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The map label for this gene is yoaE [H]

Identifier: 17547578

GI number: 17547578

Start: 3078218

End: 3080314

Strand: Reverse

Name: yoaE [H]

Synonym: RSc2859

Alternate gene names: 17547578

Gene position: 3080314-3078218 (Counterclockwise)

Preceding gene: 17547579

Following gene: 17547577

Centisome position: 82.88

GC content: 71.15

Gene sequence:

>2097_bases
ATGTCCACCCAAGTCATCCGTGCCGCGTGTCCGCACGACTGCCCCGACACCTGCGCCTTGCTCGTCACCGTGGAGGGCGG
CCGCGCCACGCGCGTGCAGGGCGATCCGGAGCATCCGACCACGCAGGGCGTGCTCTGTACCAAGGTCAACCGCTACGCCG
AACGCACCTATCACCCCGACCGGCTGCTGACACCGATGAAGCGCATCGGCGCCAAGGGCGAGGGCAGGTTCGCGCCGATC
TCGTGGGACGAGGCGCTCGACACCATTGCCGCCCGCCTGGGCGGGATCGCGGCGCGCAGCCCGCAGGCCATCCTGCCGTA
CAGCTATGCCGGCACCATGGGCCTGGTGCAGGGTGAAAGCATGGCCGCGCGCTTCTTCCACAAGCTGGGTGCGTCGCTGC
TGGACCGCACCATCTGCGCCTCGGCCGGCGCGACGGCGCTGCGCTACACGTATGGCGCGAGCCTCGGCATGGACATCGAG
CACGTGCAGGACGCCCGGCTGATCCTGATCTGGGGCGGCAATCCGATCGCCTCCAACCTGCATTTCTGGACGCGCGCCCA
GGAGGCCAAGCGGCGCGGCGCCACGCTGGTGGCGATCGATCCGTATCGCTCGCTGACGGCCGAGAAGTGCCACCGGCATC
TGGCCGTGCGCCCCGGCACCGATGCGGCGCTGGCGCTGGCGATGATCCATGTGCTGATCCGTGACGACCTGCTCGACCAC
GGCTACATCGCCGATCACACGCTGGGCTTCGAGGCGCTGCGCGAGCGCGCGCGGCAGTACACGCCCGAGTACGCCGCCGC
GCTGTGCGGCATCGATGCCGCCGACATCGAATGGCTGGCCACGCTGTATGGCACCACCGCCGTGCGCGAGCGCCAGCCCG
TGGCCATCCGCCTGAACTACGGCATGCAGCGCGCCCACGGCGGCGGGCAGGGCGTGCGCGCGGTGGCGTGCCTGCCGTCG
CTGGTGGGCGCGTGGCGCGCGCCGGCCGGCGGGCTGCAGCTGTCGACCTCGGGCTTCTTCCCGATCGACACCGCGGCCTT
GCAGCGCCCTGACCTGCTGCCCGGCTGGCCCGCGCAGCCGCGCACCCTCAACATGAGCACCATCGGCGATGCGCTGCTGC
ACCCGGGCGATGCGTCGTTCGGCCCGAAGGTGGAGGCCGTGGTCGTCTACAACAGCAATCCGGTGGCGGTGGCGCCGGAA
TCGCGCAAGGTGGCCGCCGGCTTCGCGCGCGAAGATCTGTTCACGGTCGTGCTGGAGCACTTCCGGACCGACACCGCCGA
CTACGCCGACATCCTGCTGCCCGCCACCACGCAGCTCGAACACGTCGACGTGCACAAGGCCTACGGCCATACCTACATGC
TGGCCAACAACGCCGCCATCGCGCCGCTGGGCCAGGCGCTGCCGAACACGGAGATCTTCCGCCGCCTCGCGCAGCGCATG
GGGTTTGCCGATCCGTGCTTCGCCGATTCCGACGACGACATCGCCGCCCAGGCCCTGCGCCGCGGCGACCCGTCCGCCGC
CCACATCGACTGGGCCACGCTCAAGCGCGCGGGCTGGCAGAAGCTGGCGCACCCAGCCGCGCCGTTCGCGCGCGGCGGCT
TCCGCACGCCGTCCGGCCGCTGCGAGTTCTACTCCGAGCAGATGGCCCGCGACGGCCTCGACCCGCTGCCCGGCCATGTG
CCGCCGCACGAATCGCCCGTCAGCGCGCCGGAACTGGCCAGGCGCTATCCGCTGTCGATGATCTCGCCGCCGGCGCGCAA
CTTCCTCAACTCGACCTTCGTCAACGTGGACAGTCTGCGCAACACCGAGGGCGAACCGCATCTCGACATCCACCCGGCCG
ACGCGCACGCGCGCGGCGTGGCCGACGGCAGCATGGTTCGCGTCTTCAACGACCGCGGCACCATGCACGCCCGCGCCCGC
GTGACCGATCGCGCCCGGCCGGGCGTGGTGGTCGGCCTGTCGATCTGGTGGAAGAAGCTGGCGCCGGACGGTACCAACGC
CAACGAACTGACCAGCCAGCGCCTGACTGACATGGGCCGGGCCCCCACCTTCTACGACTGCCTGGTGGAGGTCGCACCGG
CGGCGCAGGCAGCCTGA

Upstream 100 bases:

>100_bases
CTGGTGGCGCGCGGCTCGACGCCGCAGCGACGCATGTAGCGGGCGATTACAATGCCCGGACGAAGACGGCACCGCCCGCC
GCCCCACTCCGGAGCTCCCG

Downstream 100 bases:

>100_bases
GGACCCGACCGGTATGCGCGGCTTTCGTGCGGGGGTGTTGGCGCTGTCCATGGCCGGCGCGGCGTTGCTGACCGGCTGCG
ATACGGTCGGCTATTACTAC

Product: oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MSTQVIRAACPHDCPDTCALLVTVEGGRATRVQGDPEHPTTQGVLCTKVNRYAERTYHPDRLLTPMKRIGAKGEGRFAPI
SWDEALDTIAARLGGIAARSPQAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIE
HVQDARLILIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
GYIADHTLGFEALRERARQYTPEYAAALCGIDAADIEWLATLYGTTAVRERQPVAIRLNYGMQRAHGGGQGVRAVACLPS
LVGAWRAPAGGLQLSTSGFFPIDTAALQRPDLLPGWPAQPRTLNMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPE
SRKVAAGFAREDLFTVVLEHFRTDTADYADILLPATTQLEHVDVHKAYGHTYMLANNAAIAPLGQALPNTEIFRRLAQRM
GFADPCFADSDDDIAAQALRRGDPSAAHIDWATLKRAGWQKLAHPAAPFARGGFRTPSGRCEFYSEQMARDGLDPLPGHV
PPHESPVSAPELARRYPLSMISPPARNFLNSTFVNVDSLRNTEGEPHLDIHPADAHARGVADGSMVRVFNDRGTMHARAR
VTDRARPGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPAAQAA

Sequences:

>Translated_698_residues
MSTQVIRAACPHDCPDTCALLVTVEGGRATRVQGDPEHPTTQGVLCTKVNRYAERTYHPDRLLTPMKRIGAKGEGRFAPI
SWDEALDTIAARLGGIAARSPQAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIE
HVQDARLILIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
GYIADHTLGFEALRERARQYTPEYAAALCGIDAADIEWLATLYGTTAVRERQPVAIRLNYGMQRAHGGGQGVRAVACLPS
LVGAWRAPAGGLQLSTSGFFPIDTAALQRPDLLPGWPAQPRTLNMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPE
SRKVAAGFAREDLFTVVLEHFRTDTADYADILLPATTQLEHVDVHKAYGHTYMLANNAAIAPLGQALPNTEIFRRLAQRM
GFADPCFADSDDDIAAQALRRGDPSAAHIDWATLKRAGWQKLAHPAAPFARGGFRTPSGRCEFYSEQMARDGLDPLPGHV
PPHESPVSAPELARRYPLSMISPPARNFLNSTFVNVDSLRNTEGEPHLDIHPADAHARGVADGSMVRVFNDRGTMHARAR
VTDRARPGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPAAQAA
>Mature_697_residues
STQVIRAACPHDCPDTCALLVTVEGGRATRVQGDPEHPTTQGVLCTKVNRYAERTYHPDRLLTPMKRIGAKGEGRFAPIS
WDEALDTIAARLGGIAARSPQAILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEH
VQDARLILIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDHG
YIADHTLGFEALRERARQYTPEYAAALCGIDAADIEWLATLYGTTAVRERQPVAIRLNYGMQRAHGGGQGVRAVACLPSL
VGAWRAPAGGLQLSTSGFFPIDTAALQRPDLLPGWPAQPRTLNMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPES
RKVAAGFAREDLFTVVLEHFRTDTADYADILLPATTQLEHVDVHKAYGHTYMLANNAAIAPLGQALPNTEIFRRLAQRMG
FADPCFADSDDDIAAQALRRGDPSAAHIDWATLKRAGWQKLAHPAAPFARGGFRTPSGRCEFYSEQMARDGLDPLPGHVP
PHESPVSAPELARRYPLSMISPPARNFLNSTFVNVDSLRNTEGEPHLDIHPADAHARGVADGSMVRVFNDRGTMHARARV
TDRARPGVVVGLSIWWKKLAPDGTNANELTSQRLTDMGRAPTFYDCLVEVAPAAQAA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=776, Percent_Identity=27.0618556701031, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1787870, Length=784, Percent_Identity=27.2959183673469, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI171474008, Length=765, Percent_Identity=26.0130718954248, Blast_Score=192, Evalue=9e-50,
Organism=Escherichia coli, GI3868721, Length=730, Percent_Identity=25.7534246575342, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1787231, Length=689, Percent_Identity=25.689404934688, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI145693196, Length=658, Percent_Identity=25.8358662613982, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI87081994, Length=684, Percent_Identity=24.4152046783626, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788534, Length=312, Percent_Identity=20.5128205128205, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI3868719, Length=570, Percent_Identity=21.2280701754386, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI3868720, Length=277, Percent_Identity=26.3537906137184, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75565; Mature: 75433

