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The map label for this gene is thiD [H]

Identifier: 17544819

GI number: 17544819

Start: 112778

End: 113683

Strand: Direct

Name: thiD [H]

Synonym: RSc0100

Alternate gene names: 17544819

Gene position: 112778-113683 (Clockwise)

Preceding gene: 17544818

Following gene: 17544820

Centisome position: 3.03

GC content: 72.41

Gene sequence:

>906_bases
ATGACGATCACCGACCCGACGCTCGCCATTGTCGCTCCCGCTTCGCACGTGCCGCGCGTGCTCACCATTGCCGGCTCGGA
CAGCGGCGGCGGCGCCGGCATCCAGGCCGACCTGAAGACCTTCGCCGCGCTCGGCTGCTACGGCATGTCGGCCATCACCG
CCGTCACGGCGCAGAACACGCTGGGCGTGGCGGCGGTCGAGTCGCTCACGCCGGACATCGTCGGCGCGCAGATCGATGCC
GTGGCACAGGACATCGGTGTCGATGCCGCCAAGACCGGCATGCTGGGCTCGCCGGCGGTGGTCGAGGCGATCGTCTCGGC
GCTGGCGCGCCATCCGATCGCCGCGCTGGTGGTCGATCCCGTGATGGTCAGCACCAGCGGCGCCCAGCTCGGCAGCGATG
CCACCGCGCAGGCCATGGCGAAATGGCTGTTTCCGCGTGCGCTTCTGATCACACCAAACCTGCCCGAAGCGAGCGCGCTG
CTCGGCCGCGCGGTCCGCACCGCCGACGACATGCTGCCCGCCGCGCGCGATCTGCTGACACTGGGGTCGCGCGCCGTGCT
GCTCAAGGGCGGCCACCTCGCCGATGTGGCCATCCCCGGCGAGGACGGCGTGCTGCAGGACGTGCTGGTCACCGCCGACG
GCATCGAGCGCATCTACACGCACACCCAGATCGACACGCCGCACACGCACGGTACGGGTTGCACGCTGTCGGCGGCCATC
GCCGCGCACCTGGCGCGCGGCGAGCCGCTGGAGGCGGCGGTCGAGCTGTCGCTGGATTACCTGCTGCACGCCATCGGCGC
CGGCCGGCACCTGGCCCTGGGCCGTGGCGCCGGTCCGCTGAATCACGGCTTTGCGCCGCGCCCGCTGGCCGCGCCACGCG
CGGTGGACGTGGGCGACGCGGACTGA

Upstream 100 bases:

>100_bases
TCGGCGGACTACACCGACCTGATGGATTGACGCATCGGCGGAGCCACCGGTGGGACCGCGTCGGCGCTATAGTGCGGGTA
TCCCCAAGCCCGCCTCCGCC

Downstream 100 bases:

>100_bases
TTCCGCCGATGCCGCGGGCAGCGATGGAGGCACCATGACATCCGCCCGGATCACGCTTTCCCGCTGCCTTGCCGGCACCG
CGCTATGCCTGCTGGCGGGC

Product: bifunctional hydroxy-phosphomethylpyrimidine kinase/hydroxy-methylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTITDPTLAIVAPASHVPRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITAVTAQNTLGVAAVESLTPDIVGAQIDA
VAQDIGVDAAKTGMLGSPAVVEAIVSALARHPIAALVVDPVMVSTSGAQLGSDATAQAMAKWLFPRALLITPNLPEASAL
LGRAVRTADDMLPAARDLLTLGSRAVLLKGGHLADVAIPGEDGVLQDVLVTADGIERIYTHTQIDTPHTHGTGCTLSAAI
AAHLARGEPLEAAVELSLDYLLHAIGAGRHLALGRGAGPLNHGFAPRPLAAPRAVDVGDAD

Sequences:

>Translated_301_residues
MTITDPTLAIVAPASHVPRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITAVTAQNTLGVAAVESLTPDIVGAQIDA
VAQDIGVDAAKTGMLGSPAVVEAIVSALARHPIAALVVDPVMVSTSGAQLGSDATAQAMAKWLFPRALLITPNLPEASAL
LGRAVRTADDMLPAARDLLTLGSRAVLLKGGHLADVAIPGEDGVLQDVLVTADGIERIYTHTQIDTPHTHGTGCTLSAAI
AAHLARGEPLEAAVELSLDYLLHAIGAGRHLALGRGAGPLNHGFAPRPLAAPRAVDVGDAD
>Mature_300_residues
TITDPTLAIVAPASHVPRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITAVTAQNTLGVAAVESLTPDIVGAQIDAV
AQDIGVDAAKTGMLGSPAVVEAIVSALARHPIAALVVDPVMVSTSGAQLGSDATAQAMAKWLFPRALLITPNLPEASALL
GRAVRTADDMLPAARDLLTLGSRAVLLKGGHLADVAIPGEDGVLQDVLVTADGIERIYTHTQIDTPHTHGTGCTLSAAIA
AHLARGEPLEAAVELSLDYLLHAIGAGRHLALGRGAGPLNHGFAPRPLAAPRAVDVGDAD

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=46.9924812030075, Blast_Score=209, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325378, Length=294, Percent_Identity=35.7142857142857, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324997, Length=294, Percent_Identity=37.4149659863946, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=294, Percent_Identity=36.0544217687075, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30277; Mature: 30146

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITDPTLAIVAPASHVPRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITAVTAQNT
CCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGVAAVESLTPDIVGAQIDAVAQDIGVDAAKTGMLGSPAVVEAIVSALARHPIAALVVDP
CCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECC
VMVSTSGAQLGSDATAQAMAKWLFPRALLITPNLPEASALLGRAVRTADDMLPAARDLLT
EEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGSRAVLLKGGHLADVAIPGEDGVLQDVLVTADGIERIYTHTQIDTPHTHGTGCTLSAAI
CCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AAHLARGEPLEAAVELSLDYLLHAIGAGRHLALGRGAGPLNHGFAPRPLAAPRAVDVGDA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
D
C
>Mature Secondary Structure 
TITDPTLAIVAPASHVPRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITAVTAQNT
CCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGVAAVESLTPDIVGAQIDAVAQDIGVDAAKTGMLGSPAVVEAIVSALARHPIAALVVDP
CCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECC
VMVSTSGAQLGSDATAQAMAKWLFPRALLITPNLPEASALLGRAVRTADDMLPAARDLLT
EEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGSRAVLLKGGHLADVAIPGEDGVLQDVLVTADGIERIYTHTQIDTPHTHGTGCTLSAAI
CCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AAHLARGEPLEAAVELSLDYLLHAIGAGRHLALGRGAGPLNHGFAPRPLAAPRAVDVGDA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]