Click here to switch to the map view.

The map label for this gene is pyrX [H]

Identifier: 17545398

GI number: 17545398

Start: 726997

End: 728274

Strand: Direct

Name: pyrX [H]

Synonym: RSc0679

Alternate gene names: 17545398

Gene position: 726997-728274 (Clockwise)

Preceding gene: 17545397

Following gene: 17545399

Centisome position: 19.56

GC content: 69.72

Gene sequence:

>1278_bases
ATGAAACTGCATATCAAGGGCGGCCGCCTGATCGACCCGGCCAACGGCATCGACGCGCAACAGGATCTGTACCTCGCCGC
GGGCAAGGTGGTCGGCGTCGGCCGCGCGCCAGCGGATTTCCACGCCAACAAGACCATCGACGCCACCGGGCTGGTCGTCT
GTCCGGGGCTCATCGACCTGTCCGCCCGCCTGCGCGAGCCGGGCTACGAATATAAGGCGACGCTCGAATCGGAGATGGCC
GCCGCCATGGCCGGTGGCGTGACCTCGCTGGTGTGTCCGCCGGATACCGACCCCGTGCTTGACGAGCCCGGCCTGGTCGA
AATGCTCAAGTTCCGCGCCCGCAACCTGAACCAGGCGCATGTCTATCCGCTGGGCGCGCTGACGGTCGGCCTCAAGGGCC
TGGTGCTGACCGAGATGGCCGAGCTGACCGAAGCCGGCTGCGTGGGCTTCTCGCAGGCCGATGCGCCGATGACCGACACG
CTGGTGCTGATGCGTGCGCTGCAATACGCGCAGACCTTCGGCTTCACCGTCTGGCTGCGCCCGGAGGACCCGTTCCTGGG
CAAGGGCGGCGTGGCGGCGAGCGGCCCGCTGGCCTCGCGCCTGGGCCTGGCCGGCGTGCCGGTGATGGCCGAGACCGTGC
GCCTGCACACCATCTTCGAACTGATACGCAGCACCGGCGCGCGCGTGCACCTGTGCCGCCTGTCGTCGGCTGCCGGGCTG
GAGCTGGTGCGCCGTGCCAAGGCCGAGGGCCTGCCGGTCACCTGCGACGTCAATGTCCACCATATCTCGCTCACCGATGT
CGACATCGGCTACTTCAACTCGCAGATGCGCTTCATGCCGCCGCTGCGGGCCGGCCGCGATCGCGACGGCATCGTGCGCG
CGCTGGCCGACGGCACCATCGACGCCATCTGCTCCGACCACACGCCGGTGGATGACGATGAAAAGCTGCTGCCCTTCGCC
GAGGCCTCGCCCGGTGCGACCGGCCTCGAAACCCTGCTGCCGCTGACGCTGCGCTGGGCGGCGGAGCACAAGGTCGACCT
GCCGCGCGCGCTGGCGCGCATCACCAGCGAACCGGCGCGCGTGCTGGGCCTGCAGGCCGGCTCGCTGGAAGAGGGGGCGA
TGGCCGACGTGTGCGTGTTCGATCCGGACGCCACCTGGAAGGTCGAGCCGCGCAGCCTGCGCAGCCAGGGCAAGAACTCG
CCGTATCTCGGCTTTGAGCTGGCCGGCCGCGTGCGGGCCACGCTGGTTGCCGGTCATCTGGCCTATGACGGGCATTGA

Upstream 100 bases:

>100_bases
ACGGCGTGCAATCGGTGATCTTGAACCAGGTGACGTTCGGCATTGCCGTGCGGATGGCGGTGATGGGCATCGTGGCGGGC
AACAACGACTAAGAAGACCG

Downstream 100 bases:

>100_bases
TCAGCGCGCCGACGCGGCGCCCGCCGAGGTGGCCGTGCCGGCGCAGCGCAGGCATGTGCTGCGCAAGCTGCGCTTGGCCA
TCCACCTGATCGAGGGCATT

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLHIKGGRLIDPANGIDAQQDLYLAAGKVVGVGRAPADFHANKTIDATGLVVCPGLIDLSARLREPGYEYKATLESEMA
AAMAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNLNQAHVYPLGALTVGLKGLVLTEMAELTEAGCVGFSQADAPMTDT
LVLMRALQYAQTFGFTVWLRPEDPFLGKGGVAASGPLASRLGLAGVPVMAETVRLHTIFELIRSTGARVHLCRLSSAAGL
ELVRRAKAEGLPVTCDVNVHHISLTDVDIGYFNSQMRFMPPLRAGRDRDGIVRALADGTIDAICSDHTPVDDDEKLLPFA
EASPGATGLETLLPLTLRWAAEHKVDLPRALARITSEPARVLGLQAGSLEEGAMADVCVFDPDATWKVEPRSLRSQGKNS
PYLGFELAGRVRATLVAGHLAYDGH

Sequences:

>Translated_425_residues
MKLHIKGGRLIDPANGIDAQQDLYLAAGKVVGVGRAPADFHANKTIDATGLVVCPGLIDLSARLREPGYEYKATLESEMA
AAMAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNLNQAHVYPLGALTVGLKGLVLTEMAELTEAGCVGFSQADAPMTDT
LVLMRALQYAQTFGFTVWLRPEDPFLGKGGVAASGPLASRLGLAGVPVMAETVRLHTIFELIRSTGARVHLCRLSSAAGL
ELVRRAKAEGLPVTCDVNVHHISLTDVDIGYFNSQMRFMPPLRAGRDRDGIVRALADGTIDAICSDHTPVDDDEKLLPFA
EASPGATGLETLLPLTLRWAAEHKVDLPRALARITSEPARVLGLQAGSLEEGAMADVCVFDPDATWKVEPRSLRSQGKNS
PYLGFELAGRVRATLVAGHLAYDGH
>Mature_425_residues
MKLHIKGGRLIDPANGIDAQQDLYLAAGKVVGVGRAPADFHANKTIDATGLVVCPGLIDLSARLREPGYEYKATLESEMA
AAMAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNLNQAHVYPLGALTVGLKGLVLTEMAELTEAGCVGFSQADAPMTDT
LVLMRALQYAQTFGFTVWLRPEDPFLGKGGVAASGPLASRLGLAGVPVMAETVRLHTIFELIRSTGARVHLCRLSSAAGL
ELVRRAKAEGLPVTCDVNVHHISLTDVDIGYFNSQMRFMPPLRAGRDRDGIVRALADGTIDAICSDHTPVDDDEKLLPFA
EASPGATGLETLLPLTLRWAAEHKVDLPRALARITSEPARVLGLQAGSLEEGAMADVCVFDPDATWKVEPRSLRSQGKNS
PYLGFELAGRVRATLVAGHLAYDGH

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=394, Percent_Identity=28.1725888324873, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI19923821, Length=456, Percent_Identity=25.219298245614, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786722, Length=431, Percent_Identity=27.1461716937355, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082175, Length=444, Percent_Identity=25.4504504504505, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=412, Percent_Identity=24.5145631067961, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI86575075, Length=398, Percent_Identity=23.3668341708543, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322218, Length=388, Percent_Identity=25.7731958762887, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24642586, Length=399, Percent_Identity=26.8170426065163, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18859883, Length=209, Percent_Identity=31.1004784688995, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 45298; Mature: 45298

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHIKGGRLIDPANGIDAQQDLYLAAGKVVGVGRAPADFHANKTIDATGLVVCPGLIDL
CEEEECCCEEECCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCEECCCCEEECCCHHHH
SARLREPGYEYKATLESEMAAAMAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNLNQAH
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGLVLTEMAELTEAGCVGFSQADAPMTDTLVLMRALQYAQTFGFTVWLR
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEC
PEDPFLGKGGVAASGPLASRLGLAGVPVMAETVRLHTIFELIRSTGARVHLCRLSSAAGL
CCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
ELVRRAKAEGLPVTCDVNVHHISLTDVDIGYFNSQMRFMPPLRAGRDRDGIVRALADGTI
HHHHHHHHCCCCEEEEEEEEEEEEEEEECCEECCCCEECCCHHCCCCCHHHHHHHHCCCH
DAICSDHTPVDDDEKLLPFAEASPGATGLETLLPLTLRWAAEHKVDLPRALARITSEPAR
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEHHCCCCCHHHHHHHHCCCCHH
VLGLQAGSLEEGAMADVCVFDPDATWKVEPRSLRSQGKNSPYLGFELAGRVRATLVAGHL
EEECCCCCCCCCCCEEEEEECCCCCEEECHHHHHHCCCCCCEEEHEECCCCEEEEEEEEE
AYDGH
ECCCC
>Mature Secondary Structure
MKLHIKGGRLIDPANGIDAQQDLYLAAGKVVGVGRAPADFHANKTIDATGLVVCPGLIDL
CEEEECCCEEECCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCEECCCCEEECCCHHHH
SARLREPGYEYKATLESEMAAAMAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNLNQAH
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGLVLTEMAELTEAGCVGFSQADAPMTDTLVLMRALQYAQTFGFTVWLR
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEC
PEDPFLGKGGVAASGPLASRLGLAGVPVMAETVRLHTIFELIRSTGARVHLCRLSSAAGL
CCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
ELVRRAKAEGLPVTCDVNVHHISLTDVDIGYFNSQMRFMPPLRAGRDRDGIVRALADGTI
HHHHHHHHCCCCEEEEEEEEEEEEEEEECCEECCCCEECCCHHCCCCCHHHHHHHHCCCH
DAICSDHTPVDDDEKLLPFAEASPGATGLETLLPLTLRWAAEHKVDLPRALARITSEPAR
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEHHCCCCCHHHHHHHHCCCCHH
VLGLQAGSLEEGAMADVCVFDPDATWKVEPRSLRSQGKNSPYLGFELAGRVRATLVAGHL
EEECCCCCCCCCCCEEEEEECCCCCEEECHHHHHHCCCCCCEEEHEECCCCEEEEEEEEE
AYDGH
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]