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The map label for this gene is pyrB [H]

Identifier: 17545397

GI number: 17545397

Start: 726017

End: 726988

Strand: Direct

Name: pyrB [H]

Synonym: RSc0678

Alternate gene names: 17545397

Gene position: 726017-726988 (Clockwise)

Preceding gene: 17545396

Following gene: 17545398

Centisome position: 19.54

GC content: 64.61

Gene sequence:

>972_bases
ATGACCAAGACTTTCGCCAACCCGCAGCTCACAAAGAACGGCGAGCTCAAGCACCTGCTGTCGATCGAGGGGCTCTCGCG
TGACATCCTCACGCACATCCTCGATACGGCCAAGCAGTTCGTCTCCGTGTCGGATGCCGACCGCGAGGTGAAGAAGGTGC
CGCTGCTGCGCGGCAAGAGCGTGTTCAACCTGTTCTTCGAGAACTCCACGCGCACGCGCACCACGTTCGAGATCGCGGCC
AAGCGGCTGTCGGCGGATGTGATCAACCTGAACATCAACGCCTCCTCCACCAGCAAGGGCGAGTCGCTGCTCGATACCAT
CAACAACCTGTCGGCAATGCAGGCCGACATGTTTGTCGTGCGCCACGCCAGCTCGGGCGCGCCGTACCTGATCGCCGAGC
ACGTCGCGCCGCATGTGCACGTGATCAACGCGGGCGACGGCCGTCACGCGCACCCCACGCAGGGGCTGCTCGACATGTTC
ACCATCCGCCACTACAAGAAGGATTTCTCCAACCTGACGGTGGCGATCGTCGGCGACATCCTGCACTCGCGGGTGGCGCG
CTCCGACATCCATGCGCTGACCACGCTGGGCTGCGCCGAGGTGCGCGCCATCGGCCCGCGCACGCTGCTGCCCAGCGGTC
TGGAGCACATGGGCGTGCGCGTCTTCCACAGCATGGAAGAGGGGCTCAAGGGCGTTGACGCGGTCATCATGCTGCGCCTG
CAGAACGAACGGATGAGCGGCGCGCTGCTGCCGTCCGCGCAGGAGTATTTCAAGGCGTATGGCCTCACGCAGGAGCGGCT
GGCGCTGGCCAAGCCGGATGCCATCGTGATGCACCCGGGCCCGATGAACCGTGGCGTGGAGATCGATTCCGCCGTGGCCG
ACGGCGTGCAATCGGTGATCTTGAACCAGGTGACGTTCGGCATTGCCGTGCGGATGGCGGTGATGGGCATCGTGGCGGGC
AACAACGACTAA

Upstream 100 bases:

>100_bases
ACGCGCGAGCCCAAGACCGCCTGATGCCGGGCATCGCGTACCGCTTCTGCTCTGACCGCTTTCGTTTGTCTTCGCCTTCC
GTCCGCCTTTGTCCCCTGTC

Downstream 100 bases:

>100_bases
GAAGACCGATGAAACTGCATATCAAGGGCGGCCGCCTGATCGACCCGGCCAACGGCATCGACGCGCAACAGGATCTGTAC
CTCGCCGCGGGCAAGGTGGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKTFANPQLTKNGELKHLLSIEGLSRDILTHILDTAKQFVSVSDADREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVINLNINASSTSKGESLLDTINNLSAMQADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMF
TIRHYKKDFSNLTVAIVGDILHSRVARSDIHALTTLGCAEVRAIGPRTLLPSGLEHMGVRVFHSMEEGLKGVDAVIMLRL
QNERMSGALLPSAQEYFKAYGLTQERLALAKPDAIVMHPGPMNRGVEIDSAVADGVQSVILNQVTFGIAVRMAVMGIVAG
NND

Sequences:

>Translated_323_residues
MTKTFANPQLTKNGELKHLLSIEGLSRDILTHILDTAKQFVSVSDADREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVINLNINASSTSKGESLLDTINNLSAMQADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMF
TIRHYKKDFSNLTVAIVGDILHSRVARSDIHALTTLGCAEVRAIGPRTLLPSGLEHMGVRVFHSMEEGLKGVDAVIMLRL
QNERMSGALLPSAQEYFKAYGLTQERLALAKPDAIVMHPGPMNRGVEIDSAVADGVQSVILNQVTFGIAVRMAVMGIVAG
NND
>Mature_322_residues
TKTFANPQLTKNGELKHLLSIEGLSRDILTHILDTAKQFVSVSDADREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAK
RLSADVINLNINASSTSKGESLLDTINNLSAMQADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMFT
IRHYKKDFSNLTVAIVGDILHSRVARSDIHALTTLGCAEVRAIGPRTLLPSGLEHMGVRVFHSMEEGLKGVDAVIMLRLQ
NERMSGALLPSAQEYFKAYGLTQERLALAKPDAIVMHPGPMNRGVEIDSAVADGVQSVILNQVTFGIAVRMAVMGIVAGN
ND

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=34.3949044585987, Blast_Score=158, Evalue=5e-39,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=35.8974358974359, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786469, Length=199, Percent_Identity=28.1407035175879, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=341, Percent_Identity=31.3782991202346, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=318, Percent_Identity=32.3899371069182, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=33.7539432176656, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35134; Mature: 35003

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTFANPQLTKNGELKHLLSIEGLSRDILTHILDTAKQFVSVSDADREVKKVPLLRGKS
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMQADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMFTIRHYKKDFSNLTVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHSRVARSDIHALTTLGCAEVRAIGPRTLLPSGLEHMGVRVFHSMEEGLKGVDAVIMLRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
QNERMSGALLPSAQEYFKAYGLTQERLALAKPDAIVMHPGPMNRGVEIDSAVADGVQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LNQVTFGIAVRMAVMGIVAGNND
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKTFANPQLTKNGELKHLLSIEGLSRDILTHILDTAKQFVSVSDADREVKKVPLLRGKS
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMQADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMFTIRHYKKDFSNLTVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHSRVARSDIHALTTLGCAEVRAIGPRTLLPSGLEHMGVRVFHSMEEGLKGVDAVIMLRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
QNERMSGALLPSAQEYFKAYGLTQERLALAKPDAIVMHPGPMNRGVEIDSAVADGVQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LNQVTFGIAVRMAVMGIVAGNND
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA