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The map label for this gene is xthA [H]

Identifier: 17546312

GI number: 17546312

Start: 1708234

End: 1709043

Strand: Reverse

Name: xthA [H]

Synonym: RSc1593

Alternate gene names: 17546312

Gene position: 1709043-1708234 (Counterclockwise)

Preceding gene: 17546314

Following gene: 17546311

Centisome position: 45.99

GC content: 67.53

Gene sequence:

>810_bases
ATGCGTGTCGCCACCTGGAACGTCAACTCCCTCAAAGTCCGCCTGCCGCACGTCCTGCAATGGCTGGGCGAGCGCGAAGC
CGACGCCACGCCCATCGACCTGCTCTGCCTGCAGGAACTGAAGCTGCCCGATGACCGCTACCCGCTGGCCGAGCTCGAGG
CCGCCGGCTATGCCTCGCTGTTCACCGGCCAGAAGACCTACAACGGCGTTGCCATCCTCGCGCGCAAGGCGGCCATGCCC
GAGGGCCGCGATGTGGTCAGGAACATCCCGGACTTTGCCGACGAGCAGCAGCGCGTCGTGGCTGCCACGTACGATGTGGC
CGGCGGCCCGGTGCGGGTGATCTCCGCCTACTTCCCCAACGGCCAGGCGCTCGATTCCGACAAGATGGTCTACAAGATGC
GCTGGCTGGCCGCCCTGCAGGACTGGCTGCAGGCCGAGATGGCCGCGCACCCGCGCCTGATGCTGCTGGGCGATTTCAAC
ATCGCCCCCGACGACCGCGACGTGCACGACCCGAAGAAGTGGGAAGGCATGAACCTCGTCTCGCCGGAGGAGCGTGCCGC
GTTCCGCGCGCTGGAAAGCGCCGGGCTGGTCGACGCCTTCCGCATGTTCGAGCAGGAAGACAAGCTGTTCTCGTGGTGGG
ACTACCGCCTGTTCGCGTTCAGGCGCAATGCCGGACTGCGCATCGACCACATCCTGCTGTCGCCGAACCTGGCCCGGCTG
TGCGAGTCGTGCCATATCGACCGCGTGCCGCGCGGCTGGGAACAGCCTTCGGACCATACGCCCGTGGTCGCCGCCCTGCG
TGACGCCTGA

Upstream 100 bases:

>100_bases
CGTCGGATGCCGGGCGGAGGCTTGGTGGCACAATAGCGCTCCATCCGGGCGGCGCTGCATCGGCACCGCCGTCATCTTTT
TTGCCTGAACCGATTTCCCC

Downstream 100 bases:

>100_bases
GCCCGCGCGATCCGCATGGTTTCGACCTTCAAGCGCCGCAACCTACCGCACTTGCTGCTGCGTGCCCGCGAGACGCTCAT
GGCGCGTTTCCGGCCCATCC

Product: exodeoxyribonuclease III protein

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRVATWNVNSLKVRLPHVLQWLGEREADATPIDLLCLQELKLPDDRYPLAELEAAGYASLFTGQKTYNGVAILARKAAMP
EGRDVVRNIPDFADEQQRVVAATYDVAGGPVRVISAYFPNGQALDSDKMVYKMRWLAALQDWLQAEMAAHPRLMLLGDFN
IAPDDRDVHDPKKWEGMNLVSPEERAAFRALESAGLVDAFRMFEQEDKLFSWWDYRLFAFRRNAGLRIDHILLSPNLARL
CESCHIDRVPRGWEQPSDHTPVVAALRDA

Sequences:

>Translated_269_residues
MRVATWNVNSLKVRLPHVLQWLGEREADATPIDLLCLQELKLPDDRYPLAELEAAGYASLFTGQKTYNGVAILARKAAMP
EGRDVVRNIPDFADEQQRVVAATYDVAGGPVRVISAYFPNGQALDSDKMVYKMRWLAALQDWLQAEMAAHPRLMLLGDFN
IAPDDRDVHDPKKWEGMNLVSPEERAAFRALESAGLVDAFRMFEQEDKLFSWWDYRLFAFRRNAGLRIDHILLSPNLARL
CESCHIDRVPRGWEQPSDHTPVVAALRDA
>Mature_269_residues
MRVATWNVNSLKVRLPHVLQWLGEREADATPIDLLCLQELKLPDDRYPLAELEAAGYASLFTGQKTYNGVAILARKAAMP
EGRDVVRNIPDFADEQQRVVAATYDVAGGPVRVISAYFPNGQALDSDKMVYKMRWLAALQDWLQAEMAAHPRLMLLGDFN
IAPDDRDVHDPKKWEGMNLVSPEERAAFRALESAGLVDAFRMFEQEDKLFSWWDYRLFAFRRNAGLRIDHILLSPNLARL
CESCHIDRVPRGWEQPSDHTPVVAALRDA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=276, Percent_Identity=30.0724637681159, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375503, Length=276, Percent_Identity=30.0724637681159, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375501, Length=276, Percent_Identity=30.0724637681159, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788046, Length=276, Percent_Identity=37.3188405797101, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30557; Mature: 30557

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVATWNVNSLKVRLPHVLQWLGEREADATPIDLLCLQELKLPDDRYPLAELEAAGYASL
CEEEEECCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCHHH
FTGQKTYNGVAILARKAAMPEGRDVVRNIPDFADEQQRVVAATYDVAGGPVRVISAYFPN
HCCCCCCCCCEEEEHHHCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCC
GQALDSDKMVYKMRWLAALQDWLQAEMAAHPRLMLLGDFNIAPDDRDVHDPKKWEGMNLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCC
SPEERAAFRALESAGLVDAFRMFEQEDKLFSWWDYRLFAFRRNAGLRIDHILLSPNLARL
CHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCEEEEEEECCCHHHH
CESCHIDRVPRGWEQPSDHTPVVAALRDA
HHHCCCCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MRVATWNVNSLKVRLPHVLQWLGEREADATPIDLLCLQELKLPDDRYPLAELEAAGYASL
CEEEEECCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCHHH
FTGQKTYNGVAILARKAAMPEGRDVVRNIPDFADEQQRVVAATYDVAGGPVRVISAYFPN
HCCCCCCCCCEEEEHHHCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCC
GQALDSDKMVYKMRWLAALQDWLQAEMAAHPRLMLLGDFNIAPDDRDVHDPKKWEGMNLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCC
SPEERAAFRALESAGLVDAFRMFEQEDKLFSWWDYRLFAFRRNAGLRIDHILLSPNLARL
CHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCEEEEEEECCCHHHH
CESCHIDRVPRGWEQPSDHTPVVAALRDA
HHHCCCCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]