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The map label for this gene is cysH [H]

Identifier: 17547142

GI number: 17547142

Start: 2627596

End: 2628399

Strand: Reverse

Name: cysH [H]

Synonym: RSc2423

Alternate gene names: 17547142

Gene position: 2628399-2627596 (Counterclockwise)

Preceding gene: 17547143

Following gene: 17547141

Centisome position: 70.72

GC content: 68.16

Gene sequence:

>804_bases
ATGAGCGCGCTCCAAGAGGCCGCCGTGTCGGAAGTGCCGATGTCCGCGATCGGGCGCCCGGTGCTGTGGTCGCGTCCGGT
CTACACGGGCACGCCCGAGGGGCTGGCCGTCAAGGAGGCCGCGCTGCTCGAGCGCCTGACCGAGATTGCCGCCAAGCATG
GCACCGCGAAGTTCGCCAGCAGCCTGGCCGCCGAGGACATGGTGGTGACGGACGCCATTCTGCGCAGTCCGGAAGCCGTG
CGCGCGCATCTGCCGATCTTCACGCTGCAGACCGGCCGCCTGCACGCCGAGACGCTGGCCATGCTCGAGCGCATCCGCGA
ACACTACGGCTATGCGATCGAGCAGTTCGCGCCGGATGCCCGCGCGGTGCAAGCCTATGTGCGCGACCACGGGCTGAACG
CGTTCTACGACAGCATCGAATTGCGCAAGGCCTGCTGCACCATCCGCAAGGTGGTGCCGCTGAACCGCGCGCTCCGGGAC
GCAGATGCATGGCTGAGCGGCCAGCGCCGCGAGCAGGCCGTCACCCGTGCCGACCTGCCGTTCGTCGAAGAGGACGACAC
GCGCGGCATCGCCAAATACAATCCGCTGTTCGACTGGACCGAGGCCGAAGTCTGGGCCTACCTGGAACGGCATGCGGTGC
CCGTCAACGCGCTGCACGATAAGGGCTACCCCAGCATCGGCTGCGAGCCCTGCACGCGTGCGGTGCGCGCCGGCGAGGAC
GTGCGCGCCGGCCGCTGGTGGTGGGAATCGCGCGATAGCAAGGAATGCGGTTTGCATGCGACGAACCTGTCGCACAAGAG
CTAA

Upstream 100 bases:

>100_bases
CGCTCAACGGCTTCGGCGAGATCAGCGTGCGCTACCAGGGCGCCATCGACGAACCGCGCCCGCTGTTCCGCCGCGAGCGC
GCCTCCACGGTGAGCGCAGC

Downstream 100 bases:

>100_bases
GGATAGGAAGATGGGAGTGCTGAACGAAATCCCCGGCACGGCGCTGACGCCGATGCAGAACGACCACCTCGACTGGCTGG
AAGCCGAGTCGATCTACATC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSALQEAAVSEVPMSAIGRPVLWSRPVYTGTPEGLAVKEAALLERLTEIAAKHGTAKFASSLAAEDMVVTDAILRSPEAV
RAHLPIFTLQTGRLHAETLAMLERIREHYGYAIEQFAPDARAVQAYVRDHGLNAFYDSIELRKACCTIRKVVPLNRALRD
ADAWLSGQRREQAVTRADLPFVEEDDTRGIAKYNPLFDWTEAEVWAYLERHAVPVNALHDKGYPSIGCEPCTRAVRAGED
VRAGRWWWESRDSKECGLHATNLSHKS

Sequences:

>Translated_267_residues
MSALQEAAVSEVPMSAIGRPVLWSRPVYTGTPEGLAVKEAALLERLTEIAAKHGTAKFASSLAAEDMVVTDAILRSPEAV
RAHLPIFTLQTGRLHAETLAMLERIREHYGYAIEQFAPDARAVQAYVRDHGLNAFYDSIELRKACCTIRKVVPLNRALRD
ADAWLSGQRREQAVTRADLPFVEEDDTRGIAKYNPLFDWTEAEVWAYLERHAVPVNALHDKGYPSIGCEPCTRAVRAGED
VRAGRWWWESRDSKECGLHATNLSHKS
>Mature_266_residues
SALQEAAVSEVPMSAIGRPVLWSRPVYTGTPEGLAVKEAALLERLTEIAAKHGTAKFASSLAAEDMVVTDAILRSPEAVR
AHLPIFTLQTGRLHAETLAMLERIREHYGYAIEQFAPDARAVQAYVRDHGLNAFYDSIELRKACCTIRKVVPLNRALRDA
DAWLSGQRREQAVTRADLPFVEEDDTRGIAKYNPLFDWTEAEVWAYLERHAVPVNALHDKGYPSIGCEPCTRAVRAGEDV
RAGRWWWESRDSKECGLHATNLSHKS

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=182, Percent_Identity=30.2197802197802, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325425, Length=112, Percent_Identity=41.9642857142857, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29806; Mature: 29675

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALQEAAVSEVPMSAIGRPVLWSRPVYTGTPEGLAVKEAALLERLTEIAAKHGTAKFAS
CCHHHHHHHHHCCHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
SLAAEDMVVTDAILRSPEAVRAHLPIFTLQTGRLHAETLAMLERIREHYGYAIEQFAPDA
HHHHHHHHHHHHHHHCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHH
RAVQAYVRDHGLNAFYDSIELRKACCTIRKVVPLNRALRDADAWLSGQRREQAVTRADLP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
FVEEDDTRGIAKYNPLFDWTEAEVWAYLERHAVPVNALHDKGYPSIGCEPCTRAVRAGED
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCC
VRAGRWWWESRDSKECGLHATNLSHKS
CCCCCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
SALQEAAVSEVPMSAIGRPVLWSRPVYTGTPEGLAVKEAALLERLTEIAAKHGTAKFAS
CHHHHHHHHHCCHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
SLAAEDMVVTDAILRSPEAVRAHLPIFTLQTGRLHAETLAMLERIREHYGYAIEQFAPDA
HHHHHHHHHHHHHHHCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHH
RAVQAYVRDHGLNAFYDSIELRKACCTIRKVVPLNRALRDADAWLSGQRREQAVTRADLP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
FVEEDDTRGIAKYNPLFDWTEAEVWAYLERHAVPVNALHDKGYPSIGCEPCTRAVRAGED
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCC
VRAGRWWWESRDSKECGLHATNLSHKS
CCCCCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]