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The map label for this gene is cysD [H]

Identifier: 17547141

GI number: 17547141

Start: 2626635

End: 2627585

Strand: Reverse

Name: cysD [H]

Synonym: RSc2422

Alternate gene names: 17547141

Gene position: 2627585-2626635 (Counterclockwise)

Preceding gene: 17547142

Following gene: 17547140

Centisome position: 70.7

GC content: 66.98

Gene sequence:

>951_bases
ATGGGAGTGCTGAACGAAATCCCCGGCACGGCGCTGACGCCGATGCAGAACGACCACCTCGACTGGCTGGAAGCCGAGTC
GATCTACATCATCCGCGAGGTGGTGGCCGAGTGCCGCAACCCGGCGCTGCTGTTCTCCGGCGGCAAGGACTCGATCGTCA
TGCTGCACCTGGCGCTCAAGGCCTTCCGCCTGGGCGATCGCAAGATCGAGCTGCCGTTCCCGCTGGTGCACATCGATACG
GGCCACAACTACCCGGAAGTGATCGCCTTCCGTGACCAGCGTGTCGCCGAACTGGGCGCGCGCCTGGTGGTCGGCCACGT
GGAAGATTCCATCAAGCGCGGCACCGTGCGCCTGCGTCGTGAAACCGACTCGCGCAACGCCGCGCAGGCCGTCACGCTGC
TGGAGACCATCGAGTCGCACGGCTTCGACGCGCTGATGGGCGGCGCCCGCCGCGACGAAGAGAAGGCCCGCGCCAAGGAG
CGCATCTTCTCGTTCCGCGACGAGTTCGGCCAGTGGGACCCGAAGGCCCAGCGCCCGGAGCTCTGGAGCCTGTACAACGC
CCGCATGGCACGGGGCGAGCAGATGCGCGTGTTCCCGATCTCCAACTGGACGGAGCTCGACGTGTGGCAGTACATCGCCC
GCGAGAACCTGGCGCTGCCGCCGATCTACTACGCGCATCAGCGTGAAGTCGTGCGCAAGAACGGCCTGCTGGTGCCGGTC
ACGCCCATCACGCCCAAGGCCGACGGCGACGTGAGCGAAGTCCTGTCGGTGCGCTTCCGCACCGTCGGCGACATCAGCTG
CACCTGCCCGGTGGCAAGCACCGCCGCCACGCCGGCCGAGATCATCGCCGAGACGGCCGTGACCGAGATCACCGAACGCG
GCGCCACGCGCATGGACGACCAGACCAGCGAGGCGTCGATGGAAAAGCGCAAGAAGGAAGGGTATTTCTGA

Upstream 100 bases:

>100_bases
GAGGACGTGCGCGCCGGCCGCTGGTGGTGGGAATCGCGCGATAGCAAGGAATGCGGTTTGCATGCGACGAACCTGTCGCA
CAAGAGCTAAGGATAGGAAG

Downstream 100 bases:

>100_bases
TCATGACGAACCAAGCATCCCATCAGGGCCTGTTGCGCTTCATCACCGCCGGTTCGGTCGACGACGGCAAGAGCACGCTG
ATCGGTCGTCTGCTGTACGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MGVLNEIPGTALTPMQNDHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPLVHIDT
GHNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKE
RIFSFRDEFGQWDPKAQRPELWSLYNARMARGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHQREVVRKNGLLVPV
TPITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF

Sequences:

>Translated_316_residues
MGVLNEIPGTALTPMQNDHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPLVHIDT
GHNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKE
RIFSFRDEFGQWDPKAQRPELWSLYNARMARGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHQREVVRKNGLLVPV
TPITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF
>Mature_315_residues
GVLNEIPGTALTPMQNDHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPLVHIDTG
HNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKER
IFSFRDEFGQWDPKAQRPELWSLYNARMARGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHQREVVRKNGLLVPVT
PITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=46.8646864686469, Blast_Score=258, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35633; Mature: 35502

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLNEIPGTALTPMQNDHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALK
CCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
AFRLGDRKIELPFPLVHIDTGHNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRR
HHHCCCEEEECCCCEEEEECCCCCCCEEEECHHHHHHHCHHEEEEHHHHHHHHCCEEEEE
ETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPE
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LWSLYNARMARGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHQREVVRKNGLLVPV
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEE
TPITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDD
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QTSEASMEKRKKEGYF
CHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVLNEIPGTALTPMQNDHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALK
CCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
AFRLGDRKIELPFPLVHIDTGHNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRR
HHHCCCEEEECCCCEEEEECCCCCCCEEEECHHHHHHHCHHEEEEHHHHHHHHCCEEEEE
ETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPE
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LWSLYNARMARGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHQREVVRKNGLLVPV
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEE
TPITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDD
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QTSEASMEKRKKEGYF
CHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]