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The map label for this gene is rsmD [H]

Identifier: 17545108

GI number: 17545108

Start: 418053

End: 418700

Strand: Direct

Name: rsmD [H]

Synonym: RSc0389

Alternate gene names: 17545108

Gene position: 418053-418700 (Clockwise)

Preceding gene: 17545107

Following gene: 17545109

Centisome position: 11.25

GC content: 71.6

Gene sequence:

>648_bases
ATGGCTTCCCGTACTCCCAATCGTCCCAAGACTTCATCCGCCACGTCGCATGCCCGCGCGCCGCACCAGGTGCGGATCAT
CGGCGGGCAGTACAAGCGCACGCCGCTGCCGGTGATCGATGCCGAGGGCCTGCGCCCGACCGGCGATCGCGTTCGCGAGA
CCCTGTTCAACTGGCTGGGCCAGGACCTGGCCGGCTGGCGCTGCGCCGATCTCTTCGCCGGTACCGGCGCGCTCGGCTTC
GAGGCCGCCTCGCGCGGCGCGGCGCGGGTCACGCTGGTGGAAAGCCATGCGCCCGCGCTGCGCGCCCTGCACGCGGTGCG
CGACAAGCTGCGCGCCGGCATGGTCGACATCGTGGCGGGTGATGTCTTCGCCTGGCTCGCGCGCCAGGCCGACGGCGCTT
TCGACCTGGTCTTCATCGATCCGCCGTTTGCGCAGGACTGGACCCTGCGGGCGCTGGAAGCCGCGCTGCGCGTGGTGCCC
GAGGGTGGGCTGATCTACGTGGAATCGCCGCGTCCGCTGGTGGCGGTCGAGCCGGGCGAAGGCGTTGCACCGGAGGCCGG
CACGCCGCTTCCGGCCGGCGTGGTGCTGCACAGGCACCTGCGCGCCGGCGCGGTGCATGCCCATTTGCTGCTGCGCAAAA
ACGGTTAA

Upstream 100 bases:

>100_bases
GCGGTCCGGCACCGCACTGACGGCCGGGTTGGCTCCCGAGCGCAAAAAACCGGCGGCATCCCCGCCGGTTTTTGTTTGCG
CAGGCTCTACAATCGCGCCC

Downstream 100 bases:

>100_bases
ACTACGCGGCTTGTCGCCGGTGCATGGGCAGCCGGCGGCACGTTGCCGCCGGGCTTGGGCGTTCCGACCTGATCCCGGCA
ATGCGGGCGGCAGATTCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MASRTPNRPKTSSATSHARAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLGQDLAGWRCADLFAGTGALGF
EAASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAGDVFAWLARQADGAFDLVFIDPPFAQDWTLRALEAALRVVP
EGGLIYVESPRPLVAVEPGEGVAPEAGTPLPAGVVLHRHLRAGAVHAHLLLRKNG

Sequences:

>Translated_215_residues
MASRTPNRPKTSSATSHARAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLGQDLAGWRCADLFAGTGALGF
EAASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAGDVFAWLARQADGAFDLVFIDPPFAQDWTLRALEAALRVVP
EGGLIYVESPRPLVAVEPGEGVAPEAGTPLPAGVVLHRHLRAGAVHAHLLLRKNG
>Mature_214_residues
ASRTPNRPKTSSATSHARAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLGQDLAGWRCADLFAGTGALGFE
AASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAGDVFAWLARQADGAFDLVFIDPPFAQDWTLRALEAALRVVPE
GGLIYVESPRPLVAVEPGEGVAPEAGTPLPAGVVLHRHLRAGAVHAHLLLRKNG

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=198, Percent_Identity=40.9090909090909, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22983; Mature: 22851

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRTPNRPKTSSATSHARAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLG
CCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
QDLAGWRCADLFAGTGALGFEAASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAG
HHHCCCCHHHHHCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
DVFAWLARQADGAFDLVFIDPPFAQDWTLRALEAALRVVPEGGLIYVESPRPLVAVEPGE
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCC
GVAPEAGTPLPAGVVLHRHLRAGAVHAHLLLRKNG
CCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure 
ASRTPNRPKTSSATSHARAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLG
CCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
QDLAGWRCADLFAGTGALGFEAASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAG
HHHCCCCHHHHHCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
DVFAWLARQADGAFDLVFIDPPFAQDWTLRALEAALRVVPEGGLIYVESPRPLVAVEPGE
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCC
GVAPEAGTPLPAGVVLHRHLRAGAVHAHLLLRKNG
CCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]