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The map label for this gene is fumA [H]

Identifier: 17546674

GI number: 17546674

Start: 2133256

End: 2134779

Strand: Direct

Name: fumA [H]

Synonym: RSc1955

Alternate gene names: 17546674

Gene position: 2133256-2134779 (Clockwise)

Preceding gene: 17546673

Following gene: 17546675

Centisome position: 57.4

GC content: 66.4

Gene sequence:

>1524_bases
ATGACCGTCATTCGTCAAGAAGACCTCATCCAGAGCGTCGCCGACTCCCTGCAGTACATCAGCTATTACCACCCGATGGA
CTACATCACTGCTCTGGGCCAGGCCTATGAGCAGGAGCAGAGCCCGGCCGCCAAGGATGCCATCGCGCAGATCCTGACCA
ACAGCCGCATGTGCGCCGAAGGCAAGCGCCCGATCTGCCAGGACACCGGCATCGTCACCGTGTTCCTGAAAGTCGGCATG
AACGTGCGCTGGGACGGCGCCACGATGAGCCTGGAAGACATGGTCAACGAAGGCGTGCGCCGCGCCTATAACGACCAGGA
CAACAAGCTGCGCGCCAGCGTCCTGGCCGACCCGGCCGGCAAGCGCACCAACACGAAGGACAACACCCCGGCGGTGATCA
ACATGTCCATCGTGCCGGGCGACACAGTGGACGTGATCGTCGCGGCCAAGGGCGGCGGCTCCGAGGCCAAGTCCAAGTTC
GTGATGCTCAACCCGTCCGACTCGATCGTCGACTGGGTGCTCAAGACGGTGCCGACCATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTGGGCATCGGCATCGGCGGCACCGCCGAGAAGGCGATGCTGCTGGCCAAGGAAGCCCTGATGGAGCCGATCG
ACATCCAGGACCTGATCGCGCGCGGCCCGTCGAACCGCGCCGAAGAGCTGCGCATCGAGCTCTACGAAAAGGTCAACGCA
CTGGGTATCGGGGCCCAGGGCCTGGGCGGCCTCGCCACCGTGCTCGACGTGAAGATCAAGGACTACCCGACCCACGCCGC
CAACCTGCCGGTGGCCATGATCCCGAACTGCGCGGCCACCCGCCACGCGCACTTCACGCTGGACGGCAGCGGCGTCGCCA
AGCTCGAAGCGCCGGACCTGTCGCAATGGCCGAAGGTCGAGTGGGCGCCGAACACCGAAACCTCCAAGCGCGTTGATCTG
AACGCGCTCACGCCGGAAGAAGTCGCCTCGTGGAAGCCGGGCCAAACCCTGCTGCTCAACGGCAAGATGCTCACGGGCCG
CGACGCCGCGCACAAGCGCATCGCCGACATGCTGGCCCGCGGCGAACAGTTGCCGATCGACTTCACCAACCGCGTGATCT
ACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAGGTGGTCGGTCCGGCCGGCCCCACCACGGCCACGCGCATGGACAAG
TTCACCGAGACGATGCTCGCCAAGACCGGCCTGATCGCCATGGTCGGCAAGGCCGAGCGCGGTCCGGTCGCCATCGAGGC
CATCCAGAAGCACAAGTCGGCCTACCTGATGGCCGTGGGCGGCGCGGCCTACCTTGTGTCCAAGGCCATCCGCGGCGCGA
AGGTGCTGGCATTCGAAGACCTGGGCATGGAAGCCATCTACGAATTCGACGTGAAGGACATGCCCGTCACCGTGGCGGTC
GACAGCGCGGGCACCTCGGTGCACAAGACCGGCCCGGCGGAATGGCAGGCCAAGATCGGCAAGATCCCGGTCGCCGCGGC
GTGA

Upstream 100 bases:

>100_bases
GGTCTGAGCCGGCGGCGCCCTGCCGCCGGTACGCCGCGCAGGCCACTGCCGCTGCGGTTGCCGCACCCGGTTTCCCATTC
CTACATCGTTGGTCCCCACC

Downstream 100 bases:

>100_bases
TCGGCTGACGCCATCCCGCCATGACGACACGCGCGCAGGCACCGGTCGGGGTCTTCGACTCCGGCCTCGGAGGGCTCTCC
GTCCTGCGCGCGATCCGTGC

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIRQEDLIQSVADSLQYISYYHPMDYITALGQAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGM
NVRWDGATMSLEDMVNEGVRRAYNDQDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
LGIGAQGLGGLATVLDVKIKDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDL
NALTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLARGEQLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAKTGLIAMVGKAERGPVAIEAIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVKDMPVTVAV
DSAGTSVHKTGPAEWQAKIGKIPVAAA

Sequences:

>Translated_507_residues
MTVIRQEDLIQSVADSLQYISYYHPMDYITALGQAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGM
NVRWDGATMSLEDMVNEGVRRAYNDQDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
LGIGAQGLGGLATVLDVKIKDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDL
NALTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLARGEQLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDK
FTETMLAKTGLIAMVGKAERGPVAIEAIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVKDMPVTVAV
DSAGTSVHKTGPAEWQAKIGKIPVAAA
>Mature_506_residues
TVIRQEDLIQSVADSLQYISYYHPMDYITALGQAYEQEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFLKVGMN
VRWDGATMSLEDMVNEGVRRAYNDQDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNAL
GIGAQGLGGLATVLDVKIKDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKVEWAPNTETSKRVDLN
ALTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLARGEQLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKF
TETMLAKTGLIAMVGKAERGPVAIEAIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVKDMPVTVAVD
SAGTSVHKTGPAEWQAKIGKIPVAAA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=469, Percent_Identity=30.9168443496802, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1787897, Length=466, Percent_Identity=31.1158798283262, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1789442, Length=290, Percent_Identity=30, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.9329608938547, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54337; Mature: 54206

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRQEDLIQSVADSLQYISYYHPMDYITALGQAYEQEQSPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDQDNKLRASVLADPAG
CCCCCCCCCCCEEEEEHHCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIKDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCC
SQWPKVEWAPNTETSKRVDLNALTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAR
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEQLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAKTGLIAMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GPVAIEAIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVKDMPVTVAV
CCHHHHHHHHCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEEE
DSAGTSVHKTGPAEWQAKIGKIPVAAA
CCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIRQEDLIQSVADSLQYISYYHPMDYITALGQAYEQEQSPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDQDNKLRASVLADPAG
CCCCCCCCCCCEEEEEHHCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLIARGPSNRAEELRIELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIKDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCC
SQWPKVEWAPNTETSKRVDLNALTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAR
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEQLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAKTGLIAMVGKAER
CCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GPVAIEAIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVKDMPVTVAV
CCHHHHHHHHCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEEE
DSAGTSVHKTGPAEWQAKIGKIPVAAA
CCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]