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The map label for this gene is amtB [H]

Identifier: 17545062

GI number: 17545062

Start: 374899

End: 376209

Strand: Direct

Name: amtB [H]

Synonym: RSc0343

Alternate gene names: 17545062

Gene position: 374899-376209 (Clockwise)

Preceding gene: 17545061

Following gene: 17545063

Centisome position: 10.09

GC content: 66.13

Gene sequence:

>1311_bases
GTGCCCAACAAGGGCGACACGGCGTGGCTGCTGGTTTCGACCGCCTTCGTGATCCTGATGACGCTGCCGGGCCTGGCGCT
GTTCTACGGCGGCCTGGTGCGCAAGAAGAACATGCTGTCGGTCCTGATGCAATGTACCGGCATCTTCTCGCTCATCATGA
TCCTGTGGGCCATCTACGGCTACAGCATCGCCTTCACCGAGGGCAATGCCTTCTTCGGCGGGCTCGACCGGCTGTTCCTG
AAGGGTCTGACGCCGGATTCGGTTGCCGCTACGTTCAGCAAGGGCGTGGTAGTGCCGGAGTATGCGTACTTCGCCTTCCA
GGGCGCATTTGCCGCGATCACCTGCGCGCTGATCATCGGTGCCTTCGCCGAGCGCGCCAAGTTCTCGGCCGTGCTGGTCT
TCGTGGTGCTGTGGTTCACCTTTGCCTACCTGCCGATCGCCCACATGGTCTGGTTCTGGCCGGGGCCGGACGGCTTCACC
GACGCCAAGGCGGCCGAAGTGATGACGGCCAAGGGCGGCTGGCTGTTCCAGAAGGGCGCGCTGGACTTCGCCGGCGGCAC
CGTGGTGCACATCAACGCCGCCGTGGCGGGCCTGGTGGGTGCCTTCCTGTTCGGCAAGCGGATCGGCTTCGGCCGCGAAG
CGCTCAAGCCGCATAGCCTGACGCTGACCATGGTCGGTGCCTCGCTGCTGTGGTTCGGCTGGTTCGGCTTCAACGCCGGT
TCGGCCCTGGAAGCCAACGGCAGCGCCGCGCTGGCCTTCGTCAACACGCTGCTCGCCACCGCCGCCGCCGTGTTGTCGTG
GAGCTTCGGCGAGTGGATCGGCAAGAGCAAGCCGTCAATGCTGGGTGCCGCGTCGGGCGCCGTCGCCGGCCTGGTGGCGA
TCACCCCGGCAGCCGGCTTCGTCGGCCCGATGGGCGCCATCGCGATGGGCATCCTCGCCGGCGTGCTGTGCCTGTGGGGC
GTCAACGGCCTCAAGCGCATGCTGGGCATGGACGACACGCTGGACGTGTTCGGCGTGCACGGCGTGGGCGGCATCATGGG
CGCGCTGCTGACCGGCGTGTTCGCCTCGCCGAGCCTGGGCGGCACCGGCATCTACGACTATGTCGCCAACAAGGTGGCCG
ATGGCTACTCGATCGGCGGTCAGCTGTGGATTCAGCTGCAGGGCGTGCTGACCACCATCGTGTGGTCTGCCGTGGTGGCT
TTCGTTGCCTACAAGATCGTGGACGCGATCATCGGTCTGCGCGTGCCGGAAGAAGAGGAGCGCGAAGGTCTGGACATCAC
GTCTCACGGCGAAACCGCTTACGAAGACTGA

Upstream 100 bases:

>100_bases
TGCCGCCGCGGCTTCCGAGCCGGCCGCGGCATCGGCTGCTGCTGCCGCTCCGGCCGCCGCCGCACCCGCGGCCGCATCGG
CGCCGGCGGCTGCCACGCCG

Downstream 100 bases:

>100_bases
GCGTTTTCCACAGCGTTACCGCAAAGGACCCGGCCTTGTGCCGGGTTTTTTGTCGCCGCGCTTCAACCGGGGGAGCGCGG
ACGGGCGGGCCGATCGGGGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYGYSIAFTEGNAFFGGLDRLFL
KGLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFT
DAKAAEVMTAKGGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAG
SALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGAIAMGILAGVLCLWG
VNGLKRMLGMDDTLDVFGVHGVGGIMGALLTGVFASPSLGGTGIYDYVANKVADGYSIGGQLWIQLQGVLTTIVWSAVVA
FVAYKIVDAIIGLRVPEEEEREGLDITSHGETAYED

Sequences:

>Translated_436_residues
MPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYGYSIAFTEGNAFFGGLDRLFL
KGLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFT
DAKAAEVMTAKGGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAG
SALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGAIAMGILAGVLCLWG
VNGLKRMLGMDDTLDVFGVHGVGGIMGALLTGVFASPSLGGTGIYDYVANKVADGYSIGGQLWIQLQGVLTTIVWSAVVA
FVAYKIVDAIIGLRVPEEEEREGLDITSHGETAYED
>Mature_435_residues
PNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYGYSIAFTEGNAFFGGLDRLFLK
GLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAFAERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFTD
AKAAEVMTAKGGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAGS
ALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAASGAVAGLVAITPAAGFVGPMGAIAMGILAGVLCLWGV
NGLKRMLGMDDTLDVFGVHGVGGIMGALLTGVFASPSLGGTGIYDYVANKVADGYSIGGQLWIQLQGVLTTIVWSAVVAF
VAYKIVDAIIGLRVPEEEEREGLDITSHGETAYED

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=433, Percent_Identity=55.8891454965358, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17550182, Length=458, Percent_Identity=29.4759825327511, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=450, Percent_Identity=28.6666666666667, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=448, Percent_Identity=26.7857142857143, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=24.9438202247191, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=453, Percent_Identity=36.4238410596026, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=440, Percent_Identity=32.7272727272727, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=435, Percent_Identity=32.183908045977, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI161078314, Length=441, Percent_Identity=26.7573696145125, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45750; Mature: 45619

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YSIAFTEGNAFFGGLDRLFLKGLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIG
CEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFAERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFTDAKAAEVMTAKGGWLFQKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHEECCCCEEEECCC
LDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAG
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHHHHHHHHHHHCCCCC
SALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAASGAVAGLVAITPAAGF
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCC
VGPMGAIAMGILAGVLCLWGVNGLKRMLGMDDTLDVFGVHGVGGIMGALLTGVFASPSLG
CCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCC
GTGIYDYVANKVADGYSIGGQLWIQLQGVLTTIVWSAVVAFVAYKIVDAIIGLRVPEEEE
CCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
REGLDITSHGETAYED
HCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YSIAFTEGNAFFGGLDRLFLKGLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIG
CEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFAERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFTDAKAAEVMTAKGGWLFQKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHEECCCCEEEECCC
LDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMVGASLLWFGWFGFNAG
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHHHHHHHHHHHCCCCC
SALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAASGAVAGLVAITPAAGF
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCC
VGPMGAIAMGILAGVLCLWGVNGLKRMLGMDDTLDVFGVHGVGGIMGALLTGVFASPSLG
CCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCC
GTGIYDYVANKVADGYSIGGQLWIQLQGVLTTIVWSAVVAFVAYKIVDAIIGLRVPEEEE
CCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
REGLDITSHGETAYED
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]