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The map label for this gene is rluB [H]

Identifier: 17546005

GI number: 17546005

Start: 1367863

End: 1369659

Strand: Direct

Name: rluB [H]

Synonym: RSc1286

Alternate gene names: 17546005

Gene position: 1367863-1369659 (Clockwise)

Preceding gene: 17546004

Following gene: 17546006

Centisome position: 36.81

GC content: 70.34

Gene sequence:

>1797_bases
ATGTCGGCCGATGCCAATGCAGCGGCGCCTCGCCAGGATGGCGCCGGAGAGGGCGAGGAATCGTCCGCGCCGCGCCGCAA
GGGTCTGCGCCGCGGCCTGCGCAACCTAGTCGCCGCGCGGCGGCAGCAGCATGAGGGCCGCGCCGAAAGCGAGCCCGCGG
GCGAGGGTGCGGCGAGCGCCGGCGAAGGCGCGCAAGCGCCGCGCAAGGCTCGCGCACCGCGCAGCCGCAAGCCGAAAGAG
GCGAATGCCGCAGCGGGCGAGGTTGTGGCCATTCCGGAGGCGATGAGTTTGTCGGCGGAAGGGCGGCCGTCCGCGGCGGA
GGCCTCGGCCGAGCGTCGGGGAGCGCGTGGCCGCTTCGGCAAGGGCCGCCGCCAGGCTGAGGGCCAGCCGGCCGGCGATC
GCGCGGAAGGTCAGGGCGGCAATGCCGGCGGCGGCAACGGCGGCCGGGGTAAGAACGCCCGCCCGACCCGTGGCGCCAAG
GGGCAAGGCAAGGCGGCCGGCCAGAGCCGGGGCAAGGGCGGCAAGGGTGCCGAGGACGTCTTCCAGTACGTGATTTCCGG
CGCCTATGATGCTGACGCGGATGTGGGCGGCGCGCCGCAGTCGACCAAGGTGCGCGAACTCACTGCCGAGGACGACGCCC
CGAAGCTGCACAAGATTCTCGCCGATGCGGGCCTGGGCTCGCGTCGCGACATGGAAGACCTGATCCTGCAGGGCCGCGTC
TCCGTGAACGGCTTGCCGGCGCATATTGGCCAGCGCATCCTGCCGACCGATCAGGTGCGCGTCAACGGCAAGCTCATCCA
GCGCAAGCTGCCGAGCAAGCCGCCGCGCGTGCTGCTGTATCACAAGCCGGCCGGCGAGATCGTCAGCCAGTCTGATCCCG
AGGGCCGTCCGACCGTGTTCGATTCCCTGCCGCGCATGAAGACCGGCAAGTGGGTCGCCGTGGGCCGCCTGGACTTCAAC
ACCGAAGGCTTGCTGATCTTCACCACGTCGGGCGATATCGCCAATCGGTTCATGCATCCGCGCTACGGCGTCGAACGTGA
ATATGCGGTGCGCACGCTGGGCGAGCTGCCGGAGTCCGACCGCCAGAAACTGTTGCAGGGCATCCGCCTGGATGACGGCG
AAGCGAATTTCCTGCGCTGCGCGGATGGCGGTGGTGAGGGCGTCAATCACTGGTACCACGTCGCGCTCACCGAAGGTCGC
AACCGCGAGGTCCGCCGCATGTTCGAGGCCGTCAACCTGACGGTGTCGCGCCTGATCCGCACGCGCTATGGCAGTTTCGT
GCTGCCGCGCGGCCTCAAGCGCGGCCGCTGGCAGGAGGTGTCGGCCGAGGATGTCCGGGGGCTGATGGGGTTGCTGGGCT
TGAAAGTGCCTTCGGCAAGTGCCAGCGGCGGCCAGGGTAGTCAGGGTGGCCGACAGGGTAACCGTCGCCGCGAGGCGCAG
CCGGTGCTGATGGGGCCGATGTCGTCCGGCTTCACGGGCGAGGCGACGTTCCAGTCGCGCGGCCAGGGTATCGAAAACGG
CAACCGTGCGATGCCGGCTCCGCGTCGCCAGTCCAACCCGCGTCAGCCGGACCCGATGCAGACCTCGATGGGCTACATCA
ACGTTGGCGGACCGACCACGCTGACCGCGCGGACCCGCATGGGCGGTGGCCGTGGCCTGCCGGGTGCCGGCAGCAGCAAT
AGCGGTGGTAGCGGCAATGGCAACCGTGCGCCGGGCAACAAGCGTCCGGCCAAGGGCGGTGGCGGCTTGCCGAACGGCAA
CAAGGCGCGTGGCGGCGGCAAGCGCGGCAAGCGCTGA

Upstream 100 bases:

>100_bases
ATCCATAGGCCGTGTCTGCCGCGCGACCGGTGCATGGCCTGAACCCCTTTTTGATCTGAATGACGCTGTGAGTACCCTGC
CAGATTCCATGGATACACGT

Downstream 100 bases:

