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The map label for this gene is citA [H]

Identifier: 17547779

GI number: 17547779

Start: 3297795

End: 3299096

Strand: Direct

Name: citA [H]

Synonym: RSc3060

Alternate gene names: 17547779

Gene position: 3297795-3299096 (Clockwise)

Preceding gene: 17547778

Following gene: 17547781

Centisome position: 88.74

GC content: 68.05

Gene sequence:

>1302_bases
ATGCATACCCACAACCCTAACGAATCCAAAGCCCGCGCCATCTTCCGGGTCGTCAGCGGCAACTTCCTCGAGATGTTCGA
CTTCATGGTCTACGGCTACTACGCCACGGCCATTGCAGCGACGTTCTTCCCCGCAGGCAACGCCTTCCTGTCGCTAATGC
TATCGCTGATGACCTTCGGCGCCGGCTTCCTGATGCGGCCGCTGGGCGCGATCGTCCTGGGCGCCTACATCGACCGCCAC
GGCCGCCGCGCCGGCCTGATCCTGACGCTGGTGCTGATGGCCTGCGGCACGATGCTGATCGCGCTGGTGCCGGGCTACGG
CACCATCGGCCTGGCCGCGCCGCTGCTGGTGCTGATCGGCCGCCTGCTGCAAGGCTTCTCGGCCGGCGGCGAGCCGGGCG
GGGTCTCGGTCTACCTGTCGGAAATCGCGACGCCGGGCCGCAAAGGCTTCTACGTGAGCTGGCAGTCCGGCAGCCAGCAG
GTGGCGGTGATGTTCGCCGCGCTGCTGGGCGTGCTGCTCTCGTTCAAGCTCTCGCCCAAGGAAATGGGCGAATGGGGCTG
GCGCGTGCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCTTCATCCGCCGCTCGCTGCGCGAGACGGAGGAGT
TCGAGCAGCGCAAGCACCGCCCGACCATGGGCGAGCTGCTCCGCTCGATGGGCGAGAACTGGCGCCTCGCGGGCGCAGGC
ACGCTGCTGGCCGTGATGACCACGGTGTCGTTCTACCTGATCACGGCCTACACGCCGACCTTCGGCCGGAACGTGCTGCA
CATGTCGGATTTCGACAGCCTGATCGTCACGCTGTGCGTGGGCGCGTCCAACCTCATCTGGCTGCCGGTGATGGGCTCGC
TGTCCGACCGCATCGGCCGCCGCACGCCGCTGATCGTCTTCAGCGCGCTGACGCTGCTGACGGCCTACCCGGCCATGACG
TGGCTGATCGCCGAGCCGTCGTTCGGCCGCCTGCTGGCGGTGGAGCTGTGGCTGTCGTTCCTGTACGGCGGCTACAACGG
CGCCATGATCGTCACGCTCACCGAGATCATGCCGCCGCAGCTGCGCACCACGGGCTTCTCGCTGGCCTACAGCCTGGCGA
CCGCCATCTTCGGCGGCTTCACCCCGGCCATCGCCACCGGGCTGATCCACGCCACCGGCAACAAGGCCATGCCGGGCCTG
TGGCTGTCCTTCGCCGCCATCTGCGGCCTGGTCGCCACGCTGACGGTGGTCAAGCCGCAGCCCCGTCCCGCCGCCATGCC
GGACCCCCTGCTCGAGCCGTAA

Upstream 100 bases:

>100_bases
CCCCCTTCGTGCCGCACCGCACCATCGCTTTCTGTGAGACGATCCCGGCCGGGCGGGCTGGCTCGCCTGACCGTTGTACC
AAGCTGTTCTGTCAAACGTC

Downstream 100 bases:

>100_bases
CGCGCCGAGAGACCCCAGCCACCCGGCGCACCGCCGCCGGGTGCCCGCCGGCAGACCGCTGCCGGCGGATCGCCTGACCT
CACGGGCGCCGCCTCCGGCG

Product: transporter transmembrane protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MHTHNPNESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAIVLGAYIDRH
GRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQ
VAVMFAALLGVLLSFKLSPKEMGEWGWRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWRLAGAG
TLLAVMTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLIVFSALTLLTAYPAMT
WLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGL
WLSFAAICGLVATLTVVKPQPRPAAMPDPLLEP

Sequences:

>Translated_433_residues
MHTHNPNESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAIVLGAYIDRH
GRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQ
VAVMFAALLGVLLSFKLSPKEMGEWGWRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWRLAGAG
TLLAVMTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLIVFSALTLLTAYPAMT
WLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGL
WLSFAAICGLVATLTVVKPQPRPAAMPDPLLEP
>Mature_433_residues
MHTHNPNESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAIVLGAYIDRH
GRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQ
VAVMFAALLGVLLSFKLSPKEMGEWGWRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWRLAGAG
TLLAVMTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLIVFSALTLLTAYPAMT
WLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGL
WLSFAAICGLVATLTVVKPQPRPAAMPDPLLEP

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=31.2354312354312, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788942, Length=394, Percent_Identity=35.5329949238579, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=30.2884615384615, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=31.5914489311164, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082231, Length=252, Percent_Identity=26.984126984127, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082404, Length=402, Percent_Identity=22.8855721393035, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46919; Mature: 46919

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTHNPNESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
AGFLMRPLGAIVLGAYIDRHGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLLQGFSAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPK
HHHHHHCCCCCCCCCEEEHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHH
EMGEWGWRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWRLAGAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEECHH
TLLAVMTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGR
HHHHHHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCC
RTPLIVFSALTLLTAYPAMTWLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQ
CCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCC
LRTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATLTVVKPQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRPAAMPDPLLEP
CCCCCCCCCCCCC
>Mature Secondary Structure
MHTHNPNESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
AGFLMRPLGAIVLGAYIDRHGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLLQGFSAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPK
HHHHHHCCCCCCCCCEEEHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHH
EMGEWGWRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWRLAGAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEECHH
TLLAVMTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGR
HHHHHHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCC
RTPLIVFSALTLLTAYPAMTWLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQ
CCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCC
LRTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATLTVVKPQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRPAAMPDPLLEP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]