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The map label for this gene is gatA [H]

Identifier: 17545308

GI number: 17545308

Start: 634916

End: 636262

Strand: Reverse

Name: gatA [H]

Synonym: RSc0589

Alternate gene names: 17545308

Gene position: 636262-634916 (Counterclockwise)

Preceding gene: 17545310

Following gene: 17545303

Centisome position: 17.12

GC content: 75.72

Gene sequence:

>1347_bases
ATGATGACACCCCGCCCGAACATGATCCGCGCCCTGGCCGCCGAGCTGGCCGCCGGCCACACCACCAGCATCGCGCTGAC
CGAAGCCGCGCTGGCGCGTGCGCAGTCGCACCGCGCGGCCGGCGGCGCGGCCTACATCGATATCGATGCGCGGGCGGCGC
TGGACATGGCGCGCGCCGCCGACGCCGCGCGCGCCGCGGGCAACGTGCCGTCGCTGCTGGCGGGACTGCCGGTGTCGATC
AAGGATCTGTTCGACGTAGCGGGCCAGGTGACGGCCGCCGGCTCGCGCGCGCTGGCGCACCAGAGCGCCGCGACCTCGGA
CGCAACCGCCGTAGCACGGCTGCGCGCGGCCGGCGCGGTGCTGCTCGGCCGCACCAACATGAGCGAATTCGCGTTCTCGG
GCCTGGGCCTGAACCCGCACTACGGCACGCCGCGCACGCCGGCGGACGGCACGCGCGCCGCAGGCGGCTCGACCTCCGGC
GGCGCGGTGACGGTGGCCGGCGGCATGGCCGTCGCCGCGCTGGGCACCGACACCGGCGGCTCGATCCGCATTCCGGCCGC
CTTCTGCGCGCTGACCGGCTTCAAGCCCACCGCGCGCCGCGTGCCGATGGCCGGCGGCGTGCCGCTGTCCACCTCGCTCG
ATTCAGGTGGGCCGCTGGCCAACTCGGTGGACTGCTGCGCCATCGTCGATGCCGTGCTCAGCGGCCAGGCGCTCGACACC
GACGCCGTGCCGCTGGCCGGCCTGCGCCTGGGACTCACGCGCGACTACGTGGCCGCGGACCTGGACGACACCGTCGCCAC
CGCGTTCGCGCGCGCCGTGGCACAGCTGGAGCGGGCGGGCGCGCACATCGTCCGCTTCGAGTTTCCCGAGCTGCTGCAGC
TGCCCGAGATCAACGGCGGCGGCGGCCTGCCCGCGGCCGAGGCCTGGGCCTGGCACCGCCCGCACCTGGCGCGCGCCGAA
GCCCAATACGACCGCCGCGTCGCCGCGCGCATCCGCCGCGGCGAGCAGATGAGCGCCGCCGCCTACCTCGACGTGATGGC
CGCGCGCGCGCGCATGATCGCGGCCGCGCGCAAGCGGCTGGGCAACCTCGATGCCTGGCTGATGCCGACCGTCGCCGTCG
TGCCGCCCGAAGTGGCGCCGCTGGAGGCCGACGACGCGCGGTTCTTCCACACCAACGCGCGGGTGCTGCGCAACCCGAGC
GCGATCAACTTCCTCGACGGCTGCGCGCTGACGCTGCCGATCCACGCCGCCGGCGAGCTGCCGGTCGGCCTGTCGCTGTG
CGGGCTGGCCGACGACGATGCGCGCATCCTGCGCGTCGGGCGGGCGGTGGAGACCGCGCTGCGCTAG

Upstream 100 bases:

>100_bases
CGAAGCCGACCAGCGACTGCGAGCGTGCCGCGACGCCGCTATGCTGTCGGCACGCCGACCGTCGCAGGCCGACGGTCACC
CACGCACCGGAGACGCCCGC

Downstream 100 bases:

>100_bases
CGCGCCGGGCAGCCTGGACCGTCGCCTTGTAGCCCATGCGCAGGCCGCCCCAATGGCGGCCGCCGACAAGGCGCGGCACG
GACACGTCCTGCATCATCGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MMTPRPNMIRALAAELAAGHTTSIALTEAALARAQSHRAAGGAAYIDIDARAALDMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALAHQSAATSDATAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGTRAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPAAFCALTGFKPTARRVPMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDT
DAVPLAGLRLGLTRDYVAADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGGGGLPAAEAWAWHRPHLARAE
AQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRLGNLDAWLMPTVAVVPPEVAPLEADDARFFHTNARVLRNPS
AINFLDGCALTLPIHAAGELPVGLSLCGLADDDARILRVGRAVETALR

Sequences:

>Translated_448_residues
MMTPRPNMIRALAAELAAGHTTSIALTEAALARAQSHRAAGGAAYIDIDARAALDMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALAHQSAATSDATAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGTRAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPAAFCALTGFKPTARRVPMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDT
DAVPLAGLRLGLTRDYVAADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGGGGLPAAEAWAWHRPHLARAE
AQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRLGNLDAWLMPTVAVVPPEVAPLEADDARFFHTNARVLRNPS
AINFLDGCALTLPIHAAGELPVGLSLCGLADDDARILRVGRAVETALR
>Mature_448_residues
MMTPRPNMIRALAAELAAGHTTSIALTEAALARAQSHRAAGGAAYIDIDARAALDMARAADAARAAGNVPSLLAGLPVSI
KDLFDVAGQVTAAGSRALAHQSAATSDATAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPRTPADGTRAAGGSTSG
GAVTVAGGMAVAALGTDTGGSIRIPAAFCALTGFKPTARRVPMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDT
DAVPLAGLRLGLTRDYVAADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGGGGLPAAEAWAWHRPHLARAE
AQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRLGNLDAWLMPTVAVVPPEVAPLEADDARFFHTNARVLRNPS
AINFLDGCALTLPIHAAGELPVGLSLCGLADDDARILRVGRAVETALR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=24.3512974051896, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI195972892, Length=233, Percent_Identity=30.4721030042918, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI166795287, Length=134, Percent_Identity=37.3134328358209, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=165, Percent_Identity=36.969696969697, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=223, Percent_Identity=31.8385650224215, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=238, Percent_Identity=34.0336134453782, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=287, Percent_Identity=27.1777003484321, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=273, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=151, Percent_Identity=37.7483443708609, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=151, Percent_Identity=37.7483443708609, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=212, Percent_Identity=30.188679245283, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=27.3417721518987, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=155, Percent_Identity=37.4193548387097, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=217, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648113, Length=303, Percent_Identity=31.6831683168317, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550774, Length=165, Percent_Identity=34.5454545454545, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648435, Length=165, Percent_Identity=34.5454545454545, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648437, Length=165, Percent_Identity=34.5454545454545, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=23.7424547283702, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356731, Length=203, Percent_Identity=33.4975369458128, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648441, Length=138, Percent_Identity=34.7826086956522, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648439, Length=138, Percent_Identity=34.7826086956522, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24644968, Length=311, Percent_Identity=26.6881028938907, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=205, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922090, Length=205, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652981, Length=205, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652983, Length=205, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 45900; Mature: 45900

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTPRPNMIRALAAELAAGHTTSIALTEAALARAQSHRAAGGAAYIDIDARAALDMARAA
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
DAARAAGNVPSLLAGLPVSIKDLFDVAGQVTAAGSRALAHQSAATSDATAVARLRAAGAV
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCEE
LLGRTNMSEFAFSGLGLNPHYGTPRTPADGTRAAGGSTSGGAVTVAGGMAVAALGTDTGG
EEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCC
SIRIPAAFCALTGFKPTARRVPMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDT
CEECCHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DAVPLAGLRLGLTRDYVAADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGG
CCCCCCCEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCC
GGLPAAEAWAWHRPHLARAEAQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRL
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GNLDAWLMPTVAVVPPEVAPLEADDARFFHTNARVLRNPSAINFLDGCALTLPIHAAGEL
CCCCHHHHCEEEECCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHCCCEEEEEEECCCCC
PVGLSLCGLADDDARILRVGRAVETALR
CCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTPRPNMIRALAAELAAGHTTSIALTEAALARAQSHRAAGGAAYIDIDARAALDMARAA
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
DAARAAGNVPSLLAGLPVSIKDLFDVAGQVTAAGSRALAHQSAATSDATAVARLRAAGAV
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCEE
LLGRTNMSEFAFSGLGLNPHYGTPRTPADGTRAAGGSTSGGAVTVAGGMAVAALGTDTGG
EEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCC
SIRIPAAFCALTGFKPTARRVPMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDT
CEECCHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DAVPLAGLRLGLTRDYVAADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGG
CCCCCCCEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCC
GGLPAAEAWAWHRPHLARAEAQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRL
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GNLDAWLMPTVAVVPPEVAPLEADDARFFHTNARVLRNPSAINFLDGCALTLPIHAAGEL
CCCCHHHHCEEEECCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHCCCEEEEEEECCCCC
PVGLSLCGLADDDARILRVGRAVETALR
CCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA