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The map label for this gene is ogt [H]

Identifier: 17547225

GI number: 17547225

Start: 2713098

End: 2713655

Strand: Direct

Name: ogt [H]

Synonym: RSc2506

Alternate gene names: 17547225

Gene position: 2713098-2713655 (Clockwise)

Preceding gene: 17547224

Following gene: 17547226

Centisome position: 73.0

GC content: 72.22

Gene sequence:

>558_bases
ATGACCGCCATGCTCCGCCTCACCTTCGCCTGCCCGCTCGGCGACCTGCTGCTGATCGCCACCGACACCCACCTGACCGG
CGCGTTCTTCACCGGGCAGAAGACGATCCCGGCCAACGCGCACCAGCTGCGCACGGGTGCCGACGCCGCCCTCCTCCTCG
AGGCCCAGGCACAGTTCGAAGCCTATTTCGCCGGCCGGCTACAGGTGTTCGACCTGCCGATGGCGCCCGCCGGCACGCCG
TTCCAGCACGACGTGTGGCGGCAGCTGTGCGCGATCCCCTTCGGCGAGCGCTGCACCTACGGCCAGATCACCGCCCGGCT
CGGACTCGCCCGTGAACATGCGCGCGCGGTCGGCACCGCCGTCGGACGCAACCCGATCTCGATCGCCATCCCGTGCCATC
GCGTGGTCGGCGCGGATGGCTCGCTGACCGGCTATGCCGGCGGACTACCGCGCAAGGCCGCGCTACTGCGGCTGGAAGGC
CATCCGGCACAGGTCGGCGCGCGGCTGCCGATGCCGGCCGATCCGCGCCAGGCCGCGCTGGCGCTCGCGCCTGCATGA

Upstream 100 bases:

>100_bases
CACGCCACGCCATGGCCACCCGGGCGGAAGACTGGGCGCCATGGCGCGCCTATGCCGCCATGCACCTGTGGCACCGCGAC
GATGCCCAGGCTCCGACCCC

Downstream 100 bases:

>100_bases
ATCGCGGCAAGCGCGGACAATGCTGCCTGCCGGCTCCGCACAGGGACACCGACCACCCGTCGGCCCCGTTCACACAACCA
TAACGAGACCACCATGCGCC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTAMLRLTFACPLGDLLLIATDTHLTGAFFTGQKTIPANAHQLRTGADAALLLEAQAQFEAYFAGRLQVFDLPMAPAGTP
FQHDVWRQLCAIPFGERCTYGQITARLGLAREHARAVGTAVGRNPISIAIPCHRVVGADGSLTGYAGGLPRKAALLRLEG
HPAQVGARLPMPADPRQAALALAPA

Sequences:

>Translated_185_residues
MTAMLRLTFACPLGDLLLIATDTHLTGAFFTGQKTIPANAHQLRTGADAALLLEAQAQFEAYFAGRLQVFDLPMAPAGTP
FQHDVWRQLCAIPFGERCTYGQITARLGLAREHARAVGTAVGRNPISIAIPCHRVVGADGSLTGYAGGLPRKAALLRLEG
HPAQVGARLPMPADPRQAALALAPA
>Mature_184_residues
TAMLRLTFACPLGDLLLIATDTHLTGAFFTGQKTIPANAHQLRTGADAALLLEAQAQFEAYFAGRLQVFDLPMAPAGTPF
QHDVWRQLCAIPFGERCTYGQITARLGLAREHARAVGTAVGRNPISIAIPCHRVVGADGSLTGYAGGLPRKAALLRLEGH
PAQVGARLPMPADPRQAALALAPA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=35.672514619883, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=54.2857142857143, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1788542, Length=93, Percent_Identity=45.1612903225806, Blast_Score=82, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=92, Percent_Identity=40.2173913043478, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19529; Mature: 19398

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMLRLTFACPLGDLLLIATDTHLTGAFFTGQKTIPANAHQLRTGADAALLLEAQAQFE
CCEEEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEEEEEEHHHHEE
AYFAGRLQVFDLPMAPAGTPFQHDVWRQLCAIPFGERCTYGQITARLGLAREHARAVGTA
EEEECEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGRNPISIAIPCHRVVGADGSLTGYAGGLPRKAALLRLEGHPAQVGARLPMPADPRQAAL
CCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCCCHHEE
ALAPA
EECCC
>Mature Secondary Structure 
TAMLRLTFACPLGDLLLIATDTHLTGAFFTGQKTIPANAHQLRTGADAALLLEAQAQFE
CEEEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEEEEEEHHHHEE
AYFAGRLQVFDLPMAPAGTPFQHDVWRQLCAIPFGERCTYGQITARLGLAREHARAVGTA
EEEECEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGRNPISIAIPCHRVVGADGSLTGYAGGLPRKAALLRLEGHPAQVGARLPMPADPRQAAL
CCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCCCHHEE
ALAPA
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA