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The map label for this gene is phcA

Identifier: 17547467

GI number: 17547467

Start: 2958349

End: 2959392

Strand: Reverse

Name: phcA

Synonym: RSc2748

Alternate gene names: 17547467

Gene position: 2959392-2958349 (Counterclockwise)

Preceding gene: 17547468

Following gene: 17547465

Centisome position: 79.63

GC content: 63.7

Gene sequence:

>1044_bases
ATGGTCAACGTCGATACCAAGCTGTTGGTGATTTTTGTAGAGCTGCTCAGCAAGCGGAACGCCACCTACGTCGCGGAAAA
GATGCACATGACGGCGCCCGCGGTATCGCACTCGCTGGGCCGCCTGCGGGAAATCTTCGACGATCCCCTCTTCATCCGCG
TGCCGCACGGCCTGACGCCGACGCCTAAAGCGCTTGAGCTCGGTCCCAAGGTGCGCGAGATGCTCGACCTGTGGGCGGCG
ATCAACGAAGGCGATATCGCCACGTTCGATCCCCTGGAAGCCGCCGGCACCTTCAACATCAGCTTCGCCGGCACGCTGGG
CGATGCCCTGTTCGACCGCTTCCTGCTGCGCGTCAAGCGCCTGGCGCCGGGTCTGCAGGTGCGCCTGACCGAGTCCTCCT
CGTGGGAAGCCGACGTGGCGGCGATGCGTTCCAATGAGCTGGACCTGGCGTTCTCCCCGTTCCCGACGCGGCATCCGGAA
ATCGTGGAAGAGGTCGTGACCTCCTTCAACATGTGGGTCTGCGCGCGTAAGGACCATCCGGTCCTGAAGGATCACTGCTC
GCTCGATCAGTACCTCGAATGCGAGCACATCTTCATCGCGCAGGGCAACCCCGGCACCCGCGCGGCGCCGTCGCTGATTC
CGCTCGATTACGCGCTGCAGCAGCGCGGCCTGAAGCGCCACTCCACCATGACTGTGCATGCATGGCGGACCCAGGCCGAG
GTGGCGGCGCAGACGGACATGATCTTCACGGTCAACTCGCTGATGAAGGATCTGGTGTGCGAGACCTACAACCTCAACGC
CTTCCCGCTGCCGTCCGAACTGGAAACCGTGCTGGGCCTGAACATGCTGTGGCACCGCAGCCGCAACACGCACCCGATGC
TGGTGTGGGCACGCAACCTGTTCAAGCAGGTGGTGGCCGAATACACCGGCAAGGCCTCCATCGCGCCGATGCACCCGCCC
ATGCTGACCGACGATTCCGGCAAGAGCGGCAAGACCGGTAAGGGCGATGCAGAAAAGGAGGATGAGTCGCGGCTGTCCGT
CTGA

Upstream 100 bases:

>100_bases
AATTACTACATTTGTGACGCAGTCGCCGTATCCTGCTGCGTCTGGGCCGTTGCCTCGGCAACGTCTGCCTTTCCACCTTC
TGTCTGAGCCAAAGCGTCCC

Downstream 100 bases:

>100_bases
TGCCAGCACCGCGCTCCTCCAACGAAAACGGCCGCAGTGGATGCGGCCGTTTTTTTTGATGAGGCGGCTGTTGCGTCAGC
GCTTGGGCCGGTGCGGGCAG

Product: transcription regulator protein

Products: NA

Alternate protein names: Virulence genes transcriptional regulator phcA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MVNVDTKLLVIFVELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPKALELGPKVREMLDLWAA
INEGDIATFDPLEAAGTFNISFAGTLGDALFDRFLLRVKRLAPGLQVRLTESSSWEADVAAMRSNELDLAFSPFPTRHPE
IVEEVVTSFNMWVCARKDHPVLKDHCSLDQYLECEHIFIAQGNPGTRAAPSLIPLDYALQQRGLKRHSTMTVHAWRTQAE
VAAQTDMIFTVNSLMKDLVCETYNLNAFPLPSELETVLGLNMLWHRSRNTHPMLVWARNLFKQVVAEYTGKASIAPMHPP
MLTDDSGKSGKTGKGDAEKEDESRLSV

