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The map label for this gene is rlmI [H]

Identifier: 17544770

GI number: 17544770

Start: 56123

End: 57316

Strand: Reverse

Name: rlmI [H]

Synonym: RSc0051

Alternate gene names: 17544770

Gene position: 57316-56123 (Counterclockwise)

Preceding gene: 17544771

Following gene: 17544769

Centisome position: 1.54

GC content: 71.44

Gene sequence:

>1194_bases
ATGCAAACCCTGATTCTCAAGCCCGGCAAGGAGCGCTCGCTGCTGCGCCGGCACCCGTGGATATTCGCCAACGCCATCGA
CCGCGTGGAAGGCCGCCCGGCCTCCGGCACCACCATGGTGGTCCGCGCGCATGACGGCCGCTTCCTGGCCCGCGCCGCGT
TCAGCCCCACCTCGCAGATCCGCGCGCGGGTGTGGTCCTTCGATGAAGCCGAGCCGATCGATCACGCCTTCTTCAAGCGC
CGCATCGCCGCCGCCGTGGCGTACCGGCGCGACTGGGTGCGCGACACCGACGCCGTGCGCCTGGTCATGGGCGAGGCCGA
CGGCCTGCCCGGCCTGATCGTCGACCAGTACGGCACGCTCGCCGCCGGCCAGCTGGTGTGCCAGTTCAGCGCGACCGGCG
TCGAGCACTGGAAGGAGGCGATCGTCGCCGCGCTGGTCAAGGAGACCGGCTGCCCCAACGTGTACGAACGCTCGGACGTG
AGCGTGCGCGAGCGCGAGGGCCTGCCGCAGCAGACCGGCGTGCTGGCCGGCGCCGAGCCGCCCGCCGACCTGTCGGCCAC
CGAGCACGGCGTGCGCTACTACGTGGACGCGCGCGCCGGCCACAAGACCGGCTTCTACGTGGACCAGCGCGACAACCGCG
CGCTGGTCGGCGCGCTGGCGCGGGACCGCGACGTGCTCAACTGCTTCTGCTACACCGGCGGCTTCTCGCTGGCGGCGCTC
AAGGGCGGGGCGCGCTCGGTGATCTCGATCGACTCGTCGGGCGAGGCGCTGGCGGTGGCCGAGCGCAACGTCGCGCTCAA
CGGCTTCGATGCGGCGCGCGCCGAATGGCTGGATGCCGATGTCTTCAAGTCACTGCGCAACTTCCGCGCCGAGGGCCGCC
AGTTCGACCTGATCGTGCTGGACCCGCCCAAGTTCGCCGCCTCCGCGCAGCAGGTGGACCGCGCCGCGCGAGCCTACAAA
GAGATCAACCTGGTCGGCCTGCAGTTGCTGCGGCCGGGCGGCCTGCTGTTCACGTACTCATGCTCGGGCGCCATCGACGC
CGACCTGTTCCAGAAGATCGTGGCCGGCGCGGCGGCCGATGCCCGGGTGACGGCGCGCATCCAGCGGCGGCTCTCGGCCG
GGCTGGACCATCCGCTGCTCGCCAGCTTCCCCGAAGGCGAATACCTCAAGGGCCTGCTGCTGCAGGTGGGCTGA

Upstream 100 bases:

>100_bases
CGATTTCCAGCACCTGGCCAAGATCTACGATCAGGCCCACCCGCGCGCAAAGAAGAAATGACGCGCCGCCCGGGCAACCG
GGCATTCCCATCCCAGCCGT

Downstream 100 bases:

>100_bases
CGGCGCGCCATCGCGGCCCGCGATCTGCCGTGGAATCCGGGCAGGCGAGCGACTAAGTTGGAACCTCCGGGCCCGCGCCG
CCGGCGTGCGGCCCGACCCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQTLILKPGKERSLLRRHPWIFANAIDRVEGRPASGTTMVVRAHDGRFLARAAFSPTSQIRARVWSFDEAEPIDHAFFKR
RIAAAVAYRRDWVRDTDAVRLVMGEADGLPGLIVDQYGTLAAGQLVCQFSATGVEHWKEAIVAALVKETGCPNVYERSDV
SVREREGLPQQTGVLAGAEPPADLSATEHGVRYYVDARAGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
KGGARSVISIDSSGEALAVAERNVALNGFDAARAEWLDADVFKSLRNFRAEGRQFDLIVLDPPKFAASAQQVDRAARAYK
EINLVGLQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAADARVTARIQRRLSAGLDHPLLASFPEGEYLKGLLLQVG

Sequences:

>Translated_397_residues
MQTLILKPGKERSLLRRHPWIFANAIDRVEGRPASGTTMVVRAHDGRFLARAAFSPTSQIRARVWSFDEAEPIDHAFFKR
RIAAAVAYRRDWVRDTDAVRLVMGEADGLPGLIVDQYGTLAAGQLVCQFSATGVEHWKEAIVAALVKETGCPNVYERSDV
SVREREGLPQQTGVLAGAEPPADLSATEHGVRYYVDARAGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
KGGARSVISIDSSGEALAVAERNVALNGFDAARAEWLDADVFKSLRNFRAEGRQFDLIVLDPPKFAASAQQVDRAARAYK
EINLVGLQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAADARVTARIQRRLSAGLDHPLLASFPEGEYLKGLLLQVG
>Mature_397_residues
MQTLILKPGKERSLLRRHPWIFANAIDRVEGRPASGTTMVVRAHDGRFLARAAFSPTSQIRARVWSFDEAEPIDHAFFKR
RIAAAVAYRRDWVRDTDAVRLVMGEADGLPGLIVDQYGTLAAGQLVCQFSATGVEHWKEAIVAALVKETGCPNVYERSDV
SVREREGLPQQTGVLAGAEPPADLSATEHGVRYYVDARAGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
KGGARSVISIDSSGEALAVAERNVALNGFDAARAEWLDADVFKSLRNFRAEGRQFDLIVLDPPKFAASAQQVDRAARAYK
EINLVGLQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAADARVTARIQRRLSAGLDHPLLASFPEGEYLKGLLLQVG

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=47.2222222222222, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787181, Length=218, Percent_Identity=32.5688073394495, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43241; Mature: 43241

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLILKPGKERSLLRRHPWIFANAIDRVEGRPASGTTMVVRAHDGRFLARAAFSPTSQI
CCEEEECCCCCHHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHHH
RARVWSFDEAEPIDHAFFKRRIAAAVAYRRDWVRDTDAVRLVMGEADGLPGLIVDQYGTL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCH
AAGQLVCQFSATGVEHWKEAIVAALVKETGCPNVYERSDVSVREREGLPQQTGVLAGAEP
HHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCEEECCCC
PADLSATEHGVRYYVDARAGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
CCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEHHHHCCCCEEEEEEECCCCEEEEE
KGGARSVISIDSSGEALAVAERNVALNGFDAARAEWLDADVFKSLRNFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEECCEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEE
DPPKFAASAQQVDRAARAYKEINLVGLQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAAD
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ARVTARIQRRLSAGLDHPLLASFPEGEYLKGLLLQVG
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEECC
>Mature Secondary Structure
MQTLILKPGKERSLLRRHPWIFANAIDRVEGRPASGTTMVVRAHDGRFLARAAFSPTSQI
CCEEEECCCCCHHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHHH
RARVWSFDEAEPIDHAFFKRRIAAAVAYRRDWVRDTDAVRLVMGEADGLPGLIVDQYGTL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCH
AAGQLVCQFSATGVEHWKEAIVAALVKETGCPNVYERSDVSVREREGLPQQTGVLAGAEP
HHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCEEECCCC
PADLSATEHGVRYYVDARAGHKTGFYVDQRDNRALVGALARDRDVLNCFCYTGGFSLAAL
CCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEHHHHCCCCEEEEEEECCCCEEEEE
KGGARSVISIDSSGEALAVAERNVALNGFDAARAEWLDADVFKSLRNFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEECCEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEE
DPPKFAASAQQVDRAARAYKEINLVGLQLLRPGGLLFTYSCSGAIDADLFQKIVAGAAAD
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ARVTARIQRRLSAGLDHPLLASFPEGEYLKGLLLQVG
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA