Click here to switch to the map view.

The map label for this gene is gapA

Identifier: 17547468

GI number: 17547468

Start: 2959747

End: 2960745

Strand: Reverse

Name: gapA

Synonym: RSc2749

Alternate gene names: 17547468

Gene position: 2960745-2959747 (Counterclockwise)

Preceding gene: 17547469

Following gene: 17547467

Centisome position: 79.67

GC content: 64.06

Gene sequence:

>999_bases
ATGACCATCAAGATCGGCATCAACGGCTTCGGCCGCATCGGGCGCATGGTGTTCCGCGCCGCCATCGCCAACTTCAAGGA
CATCGAAGTCGTTGCCATCAACGACCTGCTCGAGCCCGACTACCTGGCGTACATGCTGAAGTACGACTCGGTGCACGGCC
GCTTCGACGGCGAAGTGTCGGTCGACGGCAATACGCTGGTCGTCAACGGCAAGAAGATCCGCCTGACCGCGGTCAAGGAT
CCGGCCGAACTGAAGTGGGGCGAGGTCGGCGCCGACGTGGTGGTCGAGTCGACCGGCATCTTCCTGACCAAGGAAGGCGC
GCAGAAGCACATCGACGCGGGCGCCAAGAAGGTGATCATGTCGGCGCCGTCCAAGGACGACACGCCGATGTTCGTCTACG
GCGTGAACCACGGCACCTACAAGGGCGAAGCGATCATCTCCAACGCCAGCTGCACCACCAACTGCCTGGCCCCGGTGGCC
AAGGTGCTGAACGACAAGTGGGGCATCAAGCGCGGCCTGATGACCACCGTGCACGCCGCCACCGCCACGCAGAAGACCGT
TGACGGCCCGTCCAACAAGGACTGGCGCGGCGGCCGCGGCATCCTGGAAAACATCATCCCGTCGTCGACCGGCGCCGCCA
AGGCCGTGGGCGTGGTGATCCCGCAGCTGAACAAGAAGCTGACGGGCATGTCGTTCCGCGTGCCGACCTCGGACGTGTCC
GTGGTCGACCTGACCGTCGAACTGGAAAAGGCGGCCAGCTACGACGAGATCTGCGCCGAAATGAAGGCCCAGAGCCAGGG
CGCCCTGAAGGGCGTGCTGGGCTACACGGAAGACAAGGTCGTGGCCACGGATTTCCGCGGCGATGCACGCACCTCGATCT
TCGACGCCGAAGCCGGCATCGCGCTGGACGGCACCTTCATCAAGGTCGTGAGCTGGTACGACAACGAGTGGGGCTACTCG
AACAAGGTGCTGGAAATGGCCCGCGTGGTGGCCAAGTAA

Upstream 100 bases:

>100_bases
AAGAGCGTCATCGCTCCGGCTGTCTGACAAGTTACCGCGCGGCACCCTTGAGGTGCCGCGTTGCCATCGTCGATTTCATT
TTGCTATCAGGAGACTGATC

Downstream 100 bases:

>100_bases
GCCTGCGCTTTTGCGCAAAGGGAAAACGCGCCTGCGGGCGCGTTTTTTCATGCCGCGCAAGGTTGGACCATCGGGGCGCC
GTGCAAGCAAAGTGATGCAG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PAELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKVLEMARVVAK

Sequences:

>Translated_332_residues
MTIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PAELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKVLEMARVVAK
>Mature_331_residues
TIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKDP
AELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVAK
VLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVSV
VDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYSN
KVLEMARVVAK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=60.6606606606607, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=56.838905775076, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=71.0843373493976, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1789295, Length=335, Percent_Identity=39.4029850746269, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=61.9047619047619, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=61.9047619047619, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32566163, Length=335, Percent_Identity=61.4925373134328, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17568413, Length=335, Percent_Identity=61.4925373134328, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=62.9518072289157, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=61.7469879518072, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=61.4457831325301, Blast_Score=412, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17933600, Length=327, Percent_Identity=61.1620795107034, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI18110149, Length=327, Percent_Identity=61.1620795107034, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI85725000, Length=327, Percent_Identity=60.8562691131498, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI22023983, Length=327, Percent_Identity=60.8562691131498, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=61.4197530864197, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_RALSO (P52694)

Other databases:

- EMBL:   AL646052
- EMBL:   L19269
- RefSeq:   NP_520870.1
- ProteinModelPortal:   P52694
- SMR:   P52694
- GeneID:   1221596
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2749
- NMPDR:   fig|267608.1.peg.2749
- HOGENOM:   HBG571736
- OMA:   DLKWDAI
- ProtClustDB:   CLSK897442
- BioCyc:   RSOL267608:RSC2749-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 35701; Mature: 35570

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 151-151 BINDING 35-35 BINDING 79-79 BINDING 181-181 BINDING 233-233 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPAELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HHCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEEHHCCCHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKVLEMARVVAK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
EEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPAELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HHCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEEHHCCCHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKVLEMARVVAK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852; 8366033