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The map label for this gene is emrB [H]

Identifier: 17546571

GI number: 17546571

Start: 2034095

End: 2035633

Strand: Reverse

Name: emrB [H]

Synonym: RSc1852

Alternate gene names: 17546571

Gene position: 2035633-2034095 (Counterclockwise)

Preceding gene: 17546572

Following gene: 17546569

Centisome position: 54.77

GC content: 66.15

Gene sequence:

>1539_bases
ATGACTACCCCGTCTTCAGACAGCCAGGCGCCCCTGACCGGGGGAATGCTGTGGTTGGCGGCCATTGTTCTCGCGGCGGC
CAACTTCGTCGCCGTGCTGGACATGACTATCGCCAACGTCTCGGTTCCCAACATCTCCGGCAATCTCGGGGTCTCGACGA
GTCAGGGCACCTGGGTCATCACGTCCTACGCCGTGGCCGAAGCCATCCTCGTGCCGCTCACCGGTTGGCTGGCATCGCGC
TTCGGCACGGTGAGGGTGTTTGCCGTAGCAATGGGATGTTTCGGCCTGTTCTCGGCCCTGTGCGGCCTGGCCCTTTCGTT
GGGGATGCTGGTGGCAGCGCGCATCTGCCAGGGGCTGGCCGGCGGGCCGCTGATGCCGCTGTCGCAAACGCTGCTGCTGC
GCATTTTTCCGAAGGAGAAGGCGGGGGCTGCCATCGGCCTGTGGTCGATGACGACCATCGTGGCCCCGGTGCTTGGCCCC
ATTCTGGGCGGCTACCTATGCGATGAATACAGCTGGCCCTGGATCTTCTACATCAACCTGCCCATCGCTGTGGGCTGCGC
CTGGGCGGCGTGGAAGATGCTGGCGCGCTACGAATCGCCCATTGCCCGCAACCCCATCGACAAGGTGGGGCTGGCCTTGC
TGGTGCTGTGGGTGGGCTCGTTGCAAATCATGCTCGACGAGGGCAAGCAACTGGACTGGTTCGCTTCCTCGACCATCGCC
GGCTTGGCGGTCGTCGCCGCCATCGGCTTCGCGGCCTTCCTGATCTGGGAGCTGCACGCCGAGCATCCGGCGGTGAACCT
GCGGGTGTTTCGGCACCGTGGGTTTTCCGCCAGCGTGCTGACTATCAGTCTGGCCTTTGGCGCGTTTTTCGGCGCGAATG
TGCTCACGCCGCAATGGTTGCAGAGCTTCATGGGCTACACCGCGCTGGAGGCGGGCAAGACTACCGCCTGGAGCGGCTTC
TTCGCGCTGTTCGCGGCGCCCATCGCGGCAGGGCTCGGGGGCAAGGTCGACGCGCGCAAGCTGGTCTTCGGCGGGGTCAT
CTGGCTGGGCATCATCACCCTGTGGCGCACCGTCGCCACCACCGACATGACCTACTGGCAGGTGGCCGTGCCGCTGATGG
CGATGGGGCTGGGCATTCCCTTCTTCTTCGTGCCCACCACGGGCCTGGCGATGGCCAGCGTGGACGAGGAAGAAATGGCT
TCCGCCGCCGGGCTGATGAACTTCCTGCGCACCTTGTCGGGGGCCTTCGCGACCTCGCTCATCACCACGCTGTGGGATGA
CCGCGCCACGCTCCATCACGCGGAACTGGTCGGCGTGTCCGACGGCGACGGCAGCGTGCGCACGATGCTGGAGGGGGCGG
GCTCCACCTTGGACGCCATCAACCAGTCCATCGACCAGCTCCTCACCAGCCAAAGCGTGATGCTGGCCACCAACGACATC
ATGGCCTGGGTGGGCGTGGCCTTCGTGCTGGCGGCGAGCGCCATCTGGCTTGCGCCCAAGCCACGCAGGACGGTGGCGCC
GGGCACCGGGGGGCATTGA

Upstream 100 bases:

>100_bases
AAAGCACCCGCTGAAAGTGGGGCTGTCGATGGTGGTCACGATCGACACCAGCGCCTCCGGTAGCCAGGCCACCCAGGCCC
GTCGGTAAAGGAGGGGCCTC

Downstream 100 bases:

>100_bases
TATGGTTTGGGGCCAGTCCTTGCGCTTCGCGTTCCGACCAGGGCTGCCGCGAGCGGGGCAGGGCCCATGCGCGGCGGACC
TTCAATCCGGACTTCGACTG

Product: multidrug resistance transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTTPSSDSQAPLTGGMLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVITSYAVAEAILVPLTGWLASR
FGTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLAGGPLMPLSQTLLLRIFPKEKAGAAIGLWSMTTIVAPVLGP
ILGGYLCDEYSWPWIFYINLPIAVGCAWAAWKMLARYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIA
GLAVVAAIGFAAFLIWELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWLQSFMGYTALEAGKTTAWSGF
FALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVATTDMTYWQVAVPLMAMGLGIPFFFVPTTGLAMASVDEEEMA
SAAGLMNFLRTLSGAFATSLITTLWDDRATLHHAELVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDI
MAWVGVAFVLAASAIWLAPKPRRTVAPGTGGH

Sequences:

>Translated_512_residues
MTTPSSDSQAPLTGGMLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVITSYAVAEAILVPLTGWLASR
FGTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLAGGPLMPLSQTLLLRIFPKEKAGAAIGLWSMTTIVAPVLGP
ILGGYLCDEYSWPWIFYINLPIAVGCAWAAWKMLARYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIA
GLAVVAAIGFAAFLIWELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWLQSFMGYTALEAGKTTAWSGF
FALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVATTDMTYWQVAVPLMAMGLGIPFFFVPTTGLAMASVDEEEMA
SAAGLMNFLRTLSGAFATSLITTLWDDRATLHHAELVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDI
MAWVGVAFVLAASAIWLAPKPRRTVAPGTGGH
>Mature_511_residues
TTPSSDSQAPLTGGMLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVITSYAVAEAILVPLTGWLASRF
GTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLAGGPLMPLSQTLLLRIFPKEKAGAAIGLWSMTTIVAPVLGPI
LGGYLCDEYSWPWIFYINLPIAVGCAWAAWKMLARYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIAG
LAVVAAIGFAAFLIWELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWLQSFMGYTALEAGKTTAWSGFF
ALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVATTDMTYWQVAVPLMAMGLGIPFFFVPTTGLAMASVDEEEMAS
AAGLMNFLRTLSGAFATSLITTLWDDRATLHHAELVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDIM
AWVGVAFVLAASAIWLAPKPRRTVAPGTGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=43.4, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=41.7821782178218, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=29.7101449275362, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=28.4009546539379, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=28.2828282828283, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=178, Percent_Identity=30.3370786516854, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=207, Percent_Identity=28.9855072463768, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=136, Percent_Identity=30.1470588235294, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54043; Mature: 53912

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSSDSQAPLTGGMLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCEEE
TSYAVAEAILVPLTGWLASRFGTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLA
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGPLMPLSQTLLLRIFPKEKAGAAIGLWSMTTIVAPVLGPILGGYLCDEYSWPWIFYINL
CCCCCHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PIAVGCAWAAWKMLARYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHH
GLAVVAAIGFAAFLIWELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHH
QSFMGYTALEAGKTTAWSGFFALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVAT
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TDMTYWQVAVPLMAMGLGIPFFFVPTTGLAMASVDEEEMASAAGLMNFLRTLSGAFATSL
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITTLWDDRATLHHAELVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDI
HHHHHCCCHHEEHHEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEHHHH
MAWVGVAFVLAASAIWLAPKPRRTVAPGTGGH
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPSSDSQAPLTGGMLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCEEE
TSYAVAEAILVPLTGWLASRFGTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLA
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGPLMPLSQTLLLRIFPKEKAGAAIGLWSMTTIVAPVLGPILGGYLCDEYSWPWIFYINL
CCCCCHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PIAVGCAWAAWKMLARYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHH
GLAVVAAIGFAAFLIWELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHH
QSFMGYTALEAGKTTAWSGFFALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVAT
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TDMTYWQVAVPLMAMGLGIPFFFVPTTGLAMASVDEEEMASAAGLMNFLRTLSGAFATSL
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITTLWDDRATLHHAELVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDI
HHHHHCCCHHEEHHEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEHHHH
MAWVGVAFVLAASAIWLAPKPRRTVAPGTGGH
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]