Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 17545945

GI number: 17545945

Start: 1295663

End: 1297003

Strand: Direct

Name: purA [H]

Synonym: RSc1226

Alternate gene names: 17545945

Gene position: 1295663-1297003 (Clockwise)

Preceding gene: 17545944

Following gene: 17545946

Centisome position: 34.86

GC content: 65.92

Gene sequence:

>1341_bases
ATGTCCGCACCAGCAGTTAGCCAGGGACGCAATGTCGTCGTCATCGGCACCCAGTGGGGTGACGAGGGTAAAGGGAAAAT
CGTCGATTGGCTGACCGATCACGCGCAAGGCGTGGTTCGCTTCCAGGGCGGACACAACGCAGGCCACACCCTGATCATCG
GCGGCAAGAAGACCATTCTGCGGCTGATCCCGTCGGGCATCATGCGCGATGGCGTGGCGTGCTACATCGGTAACGGCGTG
GTGCTGTCGCCCGAGGCGCTGTTCAAGGAAATCGACGAGCTGGAGTCGGCCGGCGTGGAGGTCAAGAGCCGCCTGCGGAT
CTCCGAGGCGACCACGCTGATCCTGCCGTATCACATCGCCATCGATAAGGCGCGCGAGATCAAGCGCGGTGCCGCCAAGA
TCGGCACCACCGGCCGCGGCATCGGTCCGGCGTACGAAGACAAGGTCGCTCGCCGCGCGCTGCGCGTGCAGGACCTGTTC
GACCCGGCCTACTTTGCCGAGCGCCTGCGCGAGAACCTCGATTTCCACAACTTCGTGCTGACACAGTATCTGAACCATCC
GGCGCTGGATTTCCAGCAGACGCTGGACGAGATGCTGCCGTACGGCGAGCGCCTCGCGCCGATGGTTACCGACGTGTCGG
CCGAGCTGTTTGCGGCCAACGCCGCCGGCAAGAACCTCATGTTCGAAGGCGCGCAGGGCACGCTGCTCGACATCGACCAC
GGCACCTATCCGTTCGTCACCTCCAGCAACTGCGTGGCCGGCAACGCGGCCGCCGGTGCGGGCGTGGGGCCGGGTCAGCT
GCACTACATCCTGGGGATCACCAAGGCCTACTGCACGCGCGTCGGCTCCGGCCCGTTTCCGAGCGAGCTGTATGACGCCG
ACAACCCCGCGCGCCAGGACCCGACCGGCGTGCGCCTGGCCAACGTGGGCAAGGAATTCGGCTCGGTCACGGGCCGTCCG
CGCCGCACCGGCTGGCTGGATGCCGCCGCGCTGCGCCGCGCGATCCAGATCAACGGCGTGTCGGGCCTGTGCATGACCAA
GCTGGACGTACTCGACGGCCTCGAGACCATCAAGTTGTGCGTCGGCTACATGCTGGACGGCAAGTCGATCGACATCCTGC
CGCGCGGCTCGGATGCCGTGGCGCGCTGCCAGCCGGTGTACGAGGAATTCCCGGGCTGGAACACGTCGACCTTCGGTCTG
AAGGAGTGGGATGCGCTGCCGGAAACCGCGCAGGCCTACCTGAAGCGGGTGGAAGAAGTAGCCGGCATCCCGATCGCCAT
GATCTCGACCGGTCCGGATCGCGACGAGACCATCCTGCTGCGTCACCCGTACAAGGATTGA

Upstream 100 bases:

>100_bases
CTGAGGCTGTTACACGAACGCAGTATGAGCCTCCGCTCGTTTCGGGGACCGCCCTGACAACGAGGGTAGAATACGTTTTT
AACCCATCAGTAAATTCAAC

Downstream 100 bases:

>100_bases
TGCGCGCGCGGCGGCTCTCGGCGCCATGCGGTTTTCGACTCGCCCGGTGATTCGCCGGGCGAATTCGCATGGGGCGCCGC
ATCCGGCGGAACATGACAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGV
VLSPEALFKEIDELESAGVEVKSRLRISEATTLILPYHIAIDKAREIKRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLPYGERLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPTGVRLANVGKEFGSVTGRP
RRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETIKLCVGYMLDGKSIDILPRGSDAVARCQPVYEEFPGWNTSTFGL
KEWDALPETAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD

Sequences:

>Translated_446_residues
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGV
VLSPEALFKEIDELESAGVEVKSRLRISEATTLILPYHIAIDKAREIKRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLPYGERLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPTGVRLANVGKEFGSVTGRP
RRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETIKLCVGYMLDGKSIDILPRGSDAVARCQPVYEEFPGWNTSTFGL
KEWDALPETAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD
>Mature_445_residues
SAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGVV
LSPEALFKEIDELESAGVEVKSRLRISEATTLILPYHIAIDKAREIKRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFD
PAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLPYGERLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDHG
TYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPTGVRLANVGKEFGSVTGRPR
RTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETIKLCVGYMLDGKSIDILPRGSDAVARCQPVYEEFPGWNTSTFGLK
EWDALPETAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=444, Percent_Identity=40.3153153153153, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=40.0452488687783, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=38.5185185185185, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=59.7254004576659, Blast_Score=533, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=40.5466970387244, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=439, Percent_Identity=40.5466970387244, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=38.2151029748284, Blast_Score=311, Evalue=2e-85,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=38.0510440835267, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48266; Mature: 48134

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRDGVACYIGNGVVLSPEALFKEIDELESAGVEVKSRLRISEATTLILPYHIA
HHHHHHHHHCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEECCCEEEEEEEEEH
IDKAREIKRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPAYFAERLRENLDFHNFVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
TQYLNHPALDFQQTLDEMLPYGERLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQD
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
PTGVRLANVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETIKLC
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGYMLDGKSIDILPRGSDAVARCQPVYEEFPGWNTSTFGLKEWDALPETAQAYLKRVEEV
HHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
AGIPIAMISTGPDRDETILLRHPYKD
CCCCEEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRDGVACYIGNGVVLSPEALFKEIDELESAGVEVKSRLRISEATTLILPYHIA
HHHHHHHHHCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEECCCEEEEEEEEEH
IDKAREIKRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPAYFAERLRENLDFHNFVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
TQYLNHPALDFQQTLDEMLPYGERLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQD
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
PTGVRLANVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETIKLC
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGYMLDGKSIDILPRGSDAVARCQPVYEEFPGWNTSTFGLKEWDALPETAQAYLKRVEEV
HHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
AGIPIAMISTGPDRDETILLRHPYKD
CCCCEEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA