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The map label for this gene is thcR [H]

Identifier: 17546551

GI number: 17546551

Start: 2003334

End: 2004362

Strand: Direct

Name: thcR [H]

Synonym: RSc1832

Alternate gene names: 17546551

Gene position: 2003334-2004362 (Clockwise)

Preceding gene: 17546539

Following gene: 17546559

Centisome position: 53.91

GC content: 67.35

Gene sequence:

>1029_bases
ATGTTGAACACAACGCCCCCACTGACCGTAGCGCAGCGTCCGGCAGGGTTGTCGGCCGCCCTCGATCCGTTTCCGTGCAC
ACCGGAACGGTTGCTGTTCGCCTCCGCCGACCTCGATGAAATCCGCTCCATGGTCGGACGGGTCATGAAACCGCACCGCC
TGGCGCTGGTGGGCGGCGCGGATCGCCTCGATGCGCGCATGCACTACACGCCACTCGGCGACATCTCGCTCAGCCGCTTG
CGGTACGGCGCGGCCGTCGAGATCGAGCCCGGCCCCCTCGACAGCTTCTTCCTGGTGCAGATGCCCATTTCGGGGAGCGC
GGAAGTCGAGAGCGGTGCACAGCACATCGACTCCGGTCCGGATGTCGCCTCGGTCCTCAGCCCCGAAGAGGGCACCAAGA
TGCGCTGGTCCGCGGGCAACGAGCAGCTGATGCTGCGCCTGTCGCGCTCCCTCGTCGAGCGGACGCTGGTCGGCCATTTC
GGCCATCCGCTGGAGCAGCCCCTGCGTTTCGAGCTTGGCTTCGAATGGCGGGGCTGCGCGCAGTGGCGGTGCCTGCTGAG
CTATCTGATCGATTGCTCGACGCAGGGCCTCGACCTGGCGCAGCACAAGCTCGTCATCGCGCAGATCGAGCAGTTGGCCG
CTTCGGTGCTGCTGGCCTCGCACCAGCACAACTACAGCGAGGCCGCGCCGTCGCGGCGGGGGACGATCCTGCCGCGCCAC
GTCAGGCGCGCGCAGGACTATCTGCAGGCCCATGCCCACGAGCCCGTCTGCGCAGACCAGCTGGCCCAGGTCGTGGGCGT
CAGCGTGCGCAGCCTGTACACGGGCTTCAAGGAGTTTCTCGGCGTCAGCCCGATGCACTATCTGCGCGACCTGCGCATGG
AGCGCACACGGACCGAGCTGATGAGCGGCGAAGCCACCAATGTGGCGGGCGTTGCCCTGCGCTGGGGATTCGCCCACATG
GGCCGTTTCAGCAACGACTACAAGCAGCGCTACGGAGAAACCCCCAGCCAGACGCTGCGCCGCAGATAG

Upstream 100 bases:

>100_bases
GGTCTTGCAAGTCGTGGCGGCGTCCACGGGCCGGGTCGGCCCGAAGGTGAAGCTGCCATGATCGACGCGACACTCGGCCC
CAGCCCAGGAGGCAAAGCCC

Downstream 100 bases:

>100_bases
CGCGGCGAGATCGGCCGGCGCTGCCTGCCGCACGCACACGCGGGCCGCGCATGGCGGGCGGAAAGCCGCCGGGGCAATGC
GCCGGCGGCTGCGTGCCGAC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLNTTPPLTVAQRPAGLSAALDPFPCTPERLLFASADLDEIRSMVGRVMKPHRLALVGGADRLDARMHYTPLGDISLSRL
RYGAAVEIEPGPLDSFFLVQMPISGSAEVESGAQHIDSGPDVASVLSPEEGTKMRWSAGNEQLMLRLSRSLVERTLVGHF
GHPLEQPLRFELGFEWRGCAQWRCLLSYLIDCSTQGLDLAQHKLVIAQIEQLAASVLLASHQHNYSEAAPSRRGTILPRH
VRRAQDYLQAHAHEPVCADQLAQVVGVSVRSLYTGFKEFLGVSPMHYLRDLRMERTRTELMSGEATNVAGVALRWGFAHM
GRFSNDYKQRYGETPSQTLRRR

