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The map label for this gene is dctA1

Identifier: 17545049

GI number: 17545049

Start: 361146

End: 362432

Strand: Direct

Name: dctA1

Synonym: RSc0330

Alternate gene names: 17545049

Gene position: 361146-362432 (Clockwise)

Preceding gene: 17545044

Following gene: 17545050

Centisome position: 9.72

GC content: 62.86

Gene sequence:

>1287_bases
ATGAAAAAACCGTTCTACAAAATCCTGTACGTACAGGTGCTGGCGGCCATCGTCATCGGTGTGCTGCTGGGCCATTTCGA
GCCCAAGCTGGCCGTCAACATGAAGCCGCTGGGCGATGGCTTCATTCAGCTGATCAAGATGGTGATCGGGCCGATCATCT
TCTGTACGGTCGTCTCCGGCATCGCCGGCATGCGCGACATGAAGAAGGTGGGCCGCGTGGGCGGCAAGGCGCTGCTCTAC
TTCGAGGTCGTCTCGACCTTCGCGCTGGTGATTGGTCTGGTTGCCGGCCACATCTTCAACCCGGGCTCGGGCTTCAACGT
CGACGTCAACTCGATCGACGCCAAGGCCGTGGCCCAGTACGCCGCCAAGGCGCAATCGTCGAGCACGGTCGATTTCCTGC
TGAACATCATCCCGAGCACGGTGGTGGATGCCTTTGCCAAGGGCGACATCCTGCAGATCTTGCTGATTGCGCTGCTGTTC
GGCGGTGCGCTCTCCGCAATGGGTGAGCGCGCGCAGATGGTGACGGACTTCATTGACCAGATCGCCCACGTGTTCTTCCG
CATCGTGCACGTCATCACGCGCGTGGCCCCGATCGGCGCGTTCGGCGCGATGGCCTTCACCATCGGCAAGTACGGTGTGG
TGTCGCTGGTGCCGCTGCTCAAGCTGATCGGCACGTTCTACCTGACAGCCATCATCTTCGTGGTGGTGGTGCTGGGCATC
ATCGCCCGCCTGACCGGCTTCAGCATCTTCCGCTTCGTGGCGTACATCAAGGAAGAGCTGCTGATCGTGCTGGGCACCAG
CTCGTCGGAATCGGCGCTGCCGCACCTGATGGAAAAGATGGAGAAGCTGGGCTGCTCGAAGTCGGTGGTGGGCCTCGTTG
TCCCGACCGGCTATTCGTTCAACCTGGACGGCACCAACATCTACATGACGATGGCGGTGATCTTCATCTCGCAGGCGCTG
AACATCGAACTGACCTTGACCCAGCAACTGACCATCCTGGCGGTGGCGATGCTGACCTCCAAGGGCGCCTCGGGCATCAC
CGGCGCGGGCTTCATCACGCTGGCCGCCACGCTGGCCGTGGTGCCGACCATTCCGGTGGCGGGCATGGTGCTGATCCTCG
GCATCGACCGCTTCATGAGCGAGTGCCGCGCGCTGACCAACATCACCGGCAACGGCGTGGCCTGCGTGGTGATCTCCGCC
TGGGAACGCGAGCTCGACCGCACCAAGCTGGCCCGCGTGATGTCGGGCGATCGCGGCGAGACCGTGTCGGCCACCCCGGC
AGCCTGA

Upstream 100 bases:

>100_bases
GGCCATCCCCGGCGCCACGGCGGGGAGAGAAACCACATCAGGGCGGGTCGGACCGGGCGGGCAATGAACGCGCGGCCGGT
CTGTGACACTCGAGGAAGAC

Downstream 100 bases:

>100_bases
TGGGCCAGAGCCGGCGGTGCCCCACCGGGGCGTCGCCGGATGGCAAGCGTATGATGGCGGGGCCGCTCCTTCCGGACGGC
CCCGCTTTCTTTTGGAAAGT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKPFYKILYVQVLAAIVIGVLLGHFEPKLAVNMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALVIGLVAGHIFNPGSGFNVDVNSIDAKAVAQYAAKAQSSSTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQIAHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVVSLVPLLKLIGTFYLTAIIFVVVVLGI
IARLTGFSIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTLTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRTKLARVMSGDRGETVSATPAA

Sequences:

>Translated_428_residues
MKKPFYKILYVQVLAAIVIGVLLGHFEPKLAVNMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALVIGLVAGHIFNPGSGFNVDVNSIDAKAVAQYAAKAQSSSTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQIAHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVVSLVPLLKLIGTFYLTAIIFVVVVLGI
IARLTGFSIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTLTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRTKLARVMSGDRGETVSATPAA
>Mature_428_residues
MKKPFYKILYVQVLAAIVIGVLLGHFEPKLAVNMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALVIGLVAGHIFNPGSGFNVDVNSIDAKAVAQYAAKAQSSSTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQIAHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVVSLVPLLKLIGTFYLTAIIFVVVVLGI
IARLTGFSIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTLTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRTKLARVMSGDRGETVSATPAA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=434, Percent_Identity=26.036866359447, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4827012, Length=286, Percent_Identity=30.4195804195804, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI21314632, Length=436, Percent_Identity=25.6880733944954, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI40254478, Length=429, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI5032093, Length=392, Percent_Identity=27.5510204081633, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI66773030, Length=408, Percent_Identity=23.5294117647059, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194239697, Length=455, Percent_Identity=24.6153846153846, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI223468564, Length=267, Percent_Identity=29.2134831460674, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI223468566, Length=267, Percent_Identity=29.2134831460674, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI262359914, Length=382, Percent_Identity=25.130890052356, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601644, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=61.6867469879518, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1790514, Length=413, Percent_Identity=39.9515738498789, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788024, Length=404, Percent_Identity=20.2970297029703, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789473, Length=218, Percent_Identity=26.1467889908257, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=427, Percent_Identity=26.2295081967213, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI193206505, Length=450, Percent_Identity=24.6666666666667, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=481, Percent_Identity=23.2848232848233, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983106, Length=481, Percent_Identity=23.2848232848233, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=433, Percent_Identity=23.5565819861432, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71996953, Length=466, Percent_Identity=22.7467811158798, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=430, Percent_Identity=22.3255813953488, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=428, Percent_Identity=21.9626168224299, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137668, Length=428, Percent_Identity=21.9626168224299, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583023, Length=428, Percent_Identity=21.9626168224299, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360481, Length=463, Percent_Identity=24.4060475161987, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281360483, Length=463, Percent_Identity=24.4060475161987, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137666, Length=463, Percent_Identity=24.4060475161987, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA1_RALSO (Q8Y2K5)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518451.1
- ProteinModelPortal:   Q8Y2K5
- SMR:   Q8Y2K5
- GeneID:   1219133
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0330
- NMPDR:   fig|267608.1.peg.330
- HOGENOM:   HBG739804
- OMA:   ALATDMK
- ProtClustDB:   PRK01663
- BioCyc:   RSOL267608:RSC0330-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 45433; Mature: 45433

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbca0084)-; HASH(0x13e184a8)-; HASH(0x1370a988)-; HASH(0x13e1d0b0)-; HASH(0x13b4f5ac)-; HASH(0x1378c3a8)-; HASH(0x13e1e0a0)-; HASH(0x13e19f00)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFYKILYVQVLAAIVIGVLLGHFEPKLAVNMKPLGDGFIQLIKMVIGPIIFCTVVSG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
IAGMRDMKKVGRVGGKALLYFEVVSTFALVIGLVAGHIFNPGSGFNVDVNSIDAKAVAQY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHH
AAKAQSSSTVDFLLNIIPSTVVDAFAKGDILQILLIALLFGGALSAMGERAQMVTDFIDQ
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVVSLVPLLKLIGTFYLTAIIFVVVVLGI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARLTGFSIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVIFISQALNIELTLTQQLTILAVAMLTSKGASGITGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISAWERELDRTKLARVMSGDRGE
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCC
TVSATPAA
CCCCCCCH
>Mature Secondary Structure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CCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852