>100_bases
CGGCGGCGGCGTTGCGTGCGTTATACGCAGCAGCGCCACAATCCGGCGGGCGTCTTGGGGCACATTTTGCGTTTTGCCCC
GAGACGCATTACAATACGAG

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSADANAAAPRQDGAGEGEESSAPRRKGLRRGLRNLVAARRQQHEGRAESEPAGEGAASAGEGAQAPRKARAPRSRKPKE
ANAAAGEVVAIPEAMSLSAEGRPSAAEASAERRGARGRFGKGRRQAEGQPAGDRAEGQGGNAGGGNGGRGKNARPTRGAK
GQGKAAGQSRGKGGKGAEDVFQYVISGAYDADADVGGAPQSTKVRELTAEDDAPKLHKILADAGLGSRRDMEDLILQGRV
SVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGRLDFN
TEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELPESDRQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGR
NREVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLKVPSASASGGQGSQGGRQGNRRREAQ
PVLMGPMSSGFTGEATFQSRGQGIENGNRAMPAPRRQSNPRQPDPMQTSMGYINVGGPTTLTARTRMGGGRGLPGAGSSN
SGGSGNGNRAPGNKRPAKGGGGLPNGNKARGGGKRGKR

Sequences:

>Translated_598_residues
MSADANAAAPRQDGAGEGEESSAPRRKGLRRGLRNLVAARRQQHEGRAESEPAGEGAASAGEGAQAPRKARAPRSRKPKE
ANAAAGEVVAIPEAMSLSAEGRPSAAEASAERRGARGRFGKGRRQAEGQPAGDRAEGQGGNAGGGNGGRGKNARPTRGAK
GQGKAAGQSRGKGGKGAEDVFQYVISGAYDADADVGGAPQSTKVRELTAEDDAPKLHKILADAGLGSRRDMEDLILQGRV
SVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGRLDFN
TEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELPESDRQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGR
NREVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLKVPSASASGGQGSQGGRQGNRRREAQ
PVLMGPMSSGFTGEATFQSRGQGIENGNRAMPAPRRQSNPRQPDPMQTSMGYINVGGPTTLTARTRMGGGRGLPGAGSSN
SGGSGNGNRAPGNKRPAKGGGGLPNGNKARGGGKRGKR
>Mature_597_residues
SADANAAAPRQDGAGEGEESSAPRRKGLRRGLRNLVAARRQQHEGRAESEPAGEGAASAGEGAQAPRKARAPRSRKPKEA
NAAAGEVVAIPEAMSLSAEGRPSAAEASAERRGARGRFGKGRRQAEGQPAGDRAEGQGGNAGGGNGGRGKNARPTRGAKG
QGKAAGQSRGKGGKGAEDVFQYVISGAYDADADVGGAPQSTKVRELTAEDDAPKLHKILADAGLGSRRDMEDLILQGRVS
VNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGRLDFNT
EGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELPESDRQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGRN
REVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLKVPSASASGGQGSQGGRQGNRRREAQP
VLMGPMSSGFTGEATFQSRGQGIENGNRAMPAPRRQSNPRQPDPMQTSMGYINVGGPTTLTARTRMGGGRGLPGAGSSNS
GGSGNGNRAPGNKRPAKGGGGLPNGNKARGGGKRGKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=270, Percent_Identity=48.8888888888889, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788510, Length=214, Percent_Identity=28.0373831775701, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=30.9278350515464, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 62933; Mature: 62802

Theoretical pI: Translated: 11.47; Mature: 11.47

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADANAAAPRQDGAGEGEESSAPRRKGLRRGLRNLVAARRQQHEGRAESEPAGEGAASA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GEGAQAPRKARAPRSRKPKEANAAAGEVVAIPEAMSLSAEGRPSAAEASAERRGARGRFG
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHCCCCCCCC
KGRRQAEGQPAGDRAEGQGGNAGGGNGGRGKNARPTRGAKGQGKAAGQSRGKGGKGAEDV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FQYVISGAYDADADVGGAPQSTKVRELTAEDDAPKLHKILADAGLGSRRDMEDLILQGRV
HHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
SVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQSDPEGRPTVF
CCCCCHHHHCCCCCCCCCEEECHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCHHH
DSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELPESD
HCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCHHH
RQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGRNREVRRMFEAVNLTVSRLIR
HHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLKVPSASASGGQGSQGGRQGNRRREAQ
HHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
PVLMGPMSSGFTGEATFQSRGQGIENGNRAMPAPRRQSNPRQPDPMQTSMGYINVGGPTT
CEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCCCEE
LTARTRMGGGRGLPGAGSSNSGGSGNGNRAPGNKRPAKGGGGLPNGNKARGGGKRGKR
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SADANAAAPRQDGAGEGEESSAPRRKGLRRGLRNLVAARRQQHEGRAESEPAGEGAASA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GEGAQAPRKARAPRSRKPKEANAAAGEVVAIPEAMSLSAEGRPSAAEASAERRGARGRFG
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHCCCCCCCC
KGRRQAEGQPAGDRAEGQGGNAGGGNGGRGKNARPTRGAKGQGKAAGQSRGKGGKGAEDV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FQYVISGAYDADADVGGAPQSTKVRELTAEDDAPKLHKILADAGLGSRRDMEDLILQGRV
HHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
SVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQSDPEGRPTVF
CCCCCHHHHCCCCCCCCCEEECHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCHHH
DSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELPESD
HCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCHHH
RQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGRNREVRRMFEAVNLTVSRLIR
HHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLKVPSASASGGQGSQGGRQGNRRREAQ
HHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
PVLMGPMSSGFTGEATFQSRGQGIENGNRAMPAPRRQSNPRQPDPMQTSMGYINVGGPTT
CEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCCCEE
LTARTRMGGGRGLPGAGSSNSGGSGNGNRAPGNKRPAKGGGGLPNGNKARGGGKRGKR
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]