Sequences:

>Translated_342_residues
MLNTTPPLTVAQRPAGLSAALDPFPCTPERLLFASADLDEIRSMVGRVMKPHRLALVGGADRLDARMHYTPLGDISLSRL
RYGAAVEIEPGPLDSFFLVQMPISGSAEVESGAQHIDSGPDVASVLSPEEGTKMRWSAGNEQLMLRLSRSLVERTLVGHF
GHPLEQPLRFELGFEWRGCAQWRCLLSYLIDCSTQGLDLAQHKLVIAQIEQLAASVLLASHQHNYSEAAPSRRGTILPRH
VRRAQDYLQAHAHEPVCADQLAQVVGVSVRSLYTGFKEFLGVSPMHYLRDLRMERTRTELMSGEATNVAGVALRWGFAHM
GRFSNDYKQRYGETPSQTLRRR
>Mature_342_residues
MLNTTPPLTVAQRPAGLSAALDPFPCTPERLLFASADLDEIRSMVGRVMKPHRLALVGGADRLDARMHYTPLGDISLSRL
RYGAAVEIEPGPLDSFFLVQMPISGSAEVESGAQHIDSGPDVASVLSPEEGTKMRWSAGNEQLMLRLSRSLVERTLVGHF
GHPLEQPLRFELGFEWRGCAQWRCLLSYLIDCSTQGLDLAQHKLVIAQIEQLAASVLLASHQHNYSEAAPSRRGTILPRH
VRRAQDYLQAHAHEPVCADQLAQVVGVSVRSLYTGFKEFLGVSPMHYLRDLRMERTRTELMSGEATNVAGVALRWGFAHM
GRFSNDYKQRYGETPSQTLRRR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=106, Percent_Identity=36.7924528301887, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787649, Length=255, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38051; Mature: 38051

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTTPPLTVAQRPAGLSAALDPFPCTPERLLFASADLDEIRSMVGRVMKPHRLALVGGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCEEEEECCC
DRLDARMHYTPLGDISLSRLRYGAAVEIEPGPLDSFFLVQMPISGSAEVESGAQHIDSGP
HHHCCEEEECCCCCCHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCCC
DVASVLSPEEGTKMRWSAGNEQLMLRLSRSLVERTLVGHFGHPLEQPLRFELGFEWRGCA
CHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
QWRCLLSYLIDCSTQGLDLAQHKLVIAQIEQLAASVLLASHQHNYSEAAPSRRGTILPRH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHH
VRRAQDYLQAHAHEPVCADQLAQVVGVSVRSLYTGFKEFLGVSPMHYLRDLRMERTRTEL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MSGEATNVAGVALRWGFAHMGRFSNDYKQRYGETPSQTLRRR
HCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MLNTTPPLTVAQRPAGLSAALDPFPCTPERLLFASADLDEIRSMVGRVMKPHRLALVGGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCEEEEECCC
DRLDARMHYTPLGDISLSRLRYGAAVEIEPGPLDSFFLVQMPISGSAEVESGAQHIDSGP
HHHCCEEEECCCCCCHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCCC
DVASVLSPEEGTKMRWSAGNEQLMLRLSRSLVERTLVGHFGHPLEQPLRFELGFEWRGCA
CHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
QWRCLLSYLIDCSTQGLDLAQHKLVIAQIEQLAASVLLASHQHNYSEAAPSRRGTILPRH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHH
VRRAQDYLQAHAHEPVCADQLAQVVGVSVRSLYTGFKEFLGVSPMHYLRDLRMERTRTEL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MSGEATNVAGVALRWGFAHMGRFSNDYKQRYGETPSQTLRRR
HCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]