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The map label for this gene is nagI

Identifier: 17545806

GI number: 17545806

Start: 1145035

End: 1146081

Strand: Reverse

Name: nagI

Synonym: RSc1087

Alternate gene names: NA

Gene position: 1146081-1145035 (Counterclockwise)

Preceding gene: 17545807

Following gene: 17545805

Centisome position: 30.84

GC content: 70.3

Gene sequence:

>1047_bases
ATGACTTCGCAGACACTGCCGCCTGAGCGCGACACCTACTACAGCCTGCTCGGCGGGATGGGCCTGGCGCCGCTCTGGGA
GTCGCTCCATGCGCTCGTTCCGCGCGAGCCGAAGCCGCGCATCGTGCCGGCGCACTGGCAATACGCGGCGATCCGCCCGC
TGGTGATGCAGGCCGGCCGCATCATCAGCGCCGAAGAGGCCGTGCGCCGCGTGCTGGTGCTGGAGAACCCGGGACTGCCC
GGCCGCTCCAGCATCACCTCCACGCTGTATGCCGGCCTGCAGCTGATCCTGCCGGGCGAGATCGCGCCCAGCCACCGCCA
TACGCAGTCGGCGCTGCGCTTCATCGTCGAGGGCAAGGGCGCGTGGACGGCCGTCGACGGCGAGCGCACCACCATGCATC
CGGGCGATTTCATCATCACGCCGTCCTGGACCTGGCACGACCACGGCTGCCCGCGCCTCGACGAAGGCGGCGAGCCGGTG
GTGTGGCTCGACGGCCTGGACATTCCGCTGGTGCAGCAGTTCGATGCGGGCTTCGCCGAGAACCACCCCGAAGCGCAGCA
GCCGGTGACGAGGCCCGAGGGCGACAGCTTCGCGCGCTTCGGCCACAACATGGTGCCGGTGCGGCACCGGGTGAGCGATC
CCACCTCGCCGGTCTTCAGCTACCCGTACGAACGCTCGCGCGAAGCGCTGGACACGCTCTACCGCCACGGCGAGCTCGAC
CCATGGGACGGTGTCAAGCTGCGCTACGTCAACCCGGCCACCGGCGGCTGGCCGATGCCGACCATCGCCACCTTCATGCA
GTACCTGCCGGCGGGCTTCCGGGGCAAGACGTATCGCAGCACCGATGCCACCGTCTACTGCGTGGTGGAAGGCCGCGGCA
CGGCGCACATCGGCGAGGCACGGTTCGCGTTCGCGCCGCACGACGTGTTCGTCGTGCCGTCGTGGGCACCGGTGCGCCTC
GACGCGCAGGACGACGCCGTGCTGTTCAGCTATTCCGACCGGCCCGTGCTGGCCGCGCTCAACCTGCTGCGCGAGGCGCG
CACCTGA

Upstream 100 bases:

>100_bases
TCGGGCAAGGCGATGTGCGAGCCGCTGAGCCGCGACGTGCGGACGTATCCGATCAAGATCGAAGACGGCCACGTCTTCGT
CGAACTGTGAGGGCCGCGCC

Downstream 100 bases:

>100_bases
CCCCGGCCGCTCCCGATTGCCGACGCGGCGTGCCGGCCGCCGCCGGCCCCGCTCACCCTTCCGATCCGATCCGATCCGAT
CCGATCCGATCCGAACCGAC

Product: gentisate 1,2-dioxygenase oxidoreductase

Products: maleylpyruvate

Alternate protein names: Cupin 2 Domain-Containing Protein; Cupin 2 Conserved Barrel Domain Protein; Cupin 2 Barrel; Cupin 2 Conserved Barrel Domain-Containing Protein; 1 2-Dioxygenase; Gentisate 1 2-Dioxygenase Oxidoreductase Protein; Cupin 2 Protein; 1-Hydroxy-2-Naphthoate Dioxygenase; Gentisate 1 2-Dioxygenase Oxidoreductase; Dioxygenase Oxidoreductase Protein; Dioxygenase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGRIISAEEAVRRVLVLENPGLP
GRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKGAWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPV
VWLDGLDIPLVQQFDAGFAENHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELD
PWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEARFAFAPHDVFVVPSWAPVRL
DAQDDAVLFSYSDRPVLAALNLLREART

Sequences:

>Translated_348_residues
MTSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGRIISAEEAVRRVLVLENPGLP
GRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKGAWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPV
VWLDGLDIPLVQQFDAGFAENHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELD
PWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEARFAFAPHDVFVVPSWAPVRL
DAQDDAVLFSYSDRPVLAALNLLREART
>Mature_347_residues
TSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGRIISAEEAVRRVLVLENPGLPG
RSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKGAWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPVV
WLDGLDIPLVQQFDAGFAENHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELDP
WDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEARFAFAPHDVFVVPSWAPVRLD
AQDDAVLFSYSDRPVLAALNLLREART

Specific function: Unknown

COG id: COG3435

COG function: function code Q; Gentisate 1,2-dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.13.11.4

Molecular weight: Translated: 38719; Mature: 38588

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGR
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCC
IISAEEAVRRVLVLENPGLPGRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKG
EECHHHHHHEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHEEEECCC
AWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPVVWLDGLDIPLVQQFDAGFAE
CEEEECCCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCC
NHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELD
CCCCHHCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
PWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEA
CCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEECCC
RFAFAPHDVFVVPSWAPVRLDAQDDAVLFSYSDRPVLAALNLLREART
EEEECCCCEEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGR
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCC
IISAEEAVRRVLVLENPGLPGRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKG
EECHHHHHHEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHEEEECCC
AWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPVVWLDGLDIPLVQQFDAGFAE
CEEEECCCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCC
NHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELD
CCCCHHCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
PWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEA
CCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEECCC
RFAFAPHDVFVVPSWAPVRLDAQDDAVLFSYSDRPVLAALNLLREART
EEEECCCCEEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-dihydroxybenzoate; O2

Specific reaction: 2,5-dihydroxybenzoate + O2 = maleylpyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA