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The map label for this gene is def

Identifier: 17544789

GI number: 17544789

Start: 80019

End: 80528

Strand: Direct

Name: def

Synonym: RSc0070

Alternate gene names: 17544789

Gene position: 80019-80528 (Clockwise)

Preceding gene: 17544784

Following gene: 17544790

Centisome position: 2.15

GC content: 63.53

Gene sequence:

>510_bases
ATGGCCCTACTGAATATCCTGCAATACCCCGATCCGCGACTGCACAAGGTCGCCAAGCCGGTGGCCGTCGTGGACGACCG
CATCCGCAAGCTGGTGGCCGATATGGCCGAGACGATGTATGACGCGCCCGGCATCGGCCTGGCCGCCACGCAGGTCGACG
TGCACGAGCGGGTGATCACGATCGACGTATCGGAATCGCGCGACGAGCTGCGCGTCTTCATCAACCCGGAGATCGTCTGG
GCGAGCGAGGCGCGCAAGGTCTGGGACGAAGGCTGCCTGTCGGTGCCCGACATCTACGACAAGGTGGAGCGGCCGGACCG
GGTGCGCGTGCGTGCGCTCAACGAGAAGGGCGAATCCTTCGAGCTGGAAACCGACGGGCTGCTGGCGGTTTGCATCCAGC
ATGAGATGGATCACCTGATGGGCAAGGTGTTCGTCGAGTACCTGTCACCGCTCAAGCAGAACCGCATCAAGATCAAGCTC
AAGAAGCACCAGCTCGAGCGCGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCGGATAGCGCCCCCGATTCTGCATGACATTCGAAGCGACCGCAATTGACGGCGATCCGCCGTCTGCGTGGCCTTCCGC
AACCGTTGTTGCCAAGCATC

Downstream 100 bases:

>100_bases
ATTTCGTGACGCCCGATGCCGAAGCGCCCCCCCGTTCCGTGCCGTTGATGACGCTGTCCGAAGCCGACGGCGGTGCCGCT
TCGCGCCGTCTGCCGCTGCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MALLNILQYPDPRLHKVAKPVAVVDDRIRKLVADMAETMYDAPGIGLAATQVDVHERVITIDVSESRDELRVFINPEIVW
ASEARKVWDEGCLSVPDIYDKVERPDRVRVRALNEKGESFELETDGLLAVCIQHEMDHLMGKVFVEYLSPLKQNRIKIKL
KKHQLERAR

Sequences:

>Translated_169_residues
MALLNILQYPDPRLHKVAKPVAVVDDRIRKLVADMAETMYDAPGIGLAATQVDVHERVITIDVSESRDELRVFINPEIVW
ASEARKVWDEGCLSVPDIYDKVERPDRVRVRALNEKGESFELETDGLLAVCIQHEMDHLMGKVFVEYLSPLKQNRIKIKL
KKHQLERAR
>Mature_168_residues
ALLNILQYPDPRLHKVAKPVAVVDDRIRKLVADMAETMYDAPGIGLAATQVDVHERVITIDVSESRDELRVFINPEIVWA
SEARKVWDEGCLSVPDIYDKVERPDRVRVRALNEKGESFELETDGLLAVCIQHEMDHLMGKVFVEYLSPLKQNRIKIKLK
KHQLERAR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=163, Percent_Identity=28.8343558282209, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=56.8047337278106, Blast_Score=191, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24645728, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=29.4478527607362, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_RALSO (Q8Y3B0)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518191.1
- ProteinModelPortal:   Q8Y3B0
- SMR:   Q8Y3B0
- GeneID:   1218872
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0070
- NMPDR:   fig|267608.1.peg.70
- HOGENOM:   HBG665227
- OMA:   TRASEVT
- ProtClustDB:   PRK00150
- BioCyc:   RSOL267608:RSC0070-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19425; Mature: 19294

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLNILQYPDPRLHKVAKPVAVVDDRIRKLVADMAETMYDAPGIGLAATQVDVHERVIT
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHEEEE
IDVSESRDELRVFINPEIVWASEARKVWDEGCLSVPDIYDKVERPDRVRVRALNEKGESF
EEECCCCCCEEEEECCEEEEHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCEE
ELETDGLLAVCIQHEMDHLMGKVFVEYLSPLKQNRIKIKLKKHQLERAR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
ALLNILQYPDPRLHKVAKPVAVVDDRIRKLVADMAETMYDAPGIGLAATQVDVHERVIT
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHEEEE
IDVSESRDELRVFINPEIVWASEARKVWDEGCLSVPDIYDKVERPDRVRVRALNEKGESF
EEECCCCCCEEEEECCEEEEHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCEE
ELETDGLLAVCIQHEMDHLMGKVFVEYLSPLKQNRIKIKLKKHQLERAR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852