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The map label for this gene is murE

Identifier: 17547568

GI number: 17547568

Start: 3066010

End: 3067554

Strand: Reverse

Name: murE

Synonym: RSc2849

Alternate gene names: 17547568

Gene position: 3067554-3066010 (Counterclockwise)

Preceding gene: 17547569

Following gene: 17547567

Centisome position: 82.54

GC content: 71.78

Gene sequence:

>1545_bases
ATGACGGCCGTGCCGACCTCCGAACGAGCGCCGATCGCTGCGCGCCTGGCACCGGTGCTGGACTGGCTGCGCGCGCACGT
GCCGGCCGGCGCCGATCTGACCAGCGATACGCGCAAGCTCAAGACCGGCGATGTGTTCGTCGCCTACGTGCTCGGCAACG
TGCGCCAGCGCGGCGACGGCCGGCCGCACATTCCGCAGGCCATCGAAGCCGGCGTCAGCGCCGTGCTGGCCGAGGCGCAC
GGTTACGTGGTGCCGGCCGATGCGCCGGCGCGCATCCTGCCGGTGGACGGCCTGTCCGAACTGGCCGGGCCGCTGGCCGC
GCAGTGGTATGCCGTGCCGGGGCCGGATGCCCTGCGCGTGATCGGCGTGACGGGCACCAACGGCAAGACGTCGTGCTCGC
AATGGATCGCCCAGGCGCTGAGCCGGCAGGGCGAGCGCTGCGCAGTGGTGGGTACGCTCGGCACCGGGTTTGTCGATGCG
CTGGTCGCCACGGGCTTCACCACGCCGGATGCCATCCAGCTCCAGCACAGCCTGGCCGACCTGCATCGCGCCGGTGCCCG
CGCCGTCGCCATGGAGGTGTCGTCGCACGGCCTGGAGCAGGGCCGCGCGGACGGCACCCGCTTCGACATCGCGCTGTTCA
CCAACCTGACGCAGGATCACCTCGACTACCACGGCACCATGGCCGAGTACGAGCTCGCCAAGGCGCGCCTGTTCGGCTGG
CCGGGCCTGCGCGCCGCCGTGATCAATCGCGACGACGCGGCGGGCGGGCGCCTGCTGGCCGGCCTGCGCGCCGGCATCGA
GGCGGTCGAATACGGCATCGACGGCGAAGCGGCCGCGCAGCGTGGCGCCGGGCACTGGCTGCGCGCCACCAACGTGCGCG
CGCACCGCACCGGTACGACCTTCGACGTCGACGGCAGCTTCGGCCGGGCGACCGTGCATTCGCCGATGGTCGGCCTGTTC
AACGTTTCGAACCAACTGGGCGTGCTGGGCGTGCTGCTGATGGCCGGCGTGCCATGGCAGGACGCGCTTGCCCGGCTGGA
AAAGCTGCAGCCGGTGAGCGGCCGCATGGAGCGCTTCGGCGGCGAGGACGGCCCGCTGGTGGTGGTCGACTACGCCCACA
CGCCCGATGCCCTGGAGCAGACGCTGCGTGCACTCGCGCCGATCACCGGGGCGCGGGGCGGCAAGCTGTGGGCGGTGTTC
GGCTGCGGCGGCGACCGCGATCCGGGCAAGCGCCCGCAGATGGGCGCCATCGCCGAGCGGCTGGCGCAGCACGTGGTGCT
GACCTCCGACAACCCGCGCAGCGAAGACCCGCAACTGATTCTCGACATGATCGCCGACGGCATGGAAGACCCGCGTCTCG
CCATACAGATCGAAGACCGCGCTGCCGCCATCCTGCACGCGGTGCGGCACGCCGATGTCCATGACGTCATCGTCGTGGCC
GGCAAGGGGCACGAATCGACGCAGGAAATTGCCGGGCGCAAGCGGCCGTTCTCCGATCAGGAGCACGTTCGCCTGGCGCT
GGCGGCACGGGGGGTGAACGCATGA

Upstream 100 bases:

>100_bases
CCGGCGGCAGCCTGCGCGCGCTGAACGTATCGCCGGATTCGCCGGTGCGCCAGCTCGTGATGACCGAAGGCGTGGCCGAA
GAACCCGTGGGGGCGCTGCA

Downstream 100 bases:

>100_bases
GCGATGCACTGCCCGTGATGATGCACGCCACCGATGCCGCCGCCTGGATGGCCGGCGCGCACCTGGTCGGCCCGGACGCC
GCCATCCGCCGCGTCACCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTAVPTSERAPIAARLAPVLDWLRAHVPAGADLTSDTRKLKTGDVFVAYVLGNVRQRGDGRPHIPQAIEAGVSAVLAEAH
GYVVPADAPARILPVDGLSELAGPLAAQWYAVPGPDALRVIGVTGTNGKTSCSQWIAQALSRQGERCAVVGTLGTGFVDA
LVATGFTTPDAIQLQHSLADLHRAGARAVAMEVSSHGLEQGRADGTRFDIALFTNLTQDHLDYHGTMAEYELAKARLFGW
PGLRAAVINRDDAAGGRLLAGLRAGIEAVEYGIDGEAAAQRGAGHWLRATNVRAHRTGTTFDVDGSFGRATVHSPMVGLF
NVSNQLGVLGVLLMAGVPWQDALARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITGARGGKLWAVF
GCGGDRDPGKRPQMGAIAERLAQHVVLTSDNPRSEDPQLILDMIADGMEDPRLAIQIEDRAAAILHAVRHADVHDVIVVA
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA

Sequences:

