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The map label for this gene is miaA

Identifier: 17547283

GI number: 17547283

Start: 2773981

End: 2774952

Strand: Direct

Name: miaA

Synonym: RSc2564

Alternate gene names: 17547283

Gene position: 2773981-2774952 (Clockwise)

Preceding gene: 17547282

Following gene: 17547284

Centisome position: 74.64

GC content: 72.84

Gene sequence:

>972_bases
ATGACCGCCGCCCCGCACACCGCCCCGCGCGCCGTCTGCCTGCTCGGCCCGACCGCCTCCGGCAAGACCGCCGCCGCGCT
CGCGCTCGCCGAGCGCTGGCCGGTCGAGATCATCAGCATGGATTCCGCGCTGGTCTATCGCGACATGGACATCGGCACGG
CCAAGCCGAGCCGGGCGGAGCAGGCCATCGCGCCGCATCACCTGATCGACATCATCGACCCGCTCGATGCGTATTCCGCC
GCGCAGTTCGCCACCGACGCGCAGGCGCTGATCGAAGCCATCCGCGCGCGCGGCAGGCTGCCGCTGATCGTCGGCGGCAC
GATGCTGTATTACAAGGCGCTGACGCAGGGGCTGTCCGACCTGCCCGGCGCCGACCCGGCCATCCGCGCCGAGATCGACG
CCGAGGCCGCGCGCGACGGCTGGCCGGCGCTGCATGCGAAGCTCGCGCAGGTCGATCCCGTCACCGCGGCGCGGCTGCAT
GCCACCGATGCCCAGCGCATCCAGCGCGCACTGGAGCTGTACCGGCTGACCGGCCAGCCGATGTCCGCCCTGCTGGCGCG
CGAGGCCGGTGCCGCCGCCTTCCACCGGCACGAAGCCGCGGCCGCCTACCTGAGCATCGCGCTGGAGCCCGCCGACCGCG
CCGTGCTGCACGCCCGCATCGCACAGCGCTTCGATGCGATGCTGGCCGGCGGCCTGCTCGACGAGGTGGAGGCGCTGCGC
CGCCGGGGCGATCTGTCGCCGGTACTGCCGTCCATCCGCTGCGTCGGCTATCGCCAGGCGTGGGCCTACCTCGACGGCGA
GATCGACATGGCCACACTGCGCGAGCAGGGCATCGCCGCCACGCGCCAGCTCTGCAAGCGCCAGATCACATGGCTGCGCA
GCACGCCCGAGCGCCGCGTGGTCGACTGCCTCGCCCCCGACTACGTCGACCAGGTGGCACGCCTGGTCCGCAACGCACTC
GAAACGCCATGA

Upstream 100 bases:

>100_bases
ACCGAACGCGCCGACCAGTGCAACCACGGCCGGCCGACGTGGATCCAGCTGACGGTCGCCGATCTGGACCGGCTGTTCCT
GCGCGGCCAGTGACCGCACG

Downstream 100 bases:

>100_bases
CGACCACGCAAGCCGATCGCGCCACCTTCACCCTGCGCAGCGCCGCGCCGGACGACTGCGAGGCGCTGGTCCGCCTGATC
GGGGCGCTCGCCGAGTACGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTAAPHTAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAEQAIAPHHLIDIIDPLDAYSA
AQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSDLPGADPAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLH
ATDAQRIQRALELYRLTGQPMSALLAREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALR
RRGDLSPVLPSIRCVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRVVDCLAPDYVDQVARLVRNAL
ETP

Sequences:

>Translated_323_residues
MTAAPHTAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAEQAIAPHHLIDIIDPLDAYSA
AQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSDLPGADPAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLH
ATDAQRIQRALELYRLTGQPMSALLAREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALR
RRGDLSPVLPSIRCVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRVVDCLAPDYVDQVARLVRNAL
ETP
>Mature_322_residues
TAAPHTAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAEQAIAPHHLIDIIDPLDAYSAA
QFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSDLPGADPAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLHA
TDAQRIQRALELYRLTGQPMSALLAREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALRR
RGDLSPVLPSIRCVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRVVDCLAPDYVDQVARLVRNALE
TP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=34.4155844155844, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1790613, Length=309, Percent_Identity=52.4271844660194, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25144712, Length=275, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=301, Percent_Identity=27.5747508305648, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24649830, Length=348, Percent_Identity=29.8850574712644, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RALSO (Q8XWB0)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520685.1
- ProteinModelPortal:   Q8XWB0
- SMR:   Q8XWB0
- GeneID:   1221411
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2564
- NMPDR:   fig|267608.1.peg.2564
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   RSOL267608:RSC2564-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34967; Mature: 34835

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPHTAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAE
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCCHHH
QAIAPHHLIDIIDPLDAYSAAQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSD
HHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHH
LPGADPAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLHATDAQRIQRALELYRLTGQP
CCCCCCCEEEECCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
MSALLAREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALR
HHHHHHHHHCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RRGDLSPVLPSIRCVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRV
HCCCCCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VDCLAPDYVDQVARLVRNALETP
HHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAAPHTAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAE
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCCHHH
QAIAPHHLIDIIDPLDAYSAAQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSD
HHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHH
LPGADPAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLHATDAQRIQRALELYRLTGQP
CCCCCCCEEEECCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
MSALLAREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALR
HHHHHHHHHCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RRGDLSPVLPSIRCVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRV
HCCCCCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VDCLAPDYVDQVARLVRNALETP
HHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852