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The map label for this gene is hisH

Identifier: 17547667

GI number: 17547667

Start: 3177409

End: 3178062

Strand: Reverse

Name: hisH

Synonym: RSc2948

Alternate gene names: 17547667

Gene position: 3178062-3177409 (Counterclockwise)

Preceding gene: 17547668

Following gene: 17547666

Centisome position: 85.51

GC content: 65.6

Gene sequence:

>654_bases
ATGACTACGATTGCAATTGTCGATTACGGCATGGGCAACCTGCGCTCGGTGGCGCAGGCCCTGAGTACCGTTGCCCCGGA
CGCCGATGTGCGCATCAGTGCGCAGGCCGACGAGATCCGGGCCGCCGACCGGGTGGTGCTGCCGGGGCAGGGCGCCATGC
CCGACTGCATGGCGGCCTTCGACCAGTCCGGACTGCGCGAAGCCGTGCTCGAGGCCGCGCGCACCAAGCCGATGCTCGGC
GTGTGCGTGGGCGAACAGATGCTGCTGGAGCGCAGCGCCGAGGCGCGCGCCGGCGAGGCGTTCACGGCGGGCCTGGGGCT
GATTTCCGGCGATGTGATCCGCTTCGACCTGGACGGCCGGTTGCAGCCGGACGGTTCGCGGTACAAAGTGCCTCAGATGG
GCTGGAACCGCGTGCACCAGAGCCGAACCCATGCGCTGTGGGCCGGCGTGCCGGACCAGAGCTACTTTTACTTCGTGCAC
AGTTACTATGCACGGCCGGCGCACCCCGACGAATCGGTCGGGGAGACCGAATACGGTGTGCGGTTTACCTGCGCCATTGC
CCGGGATAATATTTTCGCAACACAGTTTCACCCGGAAAAAAGTGCGCAGGCCGGCTTGCAGATCTACCGGAATTTCGTGC
ACTGGAAGCCCTGA

Upstream 100 bases:

>100_bases
GTATCAACGTCGGCACGCGCCTGATGGGGTTGATCCTCGCGGCGCTGGCCGTGGAATTCATCGTCGACGGGCTGAAAACC
CTGTTGCCTGTTCTCAAATC

Downstream 100 bases:

>100_bases
CGTCGTTCGTCACAGACGCACCCGTTTTTTCGAACCTGTTTTTTATTTTTCCCCCTCAACTTTGTCGTCACGCTTATGTT
GCTCATCCCGGCCATCGACC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTTIAIVDYGMGNLRSVAQALSTVAPDADVRISAQADEIRAADRVVLPGQGAMPDCMAAFDQSGLREAVLEAARTKPMLG
VCVGEQMLLERSAEARAGEAFTAGLGLISGDVIRFDLDGRLQPDGSRYKVPQMGWNRVHQSRTHALWAGVPDQSYFYFVH
SYYARPAHPDESVGETEYGVRFTCAIARDNIFATQFHPEKSAQAGLQIYRNFVHWKP

Sequences:

>Translated_217_residues
MTTIAIVDYGMGNLRSVAQALSTVAPDADVRISAQADEIRAADRVVLPGQGAMPDCMAAFDQSGLREAVLEAARTKPMLG
VCVGEQMLLERSAEARAGEAFTAGLGLISGDVIRFDLDGRLQPDGSRYKVPQMGWNRVHQSRTHALWAGVPDQSYFYFVH
SYYARPAHPDESVGETEYGVRFTCAIARDNIFATQFHPEKSAQAGLQIYRNFVHWKP
>Mature_216_residues
TTIAIVDYGMGNLRSVAQALSTVAPDADVRISAQADEIRAADRVVLPGQGAMPDCMAAFDQSGLREAVLEAARTKPMLGV
CVGEQMLLERSAEARAGEAFTAGLGLISGDVIRFDLDGRLQPDGSRYKVPQMGWNRVHQSRTHALWAGVPDQSYFYFVHS
YYARPAHPDESVGETEYGVRFTCAIARDNIFATQFHPEKSAQAGLQIYRNFVHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=37.0892018779343, Blast_Score=128, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=223, Percent_Identity=30.4932735426009, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_RALSO (Q8XV83)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_521069.1
- ProteinModelPortal:   Q8XV83
- SMR:   Q8XV83
- GeneID:   1221802
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2948
- NMPDR:   fig|267608.1.peg.2948
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13146
- BioCyc:   RSOL267608:RSC2948-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23850; Mature: 23719

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 197-197 ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAIVDYGMGNLRSVAQALSTVAPDADVRISAQADEIRAADRVVLPGQGAMPDCMAAF
CCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCEEEECCCCCCHHHHHHH
DQSGLREAVLEAARTKPMLGVCVGEQMLLERSAEARAGEAFTAGLGLISGDVIRFDLDGR
HHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCHHHHCCCEECCCEEEEECCCE
LQPDGSRYKVPQMGWNRVHQSRTHALWAGVPDQSYFYFVHSYYARPAHPDESVGETEYGV
ECCCCCEEECCCCCHHHHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
RFTCAIARDNIFATQFHPEKSAQAGLQIYRNFVHWKP
EEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIAIVDYGMGNLRSVAQALSTVAPDADVRISAQADEIRAADRVVLPGQGAMPDCMAAF
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCEEEECCCCCCHHHHHHH
DQSGLREAVLEAARTKPMLGVCVGEQMLLERSAEARAGEAFTAGLGLISGDVIRFDLDGR
HHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCHHHHCCCEECCCEEEEECCCE
LQPDGSRYKVPQMGWNRVHQSRTHALWAGVPDQSYFYFVHSYYARPAHPDESVGETEYGV
ECCCCCEEECCCCCHHHHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
RFTCAIARDNIFATQFHPEKSAQAGLQIYRNFVHWKP
EEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852