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The map label for this gene is pilD [H]

Identifier: 17547546

GI number: 17547546

Start: 3041557

End: 3042441

Strand: Direct

Name: pilD [H]

Synonym: RSc2827

Alternate gene names: 17547546

Gene position: 3041557-3042441 (Clockwise)

Preceding gene: 17547545

Following gene: 17547547

Centisome position: 81.84

GC content: 69.04

Gene sequence:

>885_bases
ATGATGCCCGGAATGTTCGTCATTCCCACGCTGGCGCAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGG
CTTGCTGGTCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCG
CCGAGCTGCGCAACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGC
CACCAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTACGCGGCCGCTGCAGCGCCTGCAAGACCCC
CATCTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGCGCCTGCCTGTGGCACTTCGGACCGACCT
GGGTAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTCCTGCCC
GATGCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCAGCATGTTCGCGCGGCTGCCGGACGCGGT
AATCGGCGCGACGGCGGGGTATCTGTTCCTGTGGGCCATCTACTGGGCCTATAAGCTCCTGCGCGGCCGGGAAGGCATGG
GCTACGGCGACTTCAAGCTGATGGGCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCG
TCGGTGGTCGGCCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTCTCGTTCGGCCCGTT
CATCGCGGGTGCCGGCGTGATCGCGCTGCTGGCCGGCCCGCAACTGGTGGCGCTGACGGGGCTCGCGCCACTGCTGGCGC
GGTAA

Upstream 100 bases:

>100_bases
CCCTGATCGAGCCGCTCATCATCGTGATCCTGGGCGTGCTGATCGGGGGCATGGTGGTTGCCATGTACCTGCCGATCTTC
AAGCTGGGCCAAGTGGTCTG

Downstream 100 bases:

>100_bases
GAGGCTGCCTGCATGCGCGTGATCGGCCTGACCGGCGGCATCGGCAGCGGCAAGAGCTACGTTGCGGAGCGCCTTGCCGA
ACGGGGTGCCGCGGTCGTCG

Product: type 4 prepilin peptidase bifunctionnal: leader peptidase and N-methyltransferase transmembrane protein

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MMPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCG
HQIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLP
DAITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLS
SVVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Sequences:

>Translated_294_residues
MMPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCG
HQIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLP
DAITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLS
SVVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR
>Mature_294_residues
MMPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCG
HQIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLP
DAITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLS
SVVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=254, Percent_Identity=37.4015748031496, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=46.2121212121212, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31705; Mature: 31705

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEP
CCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPHPEPYNLVVPRSACPSCGHQIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTG
CCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHH
VLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPDAITQPLLWLGLLVNLFSMF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEP
CCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPHPEPYNLVVPRSACPSCGHQIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTG
CCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHH
VLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPDAITQPLLWLGLLVNLFSMF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA