Click here to switch to the map view.

The map label for this gene is nuoB [H]

Identifier: 17546780

GI number: 17546780

Start: 2235087

End: 2235569

Strand: Reverse

Name: nuoB [H]

Synonym: RSc2061

Alternate gene names: 17546780

Gene position: 2235569-2235087 (Counterclockwise)

Preceding gene: 17546781

Following gene: 17546779

Centisome position: 60.15

GC content: 64.6

Gene sequence:

>483_bases
ATGGCGATCGAAGGCGTACTCAACGAAGGCTTTGTCACCACCACCGCTGACAAGCTGATCAACTGGACCCGCACCGGGTC
ACTGTGGCCGATGACGTTCGGCCTGGCCTGCTGCGCGGTGGAAATGATGCACGCTGGCGCATCGCGTTATGACCTGGACC
GTTTCGGCGTGGTGTTCCGCCCGTCGCCGCGCCAGTCCGACGTGATGATCGTGGCCGGCACGCTGTGCAACAAGATGGCG
CCCGCGCTGCGCAAGGTCTACGACCAGATGGCCGAGCCGCGCTGGGTCATTTCGATGGGCTCGTGTGCCAACGGCGGCGG
CTACTACCACTATTCGTACTCCGTCGTGCGCGGCTGCGATCGCATCGTGCCGGTTGACGTGTACGTGCCGGGCTGCCCGC
CGACAGCCGAAGCGCTGATCTACGGCATCATCCAGCTGCAGGCGAAGATCCGCCGTACCAACACCATCGCGCGCAAGGGC
TGA

Upstream 100 bases:

>100_bases
CTGGCCGGGTTTCTTCGCGATGGGCGTGTTTCTTCTCGAGTTTCTTGTGGGCTTCGTCTACATCTGGAAAAAAGGCGCGC
TCGATTGGGAGTGATGTGAA

Downstream 100 bases:

>100_bases
CATGACCGACAAGCTTGCCACCCTGCAGTCCGCGCTCGAAAAGGCGCTGGGCAATCGCATCCAGAGCCTGACCGAGGCGG
TCGGTGAAATCACGCTCGTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQAKIRRTNTIARKG

Sequences:

>Translated_160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQAKIRRTNTIARKG
>Mature_159_residues
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQAKIRRTNTIARKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=80.1369863013699, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=50.3355704697987, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=140, Percent_Identity=37.1428571428571, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1788834, Length=111, Percent_Identity=40.5405405405405, Blast_Score=90, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=136, Percent_Identity=73.5294117647059, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=80.7142857142857, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=80.7142857142857, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=81.4285714285714, Blast_Score=253, Evalue=3e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17604; Mature: 17473

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGASRYDLDRFGVVFR
CCCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQAKIRRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGASRYDLDRFGVVFR
CCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQAKIRRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA