| Definition | Ralstonia solanacearum GMI1000, complete genome. |
|---|---|
| Accession | NC_003295 |
| Length | 3,716,413 |
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The map label for this gene is slt [H]
Identifier: 17544807
GI number: 17544807
Start: 101627
End: 103579
Strand: Reverse
Name: slt [H]
Synonym: RSc0088
Alternate gene names: 17544807
Gene position: 103579-101627 (Counterclockwise)
Preceding gene: 17544809
Following gene: 17544806
Centisome position: 2.79
GC content: 68.66
Gene sequence:
>1953_bases ATGACAGTGAAGGGCAAGGCAGTATATCGCGCGGTGGCGCTGGCTTTCGCGGGCGTGATCGGCATGGGCGCGGTGGGGCC GGTGCCCCTGGCGGTGGCCGGCAAGCGGCCGGCGCTGCAGGTCATTCCGGCCAATCCGGACGATGCCTTCGTCGAGTTGC GCAACGCCGCGCGCCGCAACGACGTGGCCCGCAGCTGGGAGCTCGCGGACAGCCTGTCCGACTACCCGATCCCATCCTAC GTCCAGTATTTCCGCATCAAGCCGCAGCTGTTCGACGCCAGCGGCTACGCGCGCACCGATGCGCCGGAAGACGAGATCCG CTCGTTCCTGCAGCGCTACAAGGGCGAGGCCATCGCCGACCGCCTGCGCAACGACTGGCTGCTGGTGCTCGGCAAGAAGC GCGACTGGGCCACCTTCGACGCCGAATACCCGCAGTTCGTGCTCAAGGACGACACCCAGGTCGAGTGCTACGCGCTGCTG TCGCGCGCAATGAAGGGCCAGAACGTGGCCGCCGACGCGCGCAACACCCTGTCCGATCCCAAGGGCTACGGCGAGGGCTG CGTCGACCTGATCGGTTATCTCGCCCAGTCCGGCCAGCTCGCGCGTGCCGACGTCGCCTTCGCGGCGCGGCTGTCGCTGG AGCAGAACCTCATCACGCAGGCCGCGCGCATCGCCGCCGTGCTGCCGGAGGGCGACCGGGTCGACAACGATACGCTCGCC AACGTCACGCGCATGGCGCGCAGCGACCCGTCGCAGGCCACCGCCTACCTGATGGCGCAAAGCGGCGGCCTGTCGAAGGA AGAGCAGGGCGCGGGCTGGGGCGTGATCGGCCAGTACGCCGCCAAGAAGCAGACGCCCGATGCGCTCGACGCCTACCGCC GCCAGATGACGCTGGGCGGCGACGCCTGGCTGTCGGACGACTCGCAGGAATGGCGCGTGCGCACCGCCCTGCGCGCCGGC GACTGGAAGATGGTGCGCCAGGCCATCGATTCGATGCGCGCGCCGCTGCGCAGCCGCGATCCGGCCTGGACCTACTGGTA CGGCCGTGCGCTCAAGGCCGACGGCCGCAACGAGGACGCGAAGCAGCAGTTCCAGTCGATCGCCGGGCAGTTCAACTTCT ATGGCCAGTTGGCGCTGGAAGAGCTCGGCCAGCGCATCACCGTGCCGCAGCGCACGGCCGTGACCGATGCCGAGATCGAC CCGATGGGCCGCAACCATCCGGGCTTCGGCCGCGCCAAGCGGCTGTATGACCTGAACCTGCGTTTCGAGGGCAACCGCGA GTGGAACTGGGAGCTGCGCAACATGAGCGACCGCGAGCTGCTGGCGGCGGCGGAGTATGGCCGCAAGCTCGGCATGCTCG ACCGCACCGTCAACTCGGCCGACAAGACCCGCAACGAGCATGACTTCTCGCTGCGCTTCCCGATGCCGTTCCGCAACGTG GTCGAGCGCAACGCCGATGCCGTCGGCCTGGACGTCGCCTGGACCTACGGCCTGATCCGCCAGGAATCGCGCTTCATCAT GGACGCGCGCTCCTCGGTGGGCGCATCGGGCCTGATGCAGGTGATGCCCGAGACCGCCAAGCGCGTCGCCAAGAAGATCG GCCTGCCCGTCTTCCGTCCCTCGCAGATGAGCGACATCGATACCAACGTGCTGCTCGGCACCAGCTACCTGTCGATGATC CTGACCGACCTCGACAGCTCGATGACGCTGGCCACCGCCGGCTACAACGCCGGCCCCGGCCGGCCCAAGCGCTGGCGCTC CACGCTGACGCGCCCGGTGGAAGGCGCGATCTTCGCCGAGACCATCCCCTTCAACGAGACCCGCACCTACGTGAAGAACG TACTGTCGAACGCCACCTACTACGGTGCCCTGCTGACCGGCCGGCCGCAATCGCTGAAAGACCGCCTGGGCAAGGTCGCG CCCGAGGCCGTGACGCCGGACGATCTGCCGTAG
Upstream 100 bases:
>100_bases CGGCGCGATTCGACCGGTGTGGTCGGGTGGGCCGGATCGTGCGGGTTGCCGAAGGATGGCATGGGATAATCAGCGCACCG TAACGGTGATGGGGCAGGAA
Downstream 100 bases:
>100_bases GCCGCACCGCCATCGCATCGCCGCGCCCGCACCTGCTGCGGGCGTTTTTTTTTGCCCGCGCACAGATGCGCCATGGCGAT CACGCGGTGCCTGACGCGCG
Product: soluble lytic murein transglycosylase precursor transmembrane protein
Products: 1,6-Anhydrobond [C]
Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]
Number of amino acids: Translated: 650; Mature: 649
Protein sequence:
>650_residues MTVKGKAVYRAVALAFAGVIGMGAVGPVPLAVAGKRPALQVIPANPDDAFVELRNAARRNDVARSWELADSLSDYPIPSY VQYFRIKPQLFDASGYARTDAPEDEIRSFLQRYKGEAIADRLRNDWLLVLGKKRDWATFDAEYPQFVLKDDTQVECYALL SRAMKGQNVAADARNTLSDPKGYGEGCVDLIGYLAQSGQLARADVAFAARLSLEQNLITQAARIAAVLPEGDRVDNDTLA NVTRMARSDPSQATAYLMAQSGGLSKEEQGAGWGVIGQYAAKKQTPDALDAYRRQMTLGGDAWLSDDSQEWRVRTALRAG DWKMVRQAIDSMRAPLRSRDPAWTYWYGRALKADGRNEDAKQQFQSIAGQFNFYGQLALEELGQRITVPQRTAVTDAEID PMGRNHPGFGRAKRLYDLNLRFEGNREWNWELRNMSDRELLAAAEYGRKLGMLDRTVNSADKTRNEHDFSLRFPMPFRNV VERNADAVGLDVAWTYGLIRQESRFIMDARSSVGASGLMQVMPETAKRVAKKIGLPVFRPSQMSDIDTNVLLGTSYLSMI LTDLDSSMTLATAGYNAGPGRPKRWRSTLTRPVEGAIFAETIPFNETRTYVKNVLSNATYYGALLTGRPQSLKDRLGKVA PEAVTPDDLP
Sequences:
>Translated_650_residues MTVKGKAVYRAVALAFAGVIGMGAVGPVPLAVAGKRPALQVIPANPDDAFVELRNAARRNDVARSWELADSLSDYPIPSY VQYFRIKPQLFDASGYARTDAPEDEIRSFLQRYKGEAIADRLRNDWLLVLGKKRDWATFDAEYPQFVLKDDTQVECYALL SRAMKGQNVAADARNTLSDPKGYGEGCVDLIGYLAQSGQLARADVAFAARLSLEQNLITQAARIAAVLPEGDRVDNDTLA NVTRMARSDPSQATAYLMAQSGGLSKEEQGAGWGVIGQYAAKKQTPDALDAYRRQMTLGGDAWLSDDSQEWRVRTALRAG DWKMVRQAIDSMRAPLRSRDPAWTYWYGRALKADGRNEDAKQQFQSIAGQFNFYGQLALEELGQRITVPQRTAVTDAEID PMGRNHPGFGRAKRLYDLNLRFEGNREWNWELRNMSDRELLAAAEYGRKLGMLDRTVNSADKTRNEHDFSLRFPMPFRNV VERNADAVGLDVAWTYGLIRQESRFIMDARSSVGASGLMQVMPETAKRVAKKIGLPVFRPSQMSDIDTNVLLGTSYLSMI LTDLDSSMTLATAGYNAGPGRPKRWRSTLTRPVEGAIFAETIPFNETRTYVKNVLSNATYYGALLTGRPQSLKDRLGKVA PEAVTPDDLP >Mature_649_residues TVKGKAVYRAVALAFAGVIGMGAVGPVPLAVAGKRPALQVIPANPDDAFVELRNAARRNDVARSWELADSLSDYPIPSYV QYFRIKPQLFDASGYARTDAPEDEIRSFLQRYKGEAIADRLRNDWLLVLGKKRDWATFDAEYPQFVLKDDTQVECYALLS RAMKGQNVAADARNTLSDPKGYGEGCVDLIGYLAQSGQLARADVAFAARLSLEQNLITQAARIAAVLPEGDRVDNDTLAN VTRMARSDPSQATAYLMAQSGGLSKEEQGAGWGVIGQYAAKKQTPDALDAYRRQMTLGGDAWLSDDSQEWRVRTALRAGD WKMVRQAIDSMRAPLRSRDPAWTYWYGRALKADGRNEDAKQQFQSIAGQFNFYGQLALEELGQRITVPQRTAVTDAEIDP MGRNHPGFGRAKRLYDLNLRFEGNREWNWELRNMSDRELLAAAEYGRKLGMLDRTVNSADKTRNEHDFSLRFPMPFRNVV ERNADAVGLDVAWTYGLIRQESRFIMDARSSVGASGLMQVMPETAKRVAKKIGLPVFRPSQMSDIDTNVLLGTSYLSMIL TDLDSSMTLATAGYNAGPGRPKRWRSTLTRPVEGAIFAETIPFNETRTYVKNVLSNATYYGALLTGRPQSLKDRLGKVAP EAVTPDDLP
Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]
COG id: COG0741
COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)
Gene ontology:
Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transglycosylase slt family [H]
Homologues:
Organism=Escherichia coli, GI87082441, Length=324, Percent_Identity=30.2469135802469, Blast_Score=150, Evalue=2e-37,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016026 - InterPro: IPR008258 - InterPro: IPR012289 - InterPro: IPR008939 - InterPro: IPR000189 [H]
Pfam domain/function: PF01464 SLT [H]
EC number: 3.2.1.- [C]
Molecular weight: Translated: 72187; Mature: 72056
Theoretical pI: Translated: 9.22; Mature: 9.22
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTVKGKAVYRAVALAFAGVIGMGAVGPVPLAVAGKRPALQVIPANPDDAFVELRNAARRN CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHH DVARSWELADSLSDYPIPSYVQYFRIKPQLFDASGYARTDAPEDEIRSFLQRYKGEAIAD HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHH RLRNDWLLVLGKKRDWATFDAEYPQFVLKDDTQVECYALLSRAMKGQNVAADARNTLSDP HHCCCEEEEEECCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC KGYGEGCVDLIGYLAQSGQLARADVAFAARLSLEQNLITQAARIAAVLPEGDRVDNDTLA CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH NVTRMARSDPSQATAYLMAQSGGLSKEEQGAGWGVIGQYAAKKQTPDALDAYRRQMTLGG HHHHHHHCCHHHHHEEEEECCCCCCCHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCC DAWLSDDSQEWRVRTALRAGDWKMVRQAIDSMRAPLRSRDPAWTYWYGRALKADGRNEDA CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCHHH KQQFQSIAGQFNFYGQLALEELGQRITVPQRTAVTDAEIDPMGRNHPGFGRAKRLYDLNL HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE RFEGNREWNWELRNMSDRELLAAAEYGRKLGMLDRTVNSADKTRNEHDFSLRFPMPFRNV EEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH VERNADAVGLDVAWTYGLIRQESRFIMDARSSVGASGLMQVMPETAKRVAKKIGLPVFRP HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC SQMSDIDTNVLLGTSYLSMILTDLDSSMTLATAGYNAGPGRPKRWRSTLTRPVEGAIFAE CCCCCCCCHHEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEE TIPFNETRTYVKNVLSNATYYGALLTGRPQSLKDRLGKVAPEAVTPDDLP CCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCC >Mature Secondary Structure TVKGKAVYRAVALAFAGVIGMGAVGPVPLAVAGKRPALQVIPANPDDAFVELRNAARRN CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHH DVARSWELADSLSDYPIPSYVQYFRIKPQLFDASGYARTDAPEDEIRSFLQRYKGEAIAD HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHH RLRNDWLLVLGKKRDWATFDAEYPQFVLKDDTQVECYALLSRAMKGQNVAADARNTLSDP HHCCCEEEEEECCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC KGYGEGCVDLIGYLAQSGQLARADVAFAARLSLEQNLITQAARIAAVLPEGDRVDNDTLA CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH NVTRMARSDPSQATAYLMAQSGGLSKEEQGAGWGVIGQYAAKKQTPDALDAYRRQMTLGG HHHHHHHCCHHHHHEEEEECCCCCCCHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCC DAWLSDDSQEWRVRTALRAGDWKMVRQAIDSMRAPLRSRDPAWTYWYGRALKADGRNEDA CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCHHH KQQFQSIAGQFNFYGQLALEELGQRITVPQRTAVTDAEIDPMGRNHPGFGRAKRLYDLNL HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE RFEGNREWNWELRNMSDRELLAAAEYGRKLGMLDRTVNSADKTRNEHDFSLRFPMPFRNV EEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH VERNADAVGLDVAWTYGLIRQESRFIMDARSSVGASGLMQVMPETAKRVAKKIGLPVFRP HHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC SQMSDIDTNVLLGTSYLSMILTDLDSSMTLATAGYNAGPGRPKRWRSTLTRPVEGAIFAE CCCCCCCCHHEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEE TIPFNETRTYVKNVLSNATYYGALLTGRPQSLKDRLGKVAPEAVTPDDLP CCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]
Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]
General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]