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The map label for this gene is 17545063

Identifier: 17545063

GI number: 17545063

Start: 376641

End: 377936

Strand: Direct

Name: 17545063

Synonym: RSc0344

Alternate gene names: NA

Gene position: 376641-377936 (Clockwise)

Preceding gene: 17545062

Following gene: 17545064

Centisome position: 10.13

GC content: 64.35

Gene sequence:

>1296_bases
ATGGTTCCGCACCTGATCACCGCGTTGAAAGGGCCGCTGTTGGAGCTCGAGCAGAAGATTCTCGATGCGACGCCCGCGAT
CGAACGCTGGTTCCGCCTGGAGTGGCAGGAACATACGCCGCCGTTCTACTGCTCGGTCGACCTGCGCAACGCCGGCTTCA
AGCTGGCGCCGGTCGACACCAACCTGTTCCCCGGCGGCTTCAACAACCTCGCGCCCGAGATGCTGCCGCTGGCTGTGCAG
GCCGCGATGGCCGCGATTGAGAAGATCTGCCCGGATGCCAAGAACCTGCTGCTGATCCCCGAGCGCCATACGCGCAACAT
GTTCTATCTGCAGAACGTGGCGCGGCTGTCGCAGATCATGCGCCAGGCCGGGCTGAATGTGCGCCTGGGCTCGCTGTCCG
AAGACGTCAAGGAACCCACGCCGATCGACCTGCCCGACGGCCAGCGCCTGGTGGTCGAGCCGATCAAGCGCATCGGCACC
AAGGACCGCCGGCTGGGCATGGACGACTTCGATCCGTGCTCGATCCTGCTGAACAACGACCTGTCCGCGGGCGTGCCGCC
CATCCTGGAGAACATCAACGAGCAATATCTGCTGCCGCCGCTCCATGCGGGCTGGTCGCAGCGGCGCAAATCGAACCACT
TTGCCGCCTACGACGAGGTGGCCAAGAAGTTCGCCAAGCTGATCGACATCGACCCGTGGATGGTCAACCCGTACTTCGCC
AAGTGCAACGGCATCGACTTCCACGAGCGCGCCGGCGAGGACGAGCTCGCCCACGCCGTCGAGACCGTGCTCAAGAAGAC
CGCCAAGAAATACAAGGAATACGGCATCCACGAGACACCCTATGCGGTGGTCAAGGCCGATGCCGGCACCTATGGCATGG
GCATCATGACCGTGCGCGATCCGTCCGAGGTCCGGGGCCTGAACCGGAAGGAGCGCAACAAGATGAGCGTGGTCAAGGAG
GGCCTCGAGGTCTCCGAGGTGATCGTGCAGGAAGGCGTGCACACCTTCGAGAAGATCAACGAGGCGGTGGCCGAGCCGGT
CGTCTACATGATCGATCGCTATGTGGTCGGCGGGTTCTACCGGGTACACACCGGCCGCGGCATCGACGAGAATCTGAACG
CGCCGGGCATGCATTTCGTGCCGCTGCCGTTCGCGTCGAATTCGCTGCCGGACAGCCACGCCAAGCCGGGCGCCGGCGAG
CCGAACCGCTTCTACATGTACGGCGTGGTGGCGCGCCTGGCGCTGCTGGCCGCGTCGCTCGAACTCGAGAAGACCGACCC
GAACCCGCTGATCTGA

Upstream 100 bases:

>100_bases
CGGCCAGCCGGCCGCATATCGATAGACCTCCGCCGTCTGCAAGCAATTGCCCATTCCTCTACAATCGGAGCGGGCGATAA
ATTACATCAATAGGAAGGGT

Downstream 100 bases:

>100_bases
TTCCTGTAATACGGTCCCTGGAGCCGCCATGCGCATCCTCTTCATCGTCGATCCGCTGTCAACATTCAAGATTTACAAGG
ATTCCACCTTCGCGATGATG

Product: hypothetical protein

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; GshA Protein; Glutathione Synthetase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALKGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDVKEPTPIDLPDGQRLVVEPIKRIGT
KDRRLGMDDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKSNHFAAYDEVAKKFAKLIDIDPWMVNPYFA
KCNGIDFHERAGEDELAHAVETVLKKTAKKYKEYGIHETPYAVVKADAGTYGMGIMTVRDPSEVRGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI

Sequences:

>Translated_431_residues
MVPHLITALKGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDVKEPTPIDLPDGQRLVVEPIKRIGT
KDRRLGMDDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKSNHFAAYDEVAKKFAKLIDIDPWMVNPYFA
KCNGIDFHERAGEDELAHAVETVLKKTAKKYKEYGIHETPYAVVKADAGTYGMGIMTVRDPSEVRGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI
>Mature_431_residues
MVPHLITALKGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIMRQAGLNVRLGSLSEDVKEPTPIDLPDGQRLVVEPIKRIGT
KDRRLGMDDFDPCSILLNNDLSAGVPPILENINEQYLLPPLHAGWSQRRKSNHFAAYDEVAKKFAKLIDIDPWMVNPYFA
KCNGIDFHERAGEDELAHAVETVLKKTAKKYKEYGIHETPYAVVKADAGTYGMGIMTVRDPSEVRGLNRKERNKMSVVKE
GLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLPFASNSLPDSHAKPGAGE
PNRFYMYGVVARLALLAASLELEKTDPNPLI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48225; Mature: 48225

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALKGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEDVKEPTPIDLPDGQRLVVEPIKRIGTKDRRLGMDDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCC
LSAGVPPILENINEQYLLPPLHAGWSQRRKSNHFAAYDEVAKKFAKLIDIDPWMVNPYFA
CCCCCCHHHHCCCCCEECCCHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
KCNGIDFHERAGEDELAHAVETVLKKTAKKYKEYGIHETPYAVVKADAGTYGMGIMTVRD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECC
PSEVRGLNRKERNKMSVVKEGLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGIDENLNAPGMHFVPLPFASNSLPDSHAKPGAGEPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELEKTDPNPLI
EEECCCCCCCC
>Mature Secondary Structure
MVPHLITALKGPLLELEQKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSQIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEDVKEPTPIDLPDGQRLVVEPIKRIGTKDRRLGMDDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCC
LSAGVPPILENINEQYLLPPLHAGWSQRRKSNHFAAYDEVAKKFAKLIDIDPWMVNPYFA
CCCCCCHHHHCCCCCEECCCHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
KCNGIDFHERAGEDELAHAVETVLKKTAKKYKEYGIHETPYAVVKADAGTYGMGIMTVRD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECC
PSEVRGLNRKERNKMSVVKEGLEVSEVIVQEGVHTFEKINEAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGIDENLNAPGMHFVPLPFASNSLPDSHAKPGAGEPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELEKTDPNPLI
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA