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The map label for this gene is cht60 [H]

Identifier: 17545488

GI number: 17545488

Start: 810521

End: 812725

Strand: Direct

Name: cht60 [H]

Synonym: RSc0769

Alternate gene names: 17545488

Gene position: 810521-812725 (Clockwise)

Preceding gene: 17545487

Following gene: 17545489

Centisome position: 21.81

GC content: 66.94

Gene sequence:

>2205_bases
ATGCCAATCGACGAACTCAAACTTGCCAAGATGCCGGGGCATCCCCCGGATGTGCGCGGCGATCGGGCTGACGGCGGTCC
AATCCGTTCTGGCCGCAGCCCCGGATGGCTGGCCCGGCGCTCCATCCGGCCGAGACTGGCGATGAAGGTGTTGCGCGTTC
TTCGCCAGTGCGTGCTGGGTCTTGCGGTGCTCTCGTTGGCCGCGTGCTCGTCTGATGATGAAACCGCAAATGACGCGCAG
TTGAAGGCGCAGGCACTGCAGATCGTCGGGCAGATGTCGACGCGCGACAAGATCGGGCAAAAGATCATGATGGCGTTCCG
CTACTGGTGCGAGCCGCCTGCCGATCCCAACTGCACGGATCGCATGACGGTGCTGCCCGATGCTGCCGCCAGCGCGTTGC
GCGATCACCGCATCGGCGGCGTGGTCCTGTTCGCGAACAACCTCGTATCGGTCGATCAGATTAGCCGCCTGACAGCGCAG
ATCGCTGCGGCGGCGCCGGTCGGCATGCTGATCGGCGTCGACGAGGAAGGCGGCAACGTGTTCCGGTTGCCGCGTGGCAT
CGCGACGTCGTTTCCCGGGAACATGGCGCTCGGAGCGGCATACCTGGGCGCCGCCGATGCGGCACTTGCCCACGATGAGG
GACGCGTGCTCGCTTCCGAACTGGCGGCGGTCGGCATGAATGTCAACTTTGCGCCGGTGGTCGACGTCAACAGCAATCCG
CTGAATCCCGTCATCAATGTGCGCGCCTACGGCGATGATCCGGCCATGGTCGGCTTGCTCGGCCGCCACATGGCGCGCGG
CATGGCTCACGCGGGCGTCATCAGCGCATTCAAGCACTTCCCGGGCCACGGGGATGCGGCAACCGATTCGCACTATGGGC
TGCCGCGCGTGGACAAGTCGCGTGCCGACGCGTACGCGACCGACCTGGCGCCGTATCGCGCATCGATTGACCTGGGCGAA
GCGCCGGAGATGATCATGACCGCGCATATCCAGTATCCGTCGCTTGACGAAACCCGGGTCGCGACGCGCACCGGCGAGGG
CATCCTGGTGCCGGCCACCATGTCACGCAAGATCCAGCACGACCTGCTGCGCGGCGAGTTCGGCTACCGCGGCGTGACGG
TCTCCGATGCGCTCGACATGGATGCGATCGCAAACCACTTCGATCCCGATCAGGCGGTGATCAACCTGTTTCGCGCCGAC
GTCGACATCGCGCTGATGCCCGTCGAGTTCCGCACGGCCGGTCAGGCCGATCGGCTCCGTGCGGTGATCGACCGCGTCGT
GGCCGCGTTGGATGCCGGCACGCTGAGTCGCGCCGAGCTGGATGCATCGGTCGAGCGCATCACACTGATGAAGCTGCGCC
ACGGGATCGCACCGAACGCATCGCGCCCGCAGACATCTGCGGTAACGGTGATCGGCAGCCCCGGCCATCGGGCGGCCGAG
CAGCGCATTGCGCAGGCATCGATCACGCTCTTGCGCAATCGCGGCGCGACGCTGCCGCTGAACGCGCCGCAACGGCCGAT
CTTCATCATGACGCCCTGGGGCGAGCAGGCCCAGGGGATGCGCCAGCGCTTCGCTGAACTCGGCTACGCTTCGGTGACCG
GCGCGAAGCTGGCGGAGACGCCCTGGGCCGAGCAGCAACGGTTGATCGATGCCGCCGATATCGTGATTCTCGGGACCCTG
TCGAGCGCCCCCTCTCCGGTCGAGCGCAATGGCGATCCGCGCGCCGCCGACACGCGGACGAATACCGCCTCGGTGCAAGA
GGGTGCTGCGCAGCCCGTCGGCGACAAGCGCCCCGAGGCCGTTGCGCACGGGCAACGCTCCCTGGTCTTCGATGTCGAGG
GGGACGCGCCGACCGTGCCCGGGGTGCTGCGCGCGCAACAGGTCTCCGCGCCAAGCGAAGCGCAGCGGGTCCGCTATGCG
ATGGAGTACGCCAAGGCGAAGGGCAAGACCGTCATCCACGTATCACTGCGCGCGCCGTATGACGTGCCTTCGTTCGACGA
TGTCGCTGACGCGACGGTTGCCACCTATTCGTACTACGGCTACGACAACGGCGCCTGGCGCGGTCCGTCGATGCCGGCAC
TGGTCGATGCGCTGGCCGGTGTGCGCTCGCCGCTTGGCCGCCTGCCGGTGTCGGTGTATGAGGTGAGCATGGACGGCAAG
CTGGGTTCACTGCGTTACGCGCGCGGCTTCGGGATGCGCTACTGA

Upstream 100 bases:

>100_bases
GGCACCTTCGCTTGCCGTCGACGCGCGCAGTGGCCCGTGTCCGGCGTACCCCGGCTATCCGCCGTTCCTTTCTATAACTC
CGACCCAACCAAGCGAGGCA

Downstream 100 bases:

>100_bases
GCGCGGCCGGAAGCACCGTTTCAGTTCGCGGTGGGGCGAGCGCGGGGCCGGTTGCCTGACGGAAAGACGATCGGCCGCGC
AGTGCGGCAGGCATTGCGAT

Product: hydrolase glycosidase

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MPIDELKLAKMPGHPPDVRGDRADGGPIRSGRSPGWLARRSIRPRLAMKVLRVLRQCVLGLAVLSLAACSSDDETANDAQ
LKAQALQIVGQMSTRDKIGQKIMMAFRYWCEPPADPNCTDRMTVLPDAAASALRDHRIGGVVLFANNLVSVDQISRLTAQ
IAAAAPVGMLIGVDEEGGNVFRLPRGIATSFPGNMALGAAYLGAADAALAHDEGRVLASELAAVGMNVNFAPVVDVNSNP
LNPVINVRAYGDDPAMVGLLGRHMARGMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKSRADAYATDLAPYRASIDLGE
APEMIMTAHIQYPSLDETRVATRTGEGILVPATMSRKIQHDLLRGEFGYRGVTVSDALDMDAIANHFDPDQAVINLFRAD
VDIALMPVEFRTAGQADRLRAVIDRVVAALDAGTLSRAELDASVERITLMKLRHGIAPNASRPQTSAVTVIGSPGHRAAE
QRIAQASITLLRNRGATLPLNAPQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAETPWAEQQRLIDAADIVILGTL
SSAPSPVERNGDPRAADTRTNTASVQEGAAQPVGDKRPEAVAHGQRSLVFDVEGDAPTVPGVLRAQQVSAPSEAQRVRYA
MEYAKAKGKTVIHVSLRAPYDVPSFDDVADATVATYSYYGYDNGAWRGPSMPALVDALAGVRSPLGRLPVSVYEVSMDGK
LGSLRYARGFGMRY

Sequences:

>Translated_734_residues
MPIDELKLAKMPGHPPDVRGDRADGGPIRSGRSPGWLARRSIRPRLAMKVLRVLRQCVLGLAVLSLAACSSDDETANDAQ
LKAQALQIVGQMSTRDKIGQKIMMAFRYWCEPPADPNCTDRMTVLPDAAASALRDHRIGGVVLFANNLVSVDQISRLTAQ
IAAAAPVGMLIGVDEEGGNVFRLPRGIATSFPGNMALGAAYLGAADAALAHDEGRVLASELAAVGMNVNFAPVVDVNSNP
LNPVINVRAYGDDPAMVGLLGRHMARGMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKSRADAYATDLAPYRASIDLGE
APEMIMTAHIQYPSLDETRVATRTGEGILVPATMSRKIQHDLLRGEFGYRGVTVSDALDMDAIANHFDPDQAVINLFRAD
VDIALMPVEFRTAGQADRLRAVIDRVVAALDAGTLSRAELDASVERITLMKLRHGIAPNASRPQTSAVTVIGSPGHRAAE
QRIAQASITLLRNRGATLPLNAPQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAETPWAEQQRLIDAADIVILGTL
SSAPSPVERNGDPRAADTRTNTASVQEGAAQPVGDKRPEAVAHGQRSLVFDVEGDAPTVPGVLRAQQVSAPSEAQRVRYA
MEYAKAKGKTVIHVSLRAPYDVPSFDDVADATVATYSYYGYDNGAWRGPSMPALVDALAGVRSPLGRLPVSVYEVSMDGK
LGSLRYARGFGMRY
>Mature_733_residues
PIDELKLAKMPGHPPDVRGDRADGGPIRSGRSPGWLARRSIRPRLAMKVLRVLRQCVLGLAVLSLAACSSDDETANDAQL
KAQALQIVGQMSTRDKIGQKIMMAFRYWCEPPADPNCTDRMTVLPDAAASALRDHRIGGVVLFANNLVSVDQISRLTAQI
AAAAPVGMLIGVDEEGGNVFRLPRGIATSFPGNMALGAAYLGAADAALAHDEGRVLASELAAVGMNVNFAPVVDVNSNPL
NPVINVRAYGDDPAMVGLLGRHMARGMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKSRADAYATDLAPYRASIDLGEA
PEMIMTAHIQYPSLDETRVATRTGEGILVPATMSRKIQHDLLRGEFGYRGVTVSDALDMDAIANHFDPDQAVINLFRADV
DIALMPVEFRTAGQADRLRAVIDRVVAALDAGTLSRAELDASVERITLMKLRHGIAPNASRPQTSAVTVIGSPGHRAAEQ
RIAQASITLLRNRGATLPLNAPQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAETPWAEQQRLIDAADIVILGTLS
SAPSPVERNGDPRAADTRTNTASVQEGAAQPVGDKRPEAVAHGQRSLVFDVEGDAPTVPGVLRAQQVSAPSEAQRVRYAM
EYAKAKGKTVIHVSLRAPYDVPSFDDVADATVATYSYYGYDNGAWRGPSMPALVDALAGVRSPLGRLPVSVYEVSMDGKL
GSLRYARGFGMRY

