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The map label for this gene is ilvD

Identifier: 17547131

GI number: 17547131

Start: 2616780

End: 2618453

Strand: Direct

Name: ilvD

Synonym: RSc2412

Alternate gene names: 17547131

Gene position: 2616780-2618453 (Clockwise)

Preceding gene: 17547126

Following gene: 17547135

Centisome position: 70.41

GC content: 67.14

Gene sequence:

>1674_bases
ATGCCAGACAACAAGCGTTCCCAGCACATCACCCAGGGTGTGGCGCGCTCGCCCAACCGCTCGATGTACTACGCGCTGGG
CTACAAGAAGGAAGACTTCAGCAACCCGATGATCGGCGTAGCCAATGGTCATTCGACCATCACGCCGTGCAACTCGGGCC
TGCAGAAGCTGGCTGACGCGGCCGTGGCCGCCGTCAAGGCGTCGAACGCCAATCCGCAGATCTTCGGCACGCCCACCATT
TCCGACGGCATGTCGATGGGCACCGAGGGCATGAAGTATTCGCTGATCTCGCGCGAGGTCATCGCCGACTGCATCGAGAC
CTGCGCACAGGGCCAGTGGATGGACGGCGTGGTCGTTATCGGCGGCTGCGACAAGAACATGCCCGGCGGCATGATCGCCC
TGGCCCGCACCAACGTGCCCGGCATCTACGTGTACGGCGGCACCATCAAACCGGGCAACTGGAAGGGCAAGGATCTGACC
ATCGTGTCGTCGTTCGAGGCTGTGGGCGAGTTCACCGCCGGCCGCATGAGCGAGGAAGACTTCGAGGGCGTGGAGCGCAA
CGCCTGCCCGACCTCGGGTTCGTGCGGCGGCATGTACACCGCCAACACGATGAGCTCGTCGTTCGAGGCGCTGGGCATGT
CGCTGCTGTATTCGTCGACCATGGCCAACCCCGATCAGGAGAAGGTCGACAGCGCCGCCGAATCGGCCCGCGTGCTGGTC
GAGGCCGTGCGCCGCGACCTCAAGCCGCGCGACATCATCACGCGCAAGTCCATCGAGAACGCCGTGGCCGTCATCATGGC
CACCGGCGGCTCGACCAACGCCGTGCTGCACTACCTGGCCATCGCCCACGCCGCCGGCGTGGAATGGACCATTGACGACT
TCGAGCGCGTGCGTCAGCGCGTGCCGGTCATCTGCAACCTGAAGCCGTCGGGCCAGTACGTGGCCACCGACCTGCACAAG
GCCGGCGGCATCCCGCAGGTGATGAAGATCCTCCTGAACGCTGGCCTGCTGCACGGCGACTGCGTCACCATCACCGGCAA
GACGCTGGCCGAAGAGCTGGCCAACGTGCCCGACCAACCGCGCGCGGACCAGGACGTGATCCTGCCGATCGAGCGCGCGC
TCTACCAGCAGGGCCACCTCGCCATCCTCAAGGGCAACCTGGCCGAAGAAGGCGCCGTGGCGAAGATCACCGGCCTGAAG
AACCCGGTCATCTCCGGCCCGGCGCGCGTATTCGACGACGAGCAGAGCGCGATGACCGCCATCCTGGCCGACCAGATCAA
GGCCGGCGACGTGCTGGTGCTGCGCTACCTGGGCCCGAAGGGCGGCCCCGGCATGCCGGAAATGCTGGCGCCGACCTCGG
CCATCATCGGCAAGGGCCTGGGCGAATCGGTGGGCTTCGTCACCGACGGGCGCTTCTCGGGCGGCACCTGGGGCATGGTG
GTCGGCCACGTCGCGCCGGAAGCCTTCGTGGGCGGCACCATCGCCCTGGTGCAGGAAGGTGATTCGATCACCATCGACGC
GCACCAGCGCCTGCTGCAGCTGAACGTGCCGGAAGACGAACTGGCCCGCCGCCGCGCCGCGTGGAAGCAGCCGGCACCGC
GCTACACGCGCGGCGTGCTCGCCAAGTTCGCGCAGCTGACGTCGACGGCCAGCAAGGGCGCCGTCACGGACTGA

Upstream 100 bases:

>100_bases
ATAATCGCTCAATAATATATTTGACACGACTTTTGTCCAGCGGCATGCTGTGCGCCAGCGATTTTCCCAAAACGAAATGT
CCGACCCCACGGAGCCTCCC

Downstream 100 bases:

>100_bases
CCCCGCGCCGTTGCCGCGCTCCAAAACGAAAAACCCGCCATCCGGCGGGTTTTTTCATGGGCTCGCGATCCGTGCCGACG
CTAGCGCGGCGGGGTCCTGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MPDNKRSQHITQGVARSPNRSMYYALGYKKEDFSNPMIGVANGHSTITPCNSGLQKLADAAVAAVKASNANPQIFGTPTI
SDGMSMGTEGMKYSLISREVIADCIETCAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLT
IVSSFEAVGEFTAGRMSEEDFEGVERNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVRRDLKPRDIITRKSIENAVAVIMATGGSTNAVLHYLAIAHAAGVEWTIDDFERVRQRVPVICNLKPSGQYVATDLHK
AGGIPQVMKILLNAGLLHGDCVTITGKTLAEELANVPDQPRADQDVILPIERALYQQGHLAILKGNLAEEGAVAKITGLK
NPVISGPARVFDDEQSAMTAILADQIKAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFVTDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHQRLLQLNVPEDELARRRAAWKQPAPRYTRGVLAKFAQLTSTASKGAVTD

