The gene/protein map for NC_003295 is currently unavailable.
Definition Ralstonia solanacearum GMI1000, complete genome.
Accession NC_003295
Length 3,716,413

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The map label for this gene is braF [H]

Identifier: 17546757

GI number: 17546757

Start: 2210808

End: 2211671

Strand: Reverse

Name: braF [H]

Synonym: RSc2038

Alternate gene names: 17546757

Gene position: 2211671-2210808 (Counterclockwise)

Preceding gene: 17546758

Following gene: 17546756

Centisome position: 59.51

GC content: 70.02

Gene sequence:

>864_bases
ATGAGGCCCTTCACCGCACGCCCCGACCGCGCGCAGGTCGGAAACTTCAGCGGCACCACCACCGGCATGGGCCACGTGCT
CGAGCCGGACGCCATCGACGTCTCGCACGGCCCCATCCTGTACCTCGAAGACGTGACGGTGCGCTTCGGCGGCTTCCGCG
CGCTGAACGCGCTGACGCTGTCGATCGACCACGGCGAGCTGCGCTGCGTGATCGGCCCCAACGGCGCTGGCAAGACCACG
ATGATGGATGTGATCACCGGCAAGACCGGTCCGCGCAACGCGGACGTCGCCGGCCGCGTCTACCTGGGCCAGGCCATCGA
CCTGCTGCGCCTGACCGAGCCGCGCATCGCGCAGATCGGCATCGGCCGCAAGTTCCAGAAGCCGACCGTGTTCGAGCAGC
ACGGCGTGTGGGAAAACCTCGAGCTGGCGATGCAGGCCGACAAGCGCTGGTGGGTGTCGCTGCGCGCGCGGCTGCTCGAC
GACGGCCGCCGCCGCATCGAAGAGACGCTCGGTCGCATCGGGCTGGAGGCCGAGGCGTACCGGAGCGCCGGCCTGCTGTC
GCACGGCCAGAAGCAGCGGCTGGAGATCGGCATGCTGCTGATGCAGCGGCCGCAGCTGCTGCTGCTCGACGAGCCCGTGG
CCGGCATGACCGATGAAGAGACGATGCAGCTGGCCGACCTGCTCAACGGCCTGCGCGGCGCGTGCTCGATCATGGTGGTG
GAGCACGACATGGAGTTCGTCGCCGCGCTGGCGGGCGAGACGGGCCGCGTGACCGTGCTGGCCGAAGGCAGCGTGCTGGC
CGAGGGCACGCTCGACGCGGTCAAGCGCGACGCGCGCGTGATCGAATCCTACCTGGGGCGCTAA

Upstream 100 bases:

>100_bases
TGCGTGGCCGCTCCGGGCGGCGCGTGCGGCCGGCGGCCGATGGCCGTGCGGATGCCGGCGCCGGCGAGCCCGTTGTCACC
CCGCCCGCCGGAGACCGCGC

Downstream 100 bases:

>100_bases
CGGCGAATCCGACATGCTGCAGATCCAGCAACTGAACCAGTACTACGGCGGCAGCCACATCCTGCGCAACGTCAGCTTCG
AGGTGCCGGCCGGCAAGCTG

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRPFTARPDRAQVGNFSGTTTGMGHVLEPDAIDVSHGPILYLEDVTVRFGGFRALNALTLSIDHGELRCVIGPNGAGKTT
MMDVITGKTGPRNADVAGRVYLGQAIDLLRLTEPRIAQIGIGRKFQKPTVFEQHGVWENLELAMQADKRWWVSLRARLLD
DGRRRIEETLGRIGLEAEAYRSAGLLSHGQKQRLEIGMLLMQRPQLLLLDEPVAGMTDEETMQLADLLNGLRGACSIMVV
EHDMEFVAALAGETGRVTVLAEGSVLAEGTLDAVKRDARVIESYLGR

Sequences:

