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The map label for this gene is pykA1 [H]

Identifier: 17545291

GI number: 17545291

Start: 615703

End: 617142

Strand: Direct

Name: pykA1 [H]

Synonym: RSc0572

Alternate gene names: 17545291

Gene position: 615703-617142 (Clockwise)

Preceding gene: 17545290

Following gene: 17545292

Centisome position: 16.57

GC content: 68.89

Gene sequence:

>1440_bases
ATGACTCGCGCCACCAAGATCGTCGCCACACTCGGCCCTTCGTCCAATACGCTGGAGATCCTGACGCGCATGATCGCGGC
GGGGGTGGACGTGGTGCGGCTGAACTTCTCGCACGGCACCGCGCAGGACCACGTGGCGCGCGCCGAGCTGGTGCGCGAGA
CGGCGCGCGCGGTGGGCCGCGAGGTCGGCATCATGGCCGACCTGCAGGGCCCGAAGATCCGCGTCGGCAAGTTCGAGAGC
GGCAAGATCACCCTGAGCCCGGGCGAGCGCTTCACGCTGGATGCCCGCTGCGAGCTGGGCAACCAGGAGCGCGTCGGCCT
GGACTACAAGGAGCTGCCGCGCGACGTCGGCCCCGGTGACCTGCTGCTGCTCAACGACGGCCTGATCGTGCTGCAGGTCG
AGCGCGTGGTGGGCGAGGAGATCGAGACCGTCGTCAAGGTCGGCGGCGATCTGTCCAACAACAAGGGCATCAACCGCCAG
GGCGGCGGGCTGTCGGCGCCGGCGCTGACGGCCAAGGACATGGAAGACATCAAGACCGCGATGGCGCTGGGCGCCGACTA
CATCGCGGTCAGCTTCCCCAAGAACGCCACCGACATGGAAATGGCGCGCCAGCTGGCCGCCGTGGCCGGCGCGCCGCACA
GCCACAAGACCAAGATGATCGCCAAGATCGAGCGTGCCGAGGCCATCCGCCCGGGCGTGCTGGAAGAGATCCTGGCGGCG
TCCGACGGCATCATGGTGGCGCGCGGCGACCTCGCGGTGGAGGTCGGCAACGCGGCCGTGCCGGCGCTGCAGAAGCGCAT
GATCAAGCTCGCGCGCGAGGCCAACAAGCTGGCCATCACGGCCACGCAGATGATGGAGTCGATGATCGTCAACCCGGTGC
CGACGCGCGCCGAGGTGTCGGACGTCGCCAACGCCGTGCTCGACGGCACCGATGCGGTGATGCTGTCGGCCGAGACCGCC
GCCGGCCGCTACCCGGTCGAGACCATCGAGGCCATGGCCGCCATCTGCGTGGAGGCCGAGAAGTCGCAGCCGGTGCACCT
CGACACGGATTTCCTCGACCAGACCTTCAGCCGCATCGACCAGTCGATCGCCATGGGGGCGCTGTTTACGGCCTACCATC
TGCAGGTCAAGGCGATTGCCGCGCTGACCGACTCAGGTGCCACCGCGCTGTGGATGAGCCGCCACGGCATCAATGTGCCG
ATCTATGCGATCACGCCCAACGTCGCGTCGCAGCGCAAGATGGCCCTGTACCGCAACGTGCAGCCGCTGCCGCTGGCCAT
CAGCCTGGACCGCGACGAGGCGCTGCGGCAGGCCGAGGAGCTGCTGGTCGCGCGCGGCGTGGTGCAGCGCGGCGACTTCA
TCGTCCTGACCGTCGGCGAGCCGATGGGGCAGGCGGGCGGGACCAACACACTCAAGATCGTGCGCGTCGGCATGCACTGA

Upstream 100 bases:

>100_bases
CTGGAGCAGCGTGCCCAGCGCCAGGAAGCGCTGGCCTGAAGCGCCAGGCACGTCGCCTGCGTCGTCAACGTCCCCCAAAC
GTCACCGGTACCCCTTGCCC

Downstream 100 bases:

>100_bases
CGCGCCACCGGGAATGCCGGTTGCTACCGGCATCCGTGAGACCGAATACAGATCGACCCATAGGAGAAAACCATGCCACT
CGTCTCGATGCGCCAACTGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTRATKIVATLGPSSNTLEILTRMIAAGVDVVRLNFSHGTAQDHVARAELVRETARAVGREVGIMADLQGPKIRVGKFES
GKITLSPGERFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETVVKVGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYIAVSFPKNATDMEMARQLAAVAGAPHSHKTKMIAKIERAEAIRPGVLEEILAA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETIEAMAAICVEAEKSQPVHLDTDFLDQTFSRIDQSIAMGALFTAYHLQVKAIAALTDSGATALWMSRHGINVP
IYAITPNVASQRKMALYRNVQPLPLAISLDRDEALRQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH

Sequences:

>Translated_479_residues
MTRATKIVATLGPSSNTLEILTRMIAAGVDVVRLNFSHGTAQDHVARAELVRETARAVGREVGIMADLQGPKIRVGKFES
GKITLSPGERFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETVVKVGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYIAVSFPKNATDMEMARQLAAVAGAPHSHKTKMIAKIERAEAIRPGVLEEILAA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETIEAMAAICVEAEKSQPVHLDTDFLDQTFSRIDQSIAMGALFTAYHLQVKAIAALTDSGATALWMSRHGINVP
IYAITPNVASQRKMALYRNVQPLPLAISLDRDEALRQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
>Mature_478_residues
TRATKIVATLGPSSNTLEILTRMIAAGVDVVRLNFSHGTAQDHVARAELVRETARAVGREVGIMADLQGPKIRVGKFESG
KITLSPGERFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETVVKVGGDLSNNKGINRQG
GGLSAPALTAKDMEDIKTAMALGADYIAVSFPKNATDMEMARQLAAVAGAPHSHKTKMIAKIERAEAIRPGVLEEILAAS
DGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETAA
GRYPVETIEAMAAICVEAEKSQPVHLDTDFLDQTFSRIDQSIAMGALFTAYHLQVKAIAALTDSGATALWMSRHGINVPI
YAITPNVASQRKMALYRNVQPLPLAISLDRDEALRQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=496, Percent_Identity=37.5, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286420, Length=496, Percent_Identity=37.5, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286418, Length=499, Percent_Identity=37.4749498997996, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI10835121, Length=502, Percent_Identity=36.4541832669323, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI32967597, Length=502, Percent_Identity=36.4541832669323, Blast_Score=265, Evalue=9e-71,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=36.6438356164384, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=36.6438356164384, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=33.75, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=33.75, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128738, Length=219, Percent_Identity=32.8767123287671, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.4644351464435, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=37.6321353065539, Blast_Score=298, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.9609856262834, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=37.8818737270876, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=37.6782077393075, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=38.2892057026477, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=37.6782077393075, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=34.0770791075051, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=35.1020408163265, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=36.8421052631579, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=36.8421052631579, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=36.9565217391304, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=31.4285714285714, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24646914, Length=184, Percent_Identity=44.0217391304348, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51134; Mature: 51003

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRATKIVATLGPSSNTLEILTRMIAAGVDVVRLNFSHGTAQDHVARAELVRETARAVGR
CCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EVGIMADLQGPKIRVGKFESGKITLSPGERFTLDARCELGNQERVGLDYKELPRDVGPGD
HCCEEEECCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLQVERVVGEEIETVVKVGGDLSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYIAVSFPKNATDMEMARQLAAVAGAPHSHKTKMIAKIERAEAIRPGVLEEILAA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLAITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETIEAMAAICVEAEKSQPVHLDTDFLDQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHH
QSIAMGALFTAYHLQVKAIAALTDSGATALWMSRHGINVPIYAITPNVASQRKMALYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCC
QPLPLAISLDRDEALRQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TRATKIVATLGPSSNTLEILTRMIAAGVDVVRLNFSHGTAQDHVARAELVRETARAVGR
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EVGIMADLQGPKIRVGKFESGKITLSPGERFTLDARCELGNQERVGLDYKELPRDVGPGD
HCCEEEECCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLQVERVVGEEIETVVKVGGDLSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYIAVSFPKNATDMEMARQLAAVAGAPHSHKTKMIAKIERAEAIRPGVLEEILAA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLAITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETIEAMAAICVEAEKSQPVHLDTDFLDQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHH
QSIAMGALFTAYHLQVKAIAALTDSGATALWMSRHGINVPIYAITPNVASQRKMALYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCC
QPLPLAISLDRDEALRQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]