Sequences:

>Translated_557_residues
MPDNKRSQHITQGVARSPNRSMYYALGYKKEDFSNPMIGVANGHSTITPCNSGLQKLADAAVAAVKASNANPQIFGTPTI
SDGMSMGTEGMKYSLISREVIADCIETCAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLT
IVSSFEAVGEFTAGRMSEEDFEGVERNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVRRDLKPRDIITRKSIENAVAVIMATGGSTNAVLHYLAIAHAAGVEWTIDDFERVRQRVPVICNLKPSGQYVATDLHK
AGGIPQVMKILLNAGLLHGDCVTITGKTLAEELANVPDQPRADQDVILPIERALYQQGHLAILKGNLAEEGAVAKITGLK
NPVISGPARVFDDEQSAMTAILADQIKAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFVTDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHQRLLQLNVPEDELARRRAAWKQPAPRYTRGVLAKFAQLTSTASKGAVTD
>Mature_556_residues
PDNKRSQHITQGVARSPNRSMYYALGYKKEDFSNPMIGVANGHSTITPCNSGLQKLADAAVAAVKASNANPQIFGTPTIS
DGMSMGTEGMKYSLISREVIADCIETCAQGQWMDGVVVIGGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTI
VSSFEAVGEFTAGRMSEEDFEGVERNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLVE
AVRRDLKPRDIITRKSIENAVAVIMATGGSTNAVLHYLAIAHAAGVEWTIDDFERVRQRVPVICNLKPSGQYVATDLHKA
GGIPQVMKILLNAGLLHGDCVTITGKTLAEELANVPDQPRADQDVILPIERALYQQGHLAILKGNLAEEGAVAKITGLKN
PVISGPARVFDDEQSAMTAILADQIKAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFVTDGRFSGGTWGMVV
GHVAPEAFVGGTIALVQEGDSITIDAHQRLLQLNVPEDELARRRAAWKQPAPRYTRGVLAKFAQLTSTASKGAVTD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=36.4686468646865, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI2367371, Length=487, Percent_Identity=34.2915811088296, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1788157, Length=482, Percent_Identity=32.5726141078838, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786464, Length=497, Percent_Identity=35.010060362173, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322476, Length=562, Percent_Identity=45.5516014234875, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_RALSO (Q8XWR1)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520533.1
- ProteinModelPortal:   Q8XWR1
- SMR:   Q8XWR1
- GeneID:   1221258
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2412
- NMPDR:   fig|267608.1.peg.2412
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- ProtClustDB:   PRK00911
- BioCyc:   RSOL267608:RSC2412-MONOMER
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58966; Mature: 58835

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNKRSQHITQGVARSPNRSMYYALGYKKEDFSNPMIGVANGHSTITPCNSGLQKLADA
CCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCEEEEECCCCEECCHHHHHHHHHHH
AVAAVKASNANPQIFGTPTISDGMSMGTEGMKYSLISREVIADCIETCAQGQWMDGVVVI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSEED
ECCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHCCCCCCHH
FEGVERNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVRRDLKPRDIITRKSIENAVAVIMATGGSTNAVLHYLAIAHAAGVEWTIDDFERVRQR
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
VPVICNLKPSGQYVATDLHKAGGIPQVMKILLNAGLLHGDCVTITGKTLAEELANVPDQP
CCEEEEECCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCC
RADQDVILPIERALYQQGHLAILKGNLAEEGAVAKITGLKNPVISGPARVFDDEQSAMTA
CCCCCEEEHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHH
ILADQIKAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFVTDGRFSGGTWGMV
HHHHHCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHQRLLQLNVPEDELARRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
AKFAQLTSTASKGAVTD
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PDNKRSQHITQGVARSPNRSMYYALGYKKEDFSNPMIGVANGHSTITPCNSGLQKLADA
CCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCEEEEECCCCEECCHHHHHHHHHHH
AVAAVKASNANPQIFGTPTISDGMSMGTEGMKYSLISREVIADCIETCAQGQWMDGVVVI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSEED
ECCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHCCCCCCHH
FEGVERNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVRRDLKPRDIITRKSIENAVAVIMATGGSTNAVLHYLAIAHAAGVEWTIDDFERVRQR
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
VPVICNLKPSGQYVATDLHKAGGIPQVMKILLNAGLLHGDCVTITGKTLAEELANVPDQP
CCEEEEECCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCC
RADQDVILPIERALYQQGHLAILKGNLAEEGAVAKITGLKNPVISGPARVFDDEQSAMTA
CCCCCEEEHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHH
ILADQIKAGDVLVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFVTDGRFSGGTWGMV
HHHHHCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHQRLLQLNVPEDELARRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
AKFAQLTSTASKGAVTD
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852