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=308, Percent_Identity=30.5194805194805, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1788453, Length=692, Percent_Identity=23.2658959537572, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 78454; Mature: 78323

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00775 GLYCOSYL_HYDROL_F3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDELKLAKMPGHPPDVRGDRADGGPIRSGRSPGWLARRSIRPRLAMKVLRVLRQCVLG
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
LAVLSLAACSSDDETANDAQLKAQALQIVGQMSTRDKIGQKIMMAFRYWCEPPADPNCTD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RMTVLPDAAASALRDHRIGGVVLFANNLVSVDQISRLTAQIAAAAPVGMLIGVDEEGGNV
CEEECCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCE
FRLPRGIATSFPGNMALGAAYLGAADAALAHDEGRVLASELAAVGMNVNFAPVVDVNSNP
EECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCC
LNPVINVRAYGDDPAMVGLLGRHMARGMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RADAYATDLAPYRASIDLGEAPEMIMTAHIQYPSLDETRVATRTGEGILVPATMSRKIQH
HHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHEECCCCEEEEHHHHHHHHH
DLLRGEFGYRGVTVSDALDMDAIANHFDPDQAVINLFRADVDIALMPVEFRTAGQADRLR
HHHHCCCCCCCEEHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
AVIDRVVAALDAGTLSRAELDASVERITLMKLRHGIAPNASRPQTSAVTVIGSPGHRAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
QRIAQASITLLRNRGATLPLNAPQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAET
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHCC
PWAEQQRLIDAADIVILGTLSSAPSPVERNGDPRAADTRTNTASVQEGAAQPVGDKRPEA
CCHHHHHHHHHCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHH
VAHGQRSLVFDVEGDAPTVPGVLRAQQVSAPSEAQRVRYAMEYAKAKGKTVIHVSLRAPY
HHCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DVPSFDDVADATVATYSYYGYDNGAWRGPSMPALVDALAGVRSPLGRLPVSVYEVSMDGK
CCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEEEEEEECCC
LGSLRYARGFGMRY
CCCEEHHCCCCCCC
>Mature Secondary Structure 
PIDELKLAKMPGHPPDVRGDRADGGPIRSGRSPGWLARRSIRPRLAMKVLRVLRQCVLG
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
LAVLSLAACSSDDETANDAQLKAQALQIVGQMSTRDKIGQKIMMAFRYWCEPPADPNCTD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RMTVLPDAAASALRDHRIGGVVLFANNLVSVDQISRLTAQIAAAAPVGMLIGVDEEGGNV
CEEECCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCE
FRLPRGIATSFPGNMALGAAYLGAADAALAHDEGRVLASELAAVGMNVNFAPVVDVNSNP
EECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCC
LNPVINVRAYGDDPAMVGLLGRHMARGMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RADAYATDLAPYRASIDLGEAPEMIMTAHIQYPSLDETRVATRTGEGILVPATMSRKIQH
HHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHEECCCCEEEEHHHHHHHHH
DLLRGEFGYRGVTVSDALDMDAIANHFDPDQAVINLFRADVDIALMPVEFRTAGQADRLR
HHHHCCCCCCCEEHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
AVIDRVVAALDAGTLSRAELDASVERITLMKLRHGIAPNASRPQTSAVTVIGSPGHRAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
QRIAQASITLLRNRGATLPLNAPQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAET
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHCC
PWAEQQRLIDAADIVILGTLSSAPSPVERNGDPRAADTRTNTASVQEGAAQPVGDKRPEA
CCHHHHHHHHHCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHH
VAHGQRSLVFDVEGDAPTVPGVLRAQQVSAPSEAQRVRYAMEYAKAKGKTVIHVSLRAPY
HHCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DVPSFDDVADATVATYSYYGYDNGAWRGPSMPALVDALAGVRSPLGRLPVSVYEVSMDGK
CCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEEEEEEECCC
LGSLRYARGFGMRY
CCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]