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The map label for this gene is dacB [H]

Identifier: 17545303

GI number: 17545303

Start: 627215

End: 628759

Strand: Reverse

Name: dacB [H]

Synonym: RSc0584

Alternate gene names: 17545303

Gene position: 628759-627215 (Counterclockwise)

Preceding gene: 17545308

Following gene: 17545302

Centisome position: 16.92

GC content: 70.87

Gene sequence:

>1545_bases
ATGGCCCGAATCATCCGCCTGACCCGTTTGCTCCGCCTGTCGCGCTGTGCCGTTGCCTGCGCGGCCCTCCTCGCCTTCAC
CGCCGCACCGCTGGCGCAGGCCAGAAAACCCGCCCGCGCCCATGCCGCTCCCGCCGCCACCGTCGCCCAGAAGCGGCAGG
CCGCGCACAATCCGGCCGGGCTGCCCGCCAATGTGGCGCTGGCGTTCGCGCGGGCGCATATCCCGCTCGATGCGGTCAGC
GTGTTCGTCATCCGCACCGGCACCGCCACGCCCATCCTGCAATGGAACGCCGACGCCGGCATGAATCCGGCCTCGACCAT
GAAGCTGCTGACCACCTTCGCCGGCCTCGACCTGCTGGGCCCCGATTTCCGCTGGAAGACCTCCGCCTACGCCGACAGGC
CTCCGGCGGGCGGCGTGCTCAGCGGCAACCTCTACCTGCGCGGCCAGGGCGATCCGAAGCTGATTCCCGAAGAGCTGATC
AAGCTGGTCACCGACGTGCGCCGCGCCGGCGTGGACGAGCTGGCCGGCAACATCGTGCTGGACCGCTCCTATTTCGAGAA
CGGCCTGTCCGACGCGCCGCCGCTGGACGGCGACACCGGCCGCGCCTACAACGTCGCGCCCGATGCGCTGCTCTACGCGT
TCAAGACGCTGACCTTCACGCTCGCGCCCGACGCCGCCACCGGCGCGGTCAACGTCGACGTGTCGCCGCCGCTGGCCCAG
CTGCAGGTCGACAACCAACTGCACGTGACGCACGGCAACTGCGGCGACTGGCGCTCGCATGCCAATTTCGACATCGCCAC
GCAGGCCGACGGCACGGTGCGCGCCAGCTTCGACGGGCGCTACGCCGGCGCCTGCGGCGAGCGCATCTTCAATGTCGCCG
CGCTCACCCACGCCGACTTCATCTGGGGCGGCTTCCTGGCGCTGTGGCGGCAGGCAGGCGGCACCTTCCGCGCCACGCCG
GGCCTGCGCGAGGGCAAGGTGCCGCATCCGGCCGTCCTGCTGGCGACGCACTACGGCCCGACCCTCGCCGAGGTCGTGCA
CGACATCGACAAGTATTCGAACAACGTGATGGCGCGCCAGCTGTTCCTGACCATCGGCGCCGAGATCGGCCGCAAGCCCG
CGTCGGTGCAGCAATCGGCGGACATCATCAGCCGCTGGCTCGCCCGGCAGAACCTGTCCATGCCGGAGCTGGTGCTGGAG
AACGGCTCGGGCCTCTCGCGCATCGAGCGCATCAGCGCCCGCAACCTGGGCCGCCTGCTGCAGCAGGCCGATGCCAACCC
CAACGGCGCCATCCTGCGCGACGCGCTGCCGGTGGTGGGCGTGGACGGCACCATGCGCAACCGCCTCACGCGCGCGGGCG
TGGCCGGCAACGCCGAGATCAAGACCGGCACGCTCAACGACGTGCGCGCCATCGCCGGCTACGTGGAAGGCGAAGGCGGC
CAGCGCTACGTGGTGGTCAGCATGATCAACCACCCCAGCGCCGGCGGCGGCCAGGCCGCACACGACGCCCTGCTGCAATG
GATCTACCAGGGGGCGCCGCAATGA

Upstream 100 bases:

>100_bases
CGCAGCTTGTCGGCTGGCGCCGGGGATGGCTGCGTTAACATGCGGGCGTCCGCACTCGGCGCACGCCTGTGCCTTCCCTC
CTCGTGCCCCATCCCCGTTC

Downstream 100 bases:

>100_bases
CCGCCGTCGTCGGCGTCACCGGTACCTCCGGCAGCGGCAAGACCACCCTGATCGAGCAGCTGATCGCGTGCTTCGTGCGC
GACGGCCGGCGCGTGGCCGC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MARIIRLTRLLRLSRCAVACAALLAFTAAPLAQARKPARAHAAPAATVAQKRQAAHNPAGLPANVALAFARAHIPLDAVS
VFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTSAYADRPPAGGVLSGNLYLRGQGDPKLIPEELI
KLVTDVRRAGVDELAGNIVLDRSYFENGLSDAPPLDGDTGRAYNVAPDALLYAFKTLTFTLAPDAATGAVNVDVSPPLAQ
LQVDNQLHVTHGNCGDWRSHANFDIATQADGTVRASFDGRYAGACGERIFNVAALTHADFIWGGFLALWRQAGGTFRATP
GLREGKVPHPAVLLATHYGPTLAEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVQQSADIISRWLARQNLSMPELVLE
NGSGLSRIERISARNLGRLLQQADANPNGAILRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNDVRAIAGYVEGEGG
QRYVVVSMINHPSAGGGQAAHDALLQWIYQGAPQ

Sequences:

>Translated_514_residues
MARIIRLTRLLRLSRCAVACAALLAFTAAPLAQARKPARAHAAPAATVAQKRQAAHNPAGLPANVALAFARAHIPLDAVS
VFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTSAYADRPPAGGVLSGNLYLRGQGDPKLIPEELI
KLVTDVRRAGVDELAGNIVLDRSYFENGLSDAPPLDGDTGRAYNVAPDALLYAFKTLTFTLAPDAATGAVNVDVSPPLAQ
LQVDNQLHVTHGNCGDWRSHANFDIATQADGTVRASFDGRYAGACGERIFNVAALTHADFIWGGFLALWRQAGGTFRATP
GLREGKVPHPAVLLATHYGPTLAEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVQQSADIISRWLARQNLSMPELVLE
NGSGLSRIERISARNLGRLLQQADANPNGAILRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNDVRAIAGYVEGEGG
QRYVVVSMINHPSAGGGQAAHDALLQWIYQGAPQ
>Mature_513_residues
ARIIRLTRLLRLSRCAVACAALLAFTAAPLAQARKPARAHAAPAATVAQKRQAAHNPAGLPANVALAFARAHIPLDAVSV
FVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTSAYADRPPAGGVLSGNLYLRGQGDPKLIPEELIK
LVTDVRRAGVDELAGNIVLDRSYFENGLSDAPPLDGDTGRAYNVAPDALLYAFKTLTFTLAPDAATGAVNVDVSPPLAQL
QVDNQLHVTHGNCGDWRSHANFDIATQADGTVRASFDGRYAGACGERIFNVAALTHADFIWGGFLALWRQAGGTFRATPG
LREGKVPHPAVLLATHYGPTLAEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVQQSADIISRWLARQNLSMPELVLEN
GSGLSRIERISARNLGRLLQQADANPNGAILRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNDVRAIAGYVEGEGGQ
RYVVVSMINHPSAGGGQAAHDALLQWIYQGAPQ

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=433, Percent_Identity=26.0969976905312, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 54616; Mature: 54485

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIIRLTRLLRLSRCAVACAALLAFTAAPLAQARKPARAHAAPAATVAQKRQAAHNPAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCC
LPANVALAFARAHIPLDAVSVFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLG
CCHHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCC
PDFRWKTSAYADRPPAGGVLSGNLYLRGQGDPKLIPEELIKLVTDVRRAGVDELAGNIVL
CCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCEEE
DRSYFENGLSDAPPLDGDTGRAYNVAPDALLYAFKTLTFTLAPDAATGAVNVDVSPPLAQ
EHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHEEEECCCCCCCEEEECCCCCHHH
LQVDNQLHVTHGNCGDWRSHANFDIATQADGTVRASFDGRYAGACGERIFNVAALTHADF
EEECCEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IWGGFLALWRQAGGTFRATPGLREGKVPHPAVLLATHYGPTLAEVVHDIDKYSNNVMARQ
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHH
LFLTIGAEIGRKPASVQQSADIISRWLARQNLSMPELVLENGSGLSRIERISARNLGRLL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHH
QQADANPNGAILRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNDVRAIAGYVEGEGG
HHHCCCCCCCCHHCCCCEEECCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHEECCCC
QRYVVVSMINHPSAGGGQAAHDALLQWIYQGAPQ
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARIIRLTRLLRLSRCAVACAALLAFTAAPLAQARKPARAHAAPAATVAQKRQAAHNPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCC
LPANVALAFARAHIPLDAVSVFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLG
CCHHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCC
PDFRWKTSAYADRPPAGGVLSGNLYLRGQGDPKLIPEELIKLVTDVRRAGVDELAGNIVL
CCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCEEE
DRSYFENGLSDAPPLDGDTGRAYNVAPDALLYAFKTLTFTLAPDAATGAVNVDVSPPLAQ
EHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHEEEECCCCCCCEEEECCCCCHHH
LQVDNQLHVTHGNCGDWRSHANFDIATQADGTVRASFDGRYAGACGERIFNVAALTHADF
EEECCEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IWGGFLALWRQAGGTFRATPGLREGKVPHPAVLLATHYGPTLAEVVHDIDKYSNNVMARQ
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHH
LFLTIGAEIGRKPASVQQSADIISRWLARQNLSMPELVLENGSGLSRIERISARNLGRLL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHH
QQADANPNGAILRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNDVRAIAGYVEGEGG
HHHCCCCCCCCHHCCCCEEECCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHEECCCC
QRYVVVSMINHPSAGGGQAAHDALLQWIYQGAPQ
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]