BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is paaF [H]

Identifier: 17547591

GI number: 17547591

Start: 3101636

End: 3102412

Strand: Reverse

Name: paaF [H]

Synonym: RSc2872

Alternate gene names: 17547591

Gene position: 3102412-3101636 (Counterclockwise)

Preceding gene: 17547595

Following gene: 17547589

Centisome position: 83.48

GC content: 64.74

Gene sequence:

>777_bases
ATGTCCTACCAACACATTCTGGTCGAAACGCGCGGTCGTGTCGGCCTTGTCACGCTCAACCGCCCCAAGGCGCTCAACGC
GCTCAACGATGCGCTGATGGACGAACTCGGCGCCGCGCTGCTCGCCTTCGATGCCGACGAGGGCATCGGCGCCATGATCA
TCACCGGTAGCGACAAGGCGTTTGCAGCTGGTGCGGACATCGGCGCCATGGCCGACTACGACTTCGCCACCGTCTATAAG
AATGAATACATCACGCGCAACTGGGAAACCATCCGGCGCATCCGCAAGCCGGTGATCGCCGCCGTGGCGGGCTACGCGCT
GGGCGGCGGCTGCGAGTTGGCGATGATGTGCGACATCATCCTGGCGGCGGACACCGCCAGGTTCGGCCAGCCGGAGATCA
AACTCGGCACCATGCCGGGCGCGGGCGGCACCCAGCGCCTGCCACGCGCCGTCTCCAAGGCCAAGGCCATGGACCTCTGC
CTGACCGCGCGCATGATGGGCGCCGATGAAGCTGAGCGCGCCGGCTTGGTTTCACGCGTTATCCCCGCCGACAAGCTCCT
GGATGAAGCCTTGGCGGCCGCCGAGACCATCGCCGGCTTCTCCCTGCCGACCGCCATGATGATCAAGGAGTCGATCAATG
CGGCATACGAAACCACGCTGAGCGAGGGCGTGCGCCTGGAGCGCCGCCTGTTCCACGCCACATTTGCCACGGAAGACCAG
AAAGAGGGCATGCACGCTTTCCTGGGGAAGCGGCAACCTGCTTTCAAACACCGCTAA

Upstream 100 bases:

>100_bases
CAGAATGGTCGGGCTGGTCATCTTGTCTCCTCCTCGGTCGCAGGTTGATTAACCGACCGGTTGGTCGGCTAATATTACAT
CGATTCCAACTGGAGTTCCC

Downstream 100 bases:

>100_bases
CAAGTGTCGGAGCGGACGCTTCCGAATGTCCGAGTCCCTGACTCAAGTATATCTGGGGTCACTTCACCCTGACTTTTGTC
CACAAACGTAGGTGGGCATT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYQHILVETRGRVGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSDKAFAAGADIGAMADYDFATVYK
NEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMGADEAERAGLVSRVIPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATEDQ
KEGMHAFLGKRQPAFKHR

Sequences:

>Translated_258_residues
MSYQHILVETRGRVGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSDKAFAAGADIGAMADYDFATVYK
NEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMGADEAERAGLVSRVIPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATEDQ
KEGMHAFLGKRQPAFKHR
>Mature_257_residues
SYQHILVETRGRVGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSDKAFAAGADIGAMADYDFATVYKN
EYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCL
TARMMGADEAERAGLVSRVIPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATEDQK
EGMHAFLGKRQPAFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=53.8461538461538, Blast_Score=291, Evalue=4e-79,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=35.9848484848485, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI68989263, Length=228, Percent_Identity=32.8947368421053, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=261, Percent_Identity=34.0996168582375, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=187, Percent_Identity=34.2245989304813, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI37594469, Length=202, Percent_Identity=30.6930693069307, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI37594471, Length=202, Percent_Identity=30.6930693069307, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=29.9180327868852, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI45643119, Length=245, Percent_Identity=27.7551020408163, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=245, Percent_Identity=27.7551020408163, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=245, Percent_Identity=27.7551020408163, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757968, Length=207, Percent_Identity=25.6038647342995, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=207, Percent_Identity=25.6038647342995, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=25.9574468085106, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=25.9574468085106, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4757966, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI51538581, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI25453481, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538573, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=34.0740740740741, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=27.2277227722772, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=46.4566929133858, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI221142681, Length=254, Percent_Identity=37.7952755905512, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=34.9397590361446, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=33.7164750957854, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=30.3846153846154, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=31.3432835820896, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=54.5816733067729, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.4979423868313, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=31.3492063492063, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=31.981981981982, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=35, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=37.9120879120879, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=37.9120879120879, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=37.9120879120879, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=29.5275590551181, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=28.3018867924528, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=34.5177664974619, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=34.5177664974619, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=31.8435754189944, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=24.7104247104247, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=27, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=55.5984555984556, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=55.5984555984556, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=34.6007604562738, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI21357171, Length=271, Percent_Identity=30.6273062730627, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=30.188679245283, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=26.0869565217391, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=31.7647058823529, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654903, Length=210, Percent_Identity=28.0952380952381, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583077, Length=189, Percent_Identity=31.2169312169312, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583079, Length=189, Percent_Identity=31.2169312169312, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921000, Length=189, Percent_Identity=31.2169312169312, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=193, Percent_Identity=31.0880829015544, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571729, Length=193, Percent_Identity=31.0880829015544, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27742; Mature: 27611

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQHILVETRGRVGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSDKA
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCC
FAAGADIGAMADYDFATVYKNEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDII
EECCCCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMGADEAERAGLVSRV
HHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATEDQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KEGMHAFLGKRQPAFKHR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYQHILVETRGRVGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSDKA
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCC
FAAGADIGAMADYDFATVYKNEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDII
EECCCCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMGADEAERAGLVSRV
HHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATEDQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KEGMHAFLGKRQPAFKHR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]