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The map label for this gene is puuB [H]

Identifier: 17546832

GI number: 17546832

Start: 2288569

End: 2289732

Strand: Reverse

Name: puuB [H]

Synonym: RSc2113

Alternate gene names: 17546832

Gene position: 2289732-2288569 (Counterclockwise)

Preceding gene: 17546834

Following gene: 17546831

Centisome position: 61.61

GC content: 60.22

Gene sequence:

>1164_bases
TTGGCGCTCAATTTTCCGGACGATGAGGTGGTGCTGGTGGACGCCCAGGAAGTGGGTTTCGGCGCGTCGGGAAGAAATGC
CGGTTTTGCAATCGACCTGCCTCACGATATCGGGGCGGAAGACTATATCGGCGACATTGAAACCGCAAAAATCAGTTTGA
ACCTGAACTTGACCGGACAGCGGATCCTCAAGTCCCTGGTTGACGCGCACCAGATTGATTGCCAGTTCCGGGCCTCCGGA
AAGTATCAGGCGGCGGTTGAAGACCGGGGCGTGGCGGTACTCGAAGCCTACCGTCGCGGCCTCGACAAGCTTGGCCAGGC
GTACGAAATGATTGACGGCAAGGATTTGCCCCATCACATCGGCACGTCGTTCTACCGGCAGGCACTGTTCACGCCGGGAA
CCGCCCTGATCCAGCCTTCTGCGCTGGTGAAGGGGCTGGCCGACAGTCTGCCCCGGAATGTCAGCCTGTATGAGCGCACC
GCCATTGCCGATGTCGAGTACGGTGAAAAAATCGTGCTCTCCCATGCGCATGGCCGCATTGCCGCGGACAAGCTGATTCT
CACGAACAATGCCTTTGGCATGCGCTTCGGCTTTCTGCGCGGGACGATGCTTCCGATTTTCACCTATGCGAGCCTCACGA
GGCCGCTGACGCCGGCAGAGCAGGCGTGTCTTGGCGGGAAACCGTTCTGGGGCCTCATTCCGGCGGACCCGTTCGGGACC
ACGTTGCGCCGCACGCATGACAACCGCATCCTGGTCAGAAACAGCTTCAGCTTCAATCGCGACGGGCGCGGCAAGCAGAA
GTATCTCGACCGCTTTGTCAGGACACACAGGCATGCCTTCGAGAGGCGATTTCCGATGCTGCCAGACGTCGAGTTCGAAT
ACACATGGGGCGGCTCGCTGGCGCTGTCACGCAATCATATGGGCCATTTCGGCCCGCTCGCGCCCCATGTTTACGGCGCA
CTTTGCTGCAACGGTCTGGGCGTCACCCGGGGCACGGTCACCGGCACGTTGCTGGCGGATTACCTGGCGGGCAAGCGCAG
CGATCTGATTGATTTCCTGCTGTCGTCACCCGGCCCGAACAGGAATCCGCCGGAACCGATTCTTTCAATTGGCGTGAATG
CCAATCTTTGGCTGGGACAACACCGTGCCGGCGCTGAAGTCTAG

Upstream 100 bases:

>100_bases
GAGTCAGCCCCGCCGGCCAAAACCATCTCATTCCGGAACAACGAATGCGCGCTGGGCCGTCGTTGGCGCCGGTTTTACCG
GCCTGGCTGCTGCGCGGCAA

Downstream 100 bases:

>100_bases
AACTTAAAACATCCTATCCAAGGAAAGCGTATCGAACATCAATAGGTATTGCCGAATGCCATAAACCGATTTCATTGATT
AGACCCGGATTCATCATTCC

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MALNFPDDEVVLVDAQEVGFGASGRNAGFAIDLPHDIGAEDYIGDIETAKISLNLNLTGQRILKSLVDAHQIDCQFRASG
KYQAAVEDRGVAVLEAYRRGLDKLGQAYEMIDGKDLPHHIGTSFYRQALFTPGTALIQPSALVKGLADSLPRNVSLYERT
AIADVEYGEKIVLSHAHGRIAADKLILTNNAFGMRFGFLRGTMLPIFTYASLTRPLTPAEQACLGGKPFWGLIPADPFGT
TLRRTHDNRILVRNSFSFNRDGRGKQKYLDRFVRTHRHAFERRFPMLPDVEFEYTWGGSLALSRNHMGHFGPLAPHVYGA
LCCNGLGVTRGTVTGTLLADYLAGKRSDLIDFLLSSPGPNRNPPEPILSIGVNANLWLGQHRAGAEV

