATCGTGGTCAAGCCGGCGGGCCGCCACGTGCACACCAC
CGTGACGACCGCCTGA

Upstream 100 bases:

>100_bases
TCCAGCACGCCATACCACATCACGACTTTCTACGAGGCGGTCCGCGCTGCACTGGCGGCCCGCCCGACTGTTGTACCCCA
AGCTGTTGAGTGAGACATCC

Downstream 100 bases:

>100_bases
CCTTCGCACGCAACGCCCGGGCAAGCCGGGCGGCATCGCTGCGCCGGAGCGCCGCGGCTTTCTTTGCCGCACGCGCACGG
CGCCGCGCGCAACGCTGATG

Product: sugar transporter transmembrane protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHTHNPNESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVGFLMRPLGALILGAYIDRH
GRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQ
VAVMFAALLGVLLSFQLSPKEMGEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAG
TLLVVMTTVSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGRRPLLITFTALTLLTAYPAMQ
WLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPAVRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGL
WLSFAAICGLVATLIVVKPAGRHVHTTVTTA

Sequences:

>Translated_431_residues
MHTHNPNESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVGFLMRPLGALILGAYIDRH
GRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQ
VAVMFAALLGVLLSFQLSPKEMGEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAG
TLLVVMTTVSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGRRPLLITFTALTLLTAYPAMQ
WLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPAVRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGL
WLSFAAICGLVATLIVVKPAGRHVHTTVTTA
>Mature_431_residues
MHTHNPNESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVGFLMRPLGALILGAYIDRH
GRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQ
VAVMFAALLGVLLSFQLSPKEMGEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAG
TLLVVMTTVSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGRRPLLITFTALTLLTAYPAMQ
WLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPAVRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGL
WLSFAAICGLVATLIVVKPAGRHVHTTVTTA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=31.5420560747664, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1788942, Length=393, Percent_Identity=32.5699745547074, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=30.3102625298329, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=30.3030303030303, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46929; Mature: 46929

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTHNPNESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGFLMRPLGALILGAYIDRHGRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQVAVMFAALLGVLLSFQLSPK
HHHHHHHCCCEECCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHH
EMGEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECC
TLLVVMTTVSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGR
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCC
RPLLITFTALTLLTAYPAMQWLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCH
VRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGLWLSFAAICGLVATLIVVKPA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRHVHTTVTTA
CCEEEEEEECC
>Mature Secondary Structure
MHTHNPNESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGFLMRPLGALILGAYIDRHGRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQVAVMFAALLGVLLSFQLSPK
HHHHHHHCCCEECCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHH
EMGEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECC
TLLVVMTTVSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGR
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCC
RPLLITFTALTLLTAYPAMQWLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCH
VRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGLWLSFAAICGLVATLIVVKPA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRHVHTTVTTA
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]