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The map label for this gene is pilB [H]

Identifier: 17547544

GI number: 17547544

Start: 3038520

End: 3040247

Strand: Direct

Name: pilB [H]

Synonym: RSc2825

Alternate gene names: 17547544

Gene position: 3038520-3040247 (Clockwise)

Preceding gene: 17547543

Following gene: 17547545

Centisome position: 81.76

GC content: 63.31

Gene sequence:

>1728_bases
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCTATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Upstream 100 bases:

>100_bases
GAGCGCCGACAGCCAAAAACACAACAACTCGCCTCGGGGGGCGGGCGGGCGATCCGGTAGTGGGACCGGGCGCGCGCTGG
ATGGTCAGCCAGACGGACTC

Downstream 100 bases:

>100_bases
CCTTCTCGATCAATCGTTCCCATACCGGGGGGTAGGAACCAAACATGGCCACACGAGCACCGACCGCGAACCGGTCCGCC
GCCGCATCGGCACGCAAGAC

Product: type IV pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Sequences:

>Translated_575_residues
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ
>Mature_574_residues
TLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHFQ
QLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPGA
AAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRLD
ISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLDV
IKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMV
GEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETLL
DAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVKQ
GLTSLEEVLATTNQ

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=407, Percent_Identity=41.7690417690418, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1789723, Length=405, Percent_Identity=42.7160493827161, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI87082188, Length=254, Percent_Identity=32.2834645669291, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 63772; Mature: 63640

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLL
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
DLAQYKLDTVPPVLSANRHFQQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIE
HHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
TVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPGAAAAVRRTPTDGIDDAPVVR
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEECCCCCHHEECCCCCCCCCCHHHH
FLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESST
CCHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERLDIDQLGYEPEQKQLLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTA
CCCCHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCEEEECC
EDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADISIKAAQTG
CCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCC
HLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
CEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHH
EQARKEGVLSLRDSGLRKVKQGLTSLEEVLATTNQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLL
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
DLAQYKLDTVPPVLSANRHFQQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIE
HHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
TVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPGAAAAVRRTPTDGIDDAPVVR
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEECCCCCHHEECCCCCCCCCCHHHH
FLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESST
CCHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERLDIDQLGYEPEQKQLLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTA
CCCCHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCEEEECC
EDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADISIKAAQTG
CCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCC
HLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
CEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHH
EQARKEGVLSLRDSGLRKVKQGLTSLEEVLATTNQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]