Sequences:

>Translated_387_residues
MALNFPDDEVVLVDAQEVGFGASGRNAGFAIDLPHDIGAEDYIGDIETAKISLNLNLTGQRILKSLVDAHQIDCQFRASG
KYQAAVEDRGVAVLEAYRRGLDKLGQAYEMIDGKDLPHHIGTSFYRQALFTPGTALIQPSALVKGLADSLPRNVSLYERT
AIADVEYGEKIVLSHAHGRIAADKLILTNNAFGMRFGFLRGTMLPIFTYASLTRPLTPAEQACLGGKPFWGLIPADPFGT
TLRRTHDNRILVRNSFSFNRDGRGKQKYLDRFVRTHRHAFERRFPMLPDVEFEYTWGGSLALSRNHMGHFGPLAPHVYGA
LCCNGLGVTRGTVTGTLLADYLAGKRSDLIDFLLSSPGPNRNPPEPILSIGVNANLWLGQHRAGAEV
>Mature_386_residues
ALNFPDDEVVLVDAQEVGFGASGRNAGFAIDLPHDIGAEDYIGDIETAKISLNLNLTGQRILKSLVDAHQIDCQFRASGK
YQAAVEDRGVAVLEAYRRGLDKLGQAYEMIDGKDLPHHIGTSFYRQALFTPGTALIQPSALVKGLADSLPRNVSLYERTA
IADVEYGEKIVLSHAHGRIAADKLILTNNAFGMRFGFLRGTMLPIFTYASLTRPLTPAEQACLGGKPFWGLIPADPFGTT
LRRTHDNRILVRNSFSFNRDGRGKQKYLDRFVRTHRHAFERRFPMLPDVEFEYTWGGSLALSRNHMGHFGPLAPHVYGAL
CCNGLGVTRGTVTGTLLADYLAGKRSDLIDFLLSSPGPNRNPPEPILSIGVNANLWLGQHRAGAEV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=372, Percent_Identity=26.8817204301075, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42471; Mature: 42340

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNFPDDEVVLVDAQEVGFGASGRNAGFAIDLPHDIGAEDYIGDIETAKISLNLNLTGQ
CCCCCCCCCEEEEEHHHCCCCCCCCCCCEEEECCCCCCCHHHCCCCEEEEEEEEEECCHH
RILKSLVDAHQIDCQFRASGKYQAAVEDRGVAVLEAYRRGLDKLGQAYEMIDGKDLPHHI
HHHHHHHHHHHCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GTSFYRQALFTPGTALIQPSALVKGLADSLPRNVSLYERTAIADVEYGEKIVLSHAHGRI
HHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCEEEEEECCCCE
AADKLILTNNAFGMRFGFLRGTMLPIFTYASLTRPLTPAEQACLGGKPFWGLIPADPFGT
EECEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCH
TLRRTHDNRILVRNSFSFNRDGRGKQKYLDRFVRTHRHAFERRFPMLPDVEFEYTWGGSL
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEE
ALSRNHMGHFGPLAPHVYGALCCNGLGVTRGTVTGTLLADYLAGKRSDLIDFLLSSPGPN
EEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
RNPPEPILSIGVNANLWLGQHRAGAEV
CCCCCCCEEECCCCEEEECCCCCCCCC
>Mature Secondary Structure 
ALNFPDDEVVLVDAQEVGFGASGRNAGFAIDLPHDIGAEDYIGDIETAKISLNLNLTGQ
CCCCCCCCEEEEEHHHCCCCCCCCCCCEEEECCCCCCCHHHCCCCEEEEEEEEEECCHH
RILKSLVDAHQIDCQFRASGKYQAAVEDRGVAVLEAYRRGLDKLGQAYEMIDGKDLPHHI
HHHHHHHHHHHCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GTSFYRQALFTPGTALIQPSALVKGLADSLPRNVSLYERTAIADVEYGEKIVLSHAHGRI
HHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCEEEEEECCCCE
AADKLILTNNAFGMRFGFLRGTMLPIFTYASLTRPLTPAEQACLGGKPFWGLIPADPFGT
EECEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCH
TLRRTHDNRILVRNSFSFNRDGRGKQKYLDRFVRTHRHAFERRFPMLPDVEFEYTWGGSL
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEE
ALSRNHMGHFGPLAPHVYGALCCNGLGVTRGTVTGTLLADYLAGKRSDLIDFLLSSPGPN
EEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
RNPPEPILSIGVNANLWLGQHRAGAEV
CCCCCCCEEECCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]