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQVIRAACPHDCPDTCALLVTVEGGRATRVQGDPEHPTTQGVLCTKVNRYAERTYHPD
CCCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPMKRIGAKGEGRFAPISWDEALDTIAARLGGIAARSPQAILPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEHVQDARLILIWGGNPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
GYIADHTLGFEALRERARQYTPEYAAALCGIDAADIEWLATLYGTTAVRERQPVAIRLNY
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
GMQRAHGGGQGVRAVACLPSLVGAWRAPAGGLQLSTSGFFPIDTAALQRPDLLPGWPAQP
CCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCC
RTLNMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPESRKVAAGFAREDLFTVVLEH
CEEEHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHH
FRTDTADYADILLPATTQLEHVDVHKAYGHTYMLANNAAIAPLGQALPNTEIFRRLAQRM
HCCCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHC
GFADPCFADSDDDIAAQALRRGDPSAAHIDWATLKRAGWQKLAHPAAPFARGGFRTPSGR
CCCCCCCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCHHCCCCCCCCCC
CEFYSEQMARDGLDPLPGHVPPHESPVSAPELARRYPLSMISPPARNFLNSTFVNVDSLR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHEEHHHHC
NTEGEPHLDIHPADAHARGVADGSMVRVFNDRGTMHARARVTDRARPGVVVGLSIWWKKL
CCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHH
APDGTNANELTSQRLTDMGRAPTFYDCLVEVAPAAQAA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STQVIRAACPHDCPDTCALLVTVEGGRATRVQGDPEHPTTQGVLCTKVNRYAERTYHPD
CCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPMKRIGAKGEGRFAPISWDEALDTIAARLGGIAARSPQAILPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASLGMDIEHVQDARLILIWGGNPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHLAVRPGTDAALALAMIHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
GYIADHTLGFEALRERARQYTPEYAAALCGIDAADIEWLATLYGTTAVRERQPVAIRLNY
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
GMQRAHGGGQGVRAVACLPSLVGAWRAPAGGLQLSTSGFFPIDTAALQRPDLLPGWPAQP
CCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCC
RTLNMSTIGDALLHPGDASFGPKVEAVVVYNSNPVAVAPESRKVAAGFAREDLFTVVLEH
CEEEHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHH
FRTDTADYADILLPATTQLEHVDVHKAYGHTYMLANNAAIAPLGQALPNTEIFRRLAQRM
HCCCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHC
GFADPCFADSDDDIAAQALRRGDPSAAHIDWATLKRAGWQKLAHPAAPFARGGFRTPSGR
CCCCCCCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCHHCCCCCCCCCC
CEFYSEQMARDGLDPLPGHVPPHESPVSAPELARRYPLSMISPPARNFLNSTFVNVDSLR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHEEHHHHC
NTEGEPHLDIHPADAHARGVADGSMVRVFNDRGTMHARARVTDRARPGVVVGLSIWWKKL
CCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHH
APDGTNANELTSQRLTDMGRAPTFYDCLVEVAPAAQAA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]