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The map label for this gene is murF [H]

Identifier: 17547567

GI number: 17547567

Start: 3064565

End: 3066013

Strand: Reverse

Name: murF [H]

Synonym: RSc2848

Alternate gene names: 17547567

Gene position: 3066013-3064565 (Counterclockwise)

Preceding gene: 17547568

Following gene: 17547566

Centisome position: 82.5

GC content: 71.64

Gene sequence:

>1449_bases
ATGAGCGATGCACTGCCCGTGATGATGCACGCCACCGATGCCGCCGCCTGGATGGCCGGCGCGCACCTGGTCGGCCCGGA
CGCCGCCATCCGCCGCGTCACCACCGACAGCCGCGCCGTGCTGCCCGGCGACCTGTTCGTCGCGCTGATCGGCGAGCGCT
TCGACGCGCACGATTTTCTGCCGGATGTGGTGGCGCGCGGGGCCGCGGCAGTGCTGGTCTCGCGCCCGCCGGTCGGCATC
CCCGACCTGTCCGGCGTGGCGGTGCTGACCGTTGCCGATACGCGCATCGCGCTGGGCCAGCTGGCCGCCGGCTGGCGCCG
CCGGTTCCCGATCCCGGTGGTGGCGGTGACGGGCAGCAACGGCAAGACCACGGTCAAGGAAATGATCTCGGCCGTCTTCG
CGTGTGCCGTCGGTGAAGCGGCCCGCCTGGCGACGGCCGGCAACCTCAACAACGACATCGGCCTGCCGCTGACGCTGTTC
CGCCTGACCGCGCAGCACCGGCTGGCCGTGCTCGAGCTGGGCATGAACCATCCCGGCGAAACCGAGGTGCTGGCCGCCAT
CGCCCAGCCCACCGTGGCGCTGATCAACAACGCACAGCGCGAGCACCAGGAGTTCATGGTCAGCGTGGAAGCCGTGGCGG
CCGAGCACGCCGCGGTGCTGGCCGCGCTGCCGGCCGACGGCGTGGCGGTTTTTCCGCGCGATGCGGCCAATGGCGGCGAA
TACGCGCCGCTGTGGCGCGCAGCGGCGGGTACGCGGCGTGTGCTGGATTTCGGCATCGAAGGCGGTGCGGTGTCGGCTGC
CGTGACGGACACGGCCGACGGCCAGCGGATCGACGTGCACGCGCCAGGCCTGCGCTTTTCCTTCACGCTGCCGTTACTTG
GTGTGCACAACGCGCGCAACGCGCTGGCGGCGACGGCCTGCGCGCTGGCCGCCGGCATCGCGCCCGAGGTGATCGCACAG
GCGCTGTCGGCGTTCGCGCCGGTCAAGGGGCGCCTGCAGCGCAAGCCGGGCCATCATGGCGGGCTGGTGATCGACGACAC
CTACAACGCGAACCCCGACTCCGTGCGCGCCGCCATCGATGCGTTGGCGACGCTGCCGGCGCCGCGCTGGCTGGTGCTGG
GCGACATGGGCGAAGTGGGCACGCAGGGGCCGGCCTTCCATCGGGAGATCGGCGCGTACGCCCGCGAGCGCGGCATCGAC
GCGGTCCTGGCCACCGGTGAGCTGGCGCGCCATATGGTGGAGGCTTTCGGTGGCGGCGCGCGGCATTTCGCGTCGGCCGA
GGCCCTGATCGAGCAGGGCGTGCCGGCCATTGCGCCGGGCGCGACGGTTCTGGTGAAGGGCTCGCGCTTCATGCGGATGG
AACGCATTGTCGATGCGCTGGTCTTGAAAGACCCGGCAGCCGACTCGCAAGGGGCGCCGGGGACGACGCACACACAACAG
ACGCACTAA

Upstream 100 bases:

>100_bases
GCAAGGGGCACGAATCGACGCAGGAAATTGCCGGGCGCAAGCGGCCGTTCTCCGATCAGGAGCACGTTCGCCTGGCGCTG
GCGGCACGGGGGGTGAACGC

Downstream 100 bases:

>100_bases
GGGATGTAGCACCCATGTTATTGGCATTGGCGCAGTGGCTGCAGAACGACTTCGGCTTCCTGCGCGTCTTCAACTATCTG
ACGTTCCGGGCCGTGATGGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFLPDVVARGAAAVLVSRPPVGI
PDLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSNGKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLF
RLTAQHRLAVLELGMNHPGETEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGE
YAPLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARNALAATACALAAGIAPEVIAQ
ALSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAIDALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGID
AVLATGELARHMVEAFGGGARHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQ
TH

Sequences:

>Translated_482_residues
MSDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFLPDVVARGAAAVLVSRPPVGI
PDLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSNGKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLF
RLTAQHRLAVLELGMNHPGETEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGE
YAPLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARNALAATACALAAGIAPEVIAQ
ALSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAIDALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGID
AVLATGELARHMVEAFGGGARHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQ
TH
>Mature_481_residues
SDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFLPDVVARGAAAVLVSRPPVGIP
DLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSNGKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLFR
LTAQHRLAVLELGMNHPGETEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGEY
APLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARNALAATACALAAGIAPEVIAQA
LSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAIDALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGIDA
VLATGELARHMVEAFGGGARHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQT
H

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=446, Percent_Identity=41.0313901345291, Blast_Score=264, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49932; Mature: 49800

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFL
CCCCCEEEEECCHHHHHHHCCEEECCHHHHHEECCCCCEECCHHHHHHHHHCCCCCHHHH
PDVVARGAAAVLVSRPPVGIPDLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSN
HHHHHCCCEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCC
GKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLFRLTAQHRLAVLELGMNHPGE
CCHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCH
TEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCC
YAPLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARN
CCHHHHHHCCCHHHEEECCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCEEECCCHHH
ALAATACALAAGIAPEVIAQALSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAID
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCCCCCHHHHHHHHH
ALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGIDAVLATGELARHMVEAFGGGA
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCH
RHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQ
HHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TH
CC
>Mature Secondary Structure 
SDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFL
CCCCEEEEECCHHHHHHHCCEEECCHHHHHEECCCCCEECCHHHHHHHHHCCCCCHHHH
PDVVARGAAAVLVSRPPVGIPDLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSN
HHHHHCCCEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCC
GKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLFRLTAQHRLAVLELGMNHPGE
CCHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCH
TEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCC
YAPLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARN
CCHHHHHHCCCHHHEEECCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCEEECCCHHH
ALAATACALAAGIAPEVIAQALSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAID
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCCCCCHHHHHHHHH
ALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGIDAVLATGELARHMVEAFGGGA
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCH
RHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQ
HHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]