Sequences:

>Translated_347_residues
MVNVDTKLLVIFVELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPKALELGPKVREMLDLWAA
INEGDIATFDPLEAAGTFNISFAGTLGDALFDRFLLRVKRLAPGLQVRLTESSSWEADVAAMRSNELDLAFSPFPTRHPE
IVEEVVTSFNMWVCARKDHPVLKDHCSLDQYLECEHIFIAQGNPGTRAAPSLIPLDYALQQRGLKRHSTMTVHAWRTQAE
VAAQTDMIFTVNSLMKDLVCETYNLNAFPLPSELETVLGLNMLWHRSRNTHPMLVWARNLFKQVVAEYTGKASIAPMHPP
MLTDDSGKSGKTGKGDAEKEDESRLSV
>Mature_347_residues
MVNVDTKLLVIFVELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPKALELGPKVREMLDLWAA
INEGDIATFDPLEAAGTFNISFAGTLGDALFDRFLLRVKRLAPGLQVRLTESSSWEADVAAMRSNELDLAFSPFPTRHPE
IVEEVVTSFNMWVCARKDHPVLKDHCSLDQYLECEHIFIAQGNPGTRAAPSLIPLDYALQQRGLKRHSTMTVHAWRTQAE
VAAQTDMIFTVNSLMKDLVCETYNLNAFPLPSELETVLGLNMLWHRSRNTHPMLVWARNLFKQVVAEYTGKASIAPMHPP
MLTDDSGKSGKTGKGDAEKEDESRLSV

Specific function: Regulates the transcription of one or more of the genes involved in virulence

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790147, Length=318, Percent_Identity=27.0440251572327, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081684, Length=270, Percent_Identity=23.7037037037037, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081766, Length=297, Percent_Identity=23.5690235690236, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PHCA_RALSO (P52698)

Other databases:

- EMBL:   L19269
- EMBL:   AF184046
- EMBL:   AF239238
- EMBL:   AL646052
- PIR:   A48655
- RefSeq:   NP_520869.1
- ProteinModelPortal:   P52698
- SMR:   P52698
- GeneID:   1221595
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2748
- NMPDR:   fig|267608.1.peg.2748
- HOGENOM:   HBG437118
- OMA:   HRSRNTH
- ProtClustDB:   CLSK897441
- BioCyc:   RSOL267608:RSC2748-MONOMER
- GO:   GO:0009405
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 38791; Mature: 38791

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVDTKLLVIFVELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPKALELGPKVREMLDLWAAINEGDIATFDPLEAAGTFNISFAGTLGDALFDRFLLRVKR
CCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LAPGLQVRLTESSSWEADVAAMRSNELDLAFSPFPTRHPEIVEEVVTSFNMWVCARKDHP
HCCCCEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEECCCCC
VLKDHCSLDQYLECEHIFIAQGNPGTRAAPSLIPLDYALQQRGLKRHSTMTVHAWRTQAE
CHHHHCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHCEEEEEEEHHHHH
VAAQTDMIFTVNSLMKDLVCETYNLNAFPLPSELETVLGLNMLWHRSRNTHPMLVWARNL
HHHHHHHEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FKQVVAEYTGKASIAPMHPPMLTDDSGKSGKTGKGDAEKEDESRLSV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MVNVDTKLLVIFVELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPKALELGPKVREMLDLWAAINEGDIATFDPLEAAGTFNISFAGTLGDALFDRFLLRVKR
CCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LAPGLQVRLTESSSWEADVAAMRSNELDLAFSPFPTRHPEIVEEVVTSFNMWVCARKDHP
HCCCCEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEECCCCC
VLKDHCSLDQYLECEHIFIAQGNPGTRAAPSLIPLDYALQQRGLKRHSTMTVHAWRTQAE
CHHHHCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHCEEEEEEEHHHHH
VAAQTDMIFTVNSLMKDLVCETYNLNAFPLPSELETVLGLNMLWHRSRNTHPMLVWARNL
HHHHHHHEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FKQVVAEYTGKASIAPMHPPMLTDDSGKSGKTGKGDAEKEDESRLSV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366033; 10913109; 11823852