>Translated_514_residues
MTAVPTSERAPIAARLAPVLDWLRAHVPAGADLTSDTRKLKTGDVFVAYVLGNVRQRGDGRPHIPQAIEAGVSAVLAEAH
GYVVPADAPARILPVDGLSELAGPLAAQWYAVPGPDALRVIGVTGTNGKTSCSQWIAQALSRQGERCAVVGTLGTGFVDA
LVATGFTTPDAIQLQHSLADLHRAGARAVAMEVSSHGLEQGRADGTRFDIALFTNLTQDHLDYHGTMAEYELAKARLFGW
PGLRAAVINRDDAAGGRLLAGLRAGIEAVEYGIDGEAAAQRGAGHWLRATNVRAHRTGTTFDVDGSFGRATVHSPMVGLF
NVSNQLGVLGVLLMAGVPWQDALARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITGARGGKLWAVF
GCGGDRDPGKRPQMGAIAERLAQHVVLTSDNPRSEDPQLILDMIADGMEDPRLAIQIEDRAAAILHAVRHADVHDVIVVA
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
>Mature_513_residues
TAVPTSERAPIAARLAPVLDWLRAHVPAGADLTSDTRKLKTGDVFVAYVLGNVRQRGDGRPHIPQAIEAGVSAVLAEAHG
YVVPADAPARILPVDGLSELAGPLAAQWYAVPGPDALRVIGVTGTNGKTSCSQWIAQALSRQGERCAVVGTLGTGFVDAL
VATGFTTPDAIQLQHSLADLHRAGARAVAMEVSSHGLEQGRADGTRFDIALFTNLTQDHLDYHGTMAEYELAKARLFGWP
GLRAAVINRDDAAGGRLLAGLRAGIEAVEYGIDGEAAAQRGAGHWLRATNVRAHRTGTTFDVDGSFGRATVHSPMVGLFN
VSNQLGVLGVLLMAGVPWQDALARLEKLQPVSGRMERFGGEDGPLVVVDYAHTPDALEQTLRALAPITGARGGKLWAVFG
CGGDRDPGKRPQMGAIAERLAQHVVLTSDNPRSEDPQLILDMIADGMEDPRLAIQIEDRAAAILHAVRHADVHDVIVVAG
KGHESTQEIAGRKRPFSDQEHVRLALAARGVNA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=474, Percent_Identity=45.1476793248945, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_RALSO (Q8XVI2)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520970.1
- ProteinModelPortal:   Q8XVI2
- SMR:   Q8XVI2
- GeneID:   1221697
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2849
- NMPDR:   fig|267608.1.peg.2849
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   RSOL267608:RSC2849-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54391; Mature: 54260

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: BINDING 37-37 BINDING 194-194 BINDING 200-200 BINDING 202-202 BINDING 406-406 BINDING 481-481 BINDING 485-485

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVPTSERAPIAARLAPVLDWLRAHVPAGADLTSDTRKLKTGDVFVAYVLGNVRQRGDG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEHHHHHHCCCC
RPHIPQAIEAGVSAVLAEAHGYVVPADAPARILPVDGLSELAGPLAAQWYAVPGPDALRV
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHHHCEEECCCCCCEEE
IGVTGTNGKTSCSQWIAQALSRQGERCAVVGTLGTGFVDALVATGFTTPDAIQLQHSLAD
EEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LHRAGARAVAMEVSSHGLEQGRADGTRFDIALFTNLTQDHLDYHGTMAEYELAKARLFGW
HHHCCCCEEEEEHHHCCHHCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCC
PGLRAAVINRDDAAGGRLLAGLRAGIEAVEYGIDGEAAAQRGAGHWLRATNVRAHRTGTT
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEEECCCCE
FDVDGSFGRATVHSPMVGLFNVSNQLGVLGVLLMAGVPWQDALARLEKLQPVSGRMERFG
EECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCC
GEDGPLVVVDYAHTPDALEQTLRALAPITGARGGKLWAVFGCGGDRDPGKRPQMGAIAER
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LAQHVVLTSDNPRSEDPQLILDMIADGMEDPRLAIQIEDRAAAILHAVRHADVHDVIVVA
HHHEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEE
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
CCCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCC
>Mature Secondary Structure 
TAVPTSERAPIAARLAPVLDWLRAHVPAGADLTSDTRKLKTGDVFVAYVLGNVRQRGDG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEHHHHHHCCCC
RPHIPQAIEAGVSAVLAEAHGYVVPADAPARILPVDGLSELAGPLAAQWYAVPGPDALRV
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHHHCEEECCCCCCEEE
IGVTGTNGKTSCSQWIAQALSRQGERCAVVGTLGTGFVDALVATGFTTPDAIQLQHSLAD
EEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LHRAGARAVAMEVSSHGLEQGRADGTRFDIALFTNLTQDHLDYHGTMAEYELAKARLFGW
HHHCCCCEEEEEHHHCCHHCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCC
PGLRAAVINRDDAAGGRLLAGLRAGIEAVEYGIDGEAAAQRGAGHWLRATNVRAHRTGTT
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEEECCCCE
FDVDGSFGRATVHSPMVGLFNVSNQLGVLGVLLMAGVPWQDALARLEKLQPVSGRMERFG
EECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCC
GEDGPLVVVDYAHTPDALEQTLRALAPITGARGGKLWAVFGCGGDRDPGKRPQMGAIAER
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LAQHVVLTSDNPRSEDPQLILDMIADGMEDPRLAIQIEDRAAAILHAVRHADVHDVIVVA
HHHEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEE
GKGHESTQEIAGRKRPFSDQEHVRLALAARGVNA
CCCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852