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The map label for this gene is murI

Identifier: 17546675

GI number: 17546675

Start: 2134800

End: 2135633

Strand: Direct

Name: murI

Synonym: RSc1956

Alternate gene names: 17546675

Gene position: 2134800-2135633 (Clockwise)

Preceding gene: 17546674

Following gene: 17546677

Centisome position: 57.44

GC content: 71.7

Gene sequence:

>834_bases
ATGACGACACGCGCGCAGGCACCGGTCGGGGTCTTCGACTCCGGCCTCGGAGGGCTCTCCGTCCTGCGCGCGATCCGTGC
CGAACTGCCGGCCGAATCCCTCCTCTACCTGGCGGATTCCCGCCATGCCCCGTACGGGGAAAAACCTCCTGAATTCATTG
CCGGGCGTACGCTGCGCGTGTGCGAGTGGCTGGTCGGCCAGGGCTGCAAGGCACTGGTGATCGCCTGCAACACGGCCACG
GCGCAGGCCGTGCACCTGCTGCGCGAGCAGCTCGCCGTGCCGGTGATCGGCGTCGAGCCGGGCCTGAAGCCGGCGGTGGC
CACATCGCGCAGCCGCGTGGTCGGCGTGCTGGCGACGGAAAGCACGCTGCGCAGCGACAAGTTCGCGCGCCTGCTGGGCA
ATGTCTCGGGGGATTGCCGCGTGCTGTGCCAGCCCGGCTACGGCCTCGTTCCGCTGATCGAGCGCGGCGACACGCACTCG
CCCGCCGTGCTGGAGCTGCTGCGGGCCTACCTGCTGCCGATGCTCGAAGCCGGCGCCGACACGCTGGTGCTCGGCTGCAC
GCACTACCCGTTCCTGCAGGACGCCATCCGCGAGATCGCCGGCGACCGCCTCACGCTGATCGACACCGGACACGCCGTGG
CGCGCCATCTCGGCCGCACGCTGGCCGCCGCGCACTTGCAGGCCACGGGGGCCGCCGCGTCCCCGCGCTTCCTGAGCACC
GCGGACGTGCTGCCGCTGCAGGCCATGGTGGCCGCGCTGCTGGGCGAGGCGCCAATGGCACAGCGGATCGACATCGGCGA
CACCGCCGTCCCGCCGCTCGCTTCGCACCAATAA

Upstream 100 bases:

>100_bases
GCGCGGGCACCTCGGTGCACAAGACCGGCCCGGCGGAATGGCAGGCCAAGATCGGCAAGATCCCGGTCGCCGCGGCGTGA
TCGGCTGACGCCATCCCGCC

Downstream 100 bases:

>100_bases
GCAATCCGCAATAAAAACGGGCCCGCAGAAACCTGCGGGCCCGTCATGTCATGGCCTCGGTGGGAGCGCGGCTCCCTGGA
CCAGCGGACTGCGCCGCCGG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKPPEFIAGRTLRVCEWLVGQGCKALVIACNTAT
AQAVHLLREQLAVPVIGVEPGLKPAVATSRSRVVGVLATESTLRSDKFARLLGNVSGDCRVLCQPGYGLVPLIERGDTHS
PAVLELLRAYLLPMLEAGADTLVLGCTHYPFLQDAIREIAGDRLTLIDTGHAVARHLGRTLAAAHLQATGAAASPRFLST
ADVLPLQAMVAALLGEAPMAQRIDIGDTAVPPLASHQ

Sequences:

>Translated_277_residues
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKPPEFIAGRTLRVCEWLVGQGCKALVIACNTAT
AQAVHLLREQLAVPVIGVEPGLKPAVATSRSRVVGVLATESTLRSDKFARLLGNVSGDCRVLCQPGYGLVPLIERGDTHS
PAVLELLRAYLLPMLEAGADTLVLGCTHYPFLQDAIREIAGDRLTLIDTGHAVARHLGRTLAAAHLQATGAAASPRFLST
ADVLPLQAMVAALLGEAPMAQRIDIGDTAVPPLASHQ
>Mature_276_residues
TTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKPPEFIAGRTLRVCEWLVGQGCKALVIACNTATA
QAVHLLREQLAVPVIGVEPGLKPAVATSRSRVVGVLATESTLRSDKFARLLGNVSGDCRVLCQPGYGLVPLIERGDTHSP
AVLELLRAYLLPMLEAGADTLVLGCTHYPFLQDAIREIAGDRLTLIDTGHAVARHLGRTLAAAHLQATGAAASPRFLSTA
DVLPLQAMVAALLGEAPMAQRIDIGDTAVPPLASHQ

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=232, Percent_Identity=35.3448275862069, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_RALSO (Q8XY07)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520077.1
- ProteinModelPortal:   Q8XY07
- SMR:   Q8XY07
- GeneID:   1220796
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1956
- NMPDR:   fig|267608.1.peg.1956
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- BioCyc:   RSOL267608:RSC1956-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29136; Mature: 29005

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKPPEFIAGRTLRV
CCCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHH
CEWLVGQGCKALVIACNTATAQAVHLLREQLAVPVIGVEPGLKPAVATSRSRVVGVLATE
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCCEEEEEECH
STLRSDKFARLLGNVSGDCRVLCQPGYGLVPLIERGDTHSPAVLELLRAYLLPMLEAGAD
HHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TLVLGCTHYPFLQDAIREIAGDRLTLIDTGHAVARHLGRTLAAAHLQATGAAASPRFLST
EEEEECCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ADVLPLQAMVAALLGEAPMAQRIDIGDTAVPPLASHQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTRAQAPVGVFDSGLGGLSVLRAIRAELPAESLLYLADSRHAPYGEKPPEFIAGRTLRV
CCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHH
CEWLVGQGCKALVIACNTATAQAVHLLREQLAVPVIGVEPGLKPAVATSRSRVVGVLATE
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCCEEEEEECH
STLRSDKFARLLGNVSGDCRVLCQPGYGLVPLIERGDTHSPAVLELLRAYLLPMLEAGAD
HHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TLVLGCTHYPFLQDAIREIAGDRLTLIDTGHAVARHLGRTLAAAHLQATGAAASPRFLST
EEEEECCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ADVLPLQAMVAALLGEAPMAQRIDIGDTAVPPLASHQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852