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The map label for this gene is rlmB

Identifier: 17545948

GI number: 17545948

Start: 1300833

End: 1301582

Strand: Direct

Name: rlmB

Synonym: RSc1229

Alternate gene names: 17545948

Gene position: 1300833-1301582 (Clockwise)

Preceding gene: 17545947

Following gene: 17545949

Centisome position: 35.0

GC content: 68.4

Gene sequence:

>750_bases
ATGTCCAAGTCCAAGCTCCTGATCGGCTTTCACGCCGTTACCGCCCGGCTGCGCCACGATGCGCGGTCGATCGATGAAAT
CTACTTCGAGGCCAACCGCCGTGACCGCCGCATGCAGGATTTCCTGAAAGCGGCGGAAGCCGCGGGCGTGCGCATGATTC
CGGCCGACAACGAACGGCTGCGCGGCATTGCCGGCACCGACCGCCACCAGGGCGTGGTGGCCAAGGCCGAGGCGCTGTCG
CTGGCGCTCAACCTCGACGAGCTGCTCGACGGCATCGAAGGTCCGCCGCTGCTGCTGGTGCTCGACGGCGTGACCGACCC
GCACAACCTCGGCGCTTGCCTGCGCGTGGCCGATGCGGCCGGCGCGCACGCGGTGATCGCGCCGAAGGACCGCAGCGTCG
GCATCAATACCACTGTCGCCAAGGTGGCGAGCGGTGCGGCCGAGACCGTTCCCTACATCACCGTGACCAACCTGGCGCGC
ACGCTGCGCGAGCTGCAGCAGCGCGGTGTCTGGGTGATCGGCACGGCTGACGAGGCAGAGCAGGATCTCTATCGGGCTGA
CTTCAAGGGCCCCATCGCGCTGGTGATGGGCGCGGAAGGGGAAGGCATGCGCCGCCTCACGCGCGAGACCTGCGACACGC
TGGTCAGCATCCCGATGGCGGGCAGTGTGGAGAGCCTGAACGTCTCGGTGGCCAGCGGTGTGTGCCTGTTCGAGGCGGTG
CGCCAGCGCAGCGTCGTGCCTGCCAAGTGA

Upstream 100 bases:

>100_bases
AAGCCCAAGACCGGCGGCAAGACCGCGACGCGCAAGGCGCCGGGCAAGACCGTGCGGTCGCGCAAGGGGCGGTGAGCAGC
GGGGCGGGTACAATCGCGCC

Downstream 100 bases:

>100_bases
TGGACGTATTGCCCACGGCTGTCTCCGACGCGGCGGCCCCGGCTCAGGCGCGTGCCTGGATCGAGGCGGCCGAGCGCGTC
ATGGTGCTGACCGGCGCCGG

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSKSKLLIGFHAVTARLRHDARSIDEIYFEANRRDRRMQDFLKAAEAAGVRMIPADNERLRGIAGTDRHQGVVAKAEALS
LALNLDELLDGIEGPPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLAR
TLRELQQRGVWVIGTADEAEQDLYRADFKGPIALVMGAEGEGMRRLTRETCDTLVSIPMAGSVESLNVSVASGVCLFEAV
RQRSVVPAK

Sequences:

>Translated_249_residues
MSKSKLLIGFHAVTARLRHDARSIDEIYFEANRRDRRMQDFLKAAEAAGVRMIPADNERLRGIAGTDRHQGVVAKAEALS
LALNLDELLDGIEGPPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLAR
TLRELQQRGVWVIGTADEAEQDLYRADFKGPIALVMGAEGEGMRRLTRETCDTLVSIPMAGSVESLNVSVASGVCLFEAV
RQRSVVPAK
>Mature_248_residues
SKSKLLIGFHAVTARLRHDARSIDEIYFEANRRDRRMQDFLKAAEAAGVRMIPADNERLRGIAGTDRHQGVVAKAEALSL
ALNLDELLDGIEGPPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLART
LRELQQRGVWVIGTADEAEQDLYRADFKGPIALVMGAEGEGMRRLTRETCDTLVSIPMAGSVESLNVSVASGVCLFEAVR
QRSVVPAK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=267, Percent_Identity=27.7153558052434, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=54.7325102880658, Blast_Score=257, Evalue=5e-70,
Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=35.0649350649351, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788935, Length=187, Percent_Identity=29.9465240641711, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_RALSO (Q8Y016)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519350.1
- ProteinModelPortal:   Q8Y016
- SMR:   Q8Y016
- GeneID:   1220052
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1229
- NMPDR:   fig|267608.1.peg.1229
- HOGENOM:   HBG714057
- OMA:   LIGFHAV
- ProtClustDB:   CLSK896883
- BioCyc:   RSOL267608:RSC1229-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26690; Mature: 26559

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 197-197 BINDING 217-217 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSKLLIGFHAVTARLRHDARSIDEIYFEANRRDRRMQDFLKAAEAAGVRMIPADNERL
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCHHH
RGIAGTDRHQGVVAKAEALSLALNLDELLDGIEGPPLLLVLDGVTDPHNLGACLRVADAA
CCCCCCCCCCCEEEHHHHHHHEECHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCC
GAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLARTLRELQQRGVWVIGTADEAE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
QDLYRADFKGPIALVMGAEGEGMRRLTRETCDTLVSIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHH
RQRSVVPAK
HHCCCCCCC
>Mature Secondary Structure 
SKSKLLIGFHAVTARLRHDARSIDEIYFEANRRDRRMQDFLKAAEAAGVRMIPADNERL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCHHH
RGIAGTDRHQGVVAKAEALSLALNLDELLDGIEGPPLLLVLDGVTDPHNLGACLRVADAA
CCCCCCCCCCCEEEHHHHHHHEECHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCC
GAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLARTLRELQQRGVWVIGTADEAE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
QDLYRADFKGPIALVMGAEGEGMRRLTRETCDTLVSIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHH
RQRSVVPAK
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852