>Translated_287_residues
MRPFTARPDRAQVGNFSGTTTGMGHVLEPDAIDVSHGPILYLEDVTVRFGGFRALNALTLSIDHGELRCVIGPNGAGKTT
MMDVITGKTGPRNADVAGRVYLGQAIDLLRLTEPRIAQIGIGRKFQKPTVFEQHGVWENLELAMQADKRWWVSLRARLLD
DGRRRIEETLGRIGLEAEAYRSAGLLSHGQKQRLEIGMLLMQRPQLLLLDEPVAGMTDEETMQLADLLNGLRGACSIMVV
EHDMEFVAALAGETGRVTVLAEGSVLAEGTLDAVKRDARVIESYLGR
>Mature_287_residues
MRPFTARPDRAQVGNFSGTTTGMGHVLEPDAIDVSHGPILYLEDVTVRFGGFRALNALTLSIDHGELRCVIGPNGAGKTT
MMDVITGKTGPRNADVAGRVYLGQAIDLLRLTEPRIAQIGIGRKFQKPTVFEQHGVWENLELAMQADKRWWVSLRARLLD
DGRRRIEETLGRIGLEAEAYRSAGLLSHGQKQRLEIGMLLMQRPQLLLLDEPVAGMTDEETMQLADLLNGLRGACSIMVV
EHDMEFVAALAGETGRVTVLAEGSVLAEGTLDAVKRDARVIESYLGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=250, Percent_Identity=28, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI150417984, Length=248, Percent_Identity=29.8387096774194, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27881501, Length=245, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI30795238, Length=245, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI46592956, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592964, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592978, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592971, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592898, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592915, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27436953, Length=231, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116734710, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI45446740, Length=231, Percent_Identity=25.974025974026, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI47078218, Length=231, Percent_Identity=25.974025974026, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=259, Percent_Identity=34.7490347490347, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790525, Length=243, Percent_Identity=28.8065843621399, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790544, Length=243, Percent_Identity=25.5144032921811, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87081791, Length=236, Percent_Identity=27.5423728813559, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082268, Length=257, Percent_Identity=30.7392996108949, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790739, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790190, Length=255, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789593, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081709, Length=249, Percent_Identity=26.1044176706827, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786872, Length=244, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787089, Length=254, Percent_Identity=27.1653543307087, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787792, Length=244, Percent_Identity=26.2295081967213, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787370, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786398, Length=227, Percent_Identity=28.1938325991189, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788225, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788053, Length=217, Percent_Identity=29.4930875576037, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789991, Length=240, Percent_Identity=27.5, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789672, Length=240, Percent_Identity=26.25, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787029, Length=237, Percent_Identity=23.2067510548523, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788897, Length=243, Percent_Identity=26.7489711934156, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI48995001, Length=233, Percent_Identity=28.755364806867, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788472, Length=250, Percent_Identity=24.8, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI48994943, Length=245, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790162, Length=218, Percent_Identity=26.1467889908257, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=240, Percent_Identity=27.5, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788761, Length=249, Percent_Identity=26.5060240963855, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789873, Length=204, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786563, Length=222, Percent_Identity=27.9279279279279, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081834, Length=223, Percent_Identity=26.457399103139, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787164, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788210, Length=252, Percent_Identity=25.3968253968254, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790467, Length=230, Percent_Identity=25.6521739130435, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787972, Length=245, Percent_Identity=28.1632653061224, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787105, Length=240, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789586, Length=243, Percent_Identity=24.2798353909465, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082267, Length=252, Percent_Identity=25.7936507936508, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983516, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565586, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574150, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007328, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581191, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581189, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581193, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581195, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24666092, Length=252, Percent_Identity=22.6190476190476, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24643674, Length=231, Percent_Identity=24.2424242424242, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85724864, Length=208, Percent_Identity=26.4423076923077, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24580555, Length=208, Percent_Identity=26.4423076923077, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI85816269, Length=208, Percent_Identity=26.4423076923077, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24580553, Length=208, Percent_Identity=26.4423076923077, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31441; Mature: 31441

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MRPFTARPDRAQVGNFSGTTTGMGHVLEPDAIDVSHGPILYLEDVTVRFGGFRALNALTL
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEECCCCCEEEEEEEEEEECCEEEEEEEEE
SIDHGELRCVIGPNGAGKTTMMDVITGKTGPRNADVAGRVYLGQAIDLLRLTEPRIAQIG
EEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEC
IGRKFQKPTVFEQHGVWENLELAMQADKRWWVSLRARLLDDGRRRIEETLGRIGLEAEAY
CCCCCCCCCCCHHCCCCCCHHHEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RSAGLLSHGQKQRLEIGMLLMQRPQLLLLDEPVAGMTDEETMQLADLLNGLRGACSIMVV
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
EHDMEFVAALAGETGRVTVLAEGSVLAEGTLDAVKRDARVIESYLGR
ECCHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPFTARPDRAQVGNFSGTTTGMGHVLEPDAIDVSHGPILYLEDVTVRFGGFRALNALTL
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEECCCCCEEEEEEEEEEECCEEEEEEEEE
SIDHGELRCVIGPNGAGKTTMMDVITGKTGPRNADVAGRVYLGQAIDLLRLTEPRIAQIG
EEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEC
IGRKFQKPTVFEQHGVWENLELAMQADKRWWVSLRARLLDDGRRRIEETLGRIGLEAEAY
CCCCCCCCCCCHHCCCCCCHHHEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RSAGLLSHGQKQRLEIGMLLMQRPQLLLLDEPVAGMTDEETMQLADLLNGLRGACSIMVV
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
EHDMEFVAALAGETGRVTVLAEGSVLAEGTLDAVKRDARVIESYLGR
ECCHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]