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The map label for this gene is pckA [H]

Identifier: 17544736

GI number: 17544736

Start: 18932

End: 20800

Strand: Direct

Name: pckA [H]

Synonym: RSc0017

Alternate gene names: 17544736

Gene position: 18932-20800 (Clockwise)

Preceding gene: 17544734

Following gene: 17544737

Centisome position: 0.51

GC content: 67.31

Gene sequence:

>1869_bases
ATGAATCAACCCGTGATGCAGGGTGTTCCCGCATTGAACGTGCCCGCCTATGTGAAACACGCGCGGCTGGTGGCGTGGGT
CAGCGAGATCGCTGCGCTCACCAAGCCGGAGCGCGTGGTCTGGTGCGATGGCTCGCAGGAGGAATACGACCGCCTGTGCG
CCGAGATGGTCGCCGCCGGCACGCTCAAGCAGCTCAATCCCGCCAAGCGCAAGAACTCGTACCTGGCGCTGTCCGACCCC
TCGGACGTGGCGCGTGTGGAGGATCGGACCTTCATCTGCTCGCAGAAGCAGGAAGACGCGGGCCCGACCAACAACTGGAC
CGCCCCGGCCGAGATGCGCCAGACGCTGAACGGCCTGTTCGACGGTTGCATGCGCGGTCGCACGCTGTACGTGGTGCCGT
TCTCGATGGGCCCGCTGGGCTCGCCGATCGCGCACATCGGCGTGGAGCTGTCCGACAGCCCGTATGTGGCCGTCAACATG
CGCATCATGACCCGCATGGGCCGTGCCGTGTACGACGTGCTGGGCGCCGACGGCGAGTTCGTGCCGTGCGTGCATACCGT
CGGTAAGCCGCTGGCCGCGGGTGAACAGGATGTGCCGTGGCCGTGCAACCCGACCAAGTACATCGTGCACTTCCCGGAAA
CGCGCGAGATCTGGTCGTTCGGCTCGGGCTACGGCGGCAACGCGCTGCTGGGCAAGAAGTGCTTCGCGCTGCGGATCGCT
TCCACCATGGGCCGCGACCAGGGCTGGCTGGCCGAGCACATGCTGATCCTGGGCGTGACCTCGCCCGAGGGCAAGACCTA
CCACGTGGCCGCGGCCTTCCCGTCGGCCTGCGGCAAGACCAACTTCGCGATGCTGATTCCGCCGGCCGGCTTCGACGGCT
GGAAGGTCACCACCATCGGCGACGACATCGCCTGGATCAAGCCGCGCCAGGACGCCAACGGTCAGACGCGCCTGTACGCC
ATCAACCCGGAAGCCGGTTTCTTCGGTGTGGCGCCGGGCACGGGCGAGAAGACCAACTTCAACGCGATGGCCACGCTCAA
GGAAAACGTCATCTTCACCAACGTCGCGCTTACCGATGACGGCGACGTGTGGTGGGAAGGCATGACCGACACGCCGCCCG
CGCACCTGACCGACTGGCAAGGCCAGGACTGGACCCCGGCGATCGCCAAGGAAACCGGCCGCAAGGCCGCGCACCCGAAC
TCGCGCTTCACGGCGCCGGCTGCGCAGTGCCCGTCGATCGATCCGGAGTGGGACAACCCGGCCGGCGTGGCCATCGATGC
GTTCATCTTCGGCGGCCGCCGCTCGACCACCGTGCCGCTGGTGACCGAAGCGCGCGACTGGACCGAAGGCGTGTACATGG
CCGCCACGATGGGCTCGGAAACCACCGCCGCCGCCGTCGGCCAGCAGGGCGTGGTGCGCCGCGATCCGTTCGCCATGCTG
CCGTTCTGCGGCTACAACATGGCCGACTATTTCGCGCACTGGCTCAAGCTGGGCGACCAGCTGGCCAAGAGCGGCGCCGA
GCTGCCGAAGATCTTCTGCGTCAACTGGTTCCGCAAGGATGAGCAGGGCCGCTTCGTGTGGCCGGGCTTCGGCGAGAACA
TGCGCGTGCTGAAGTGGATGATCGACCGGATCGAAGGCCAGGCTCGCGGCGACGAGCATGTCTTTGGCGTGTCGCCGCGC
TACGAGGAGCTGCGCTGGGATGGGCTGGACTTCTCCGCCGAGCAGTTCGCCAAGGTGATCTCGCTGGATGCGCAGGCCTG
GCAGCAGGAACTGACGCTGCACGCCGAGCTGTTCGCCCAGCTGGCGCATCACCTGCCGCAGGCGCTGCCGGAAGCCAAGG
CACGTCTGGAGGCCCGCCTGCAGGGCTGA

Upstream 100 bases:

>100_bases
TGCCGTCGATCGCTTTCCGGTCCGCTGACGCGGGGGTGTGCCTGGCCTGCCGTGTGGATCTTCCACCGCTTTCCTTTTCA
CTTCCATTGGAGTTTGAGTC

Downstream 100 bases:

>100_bases
TCGCCGATCGCTGCCGGATAGATGGGCTGAGCCCGCGCGTTGCGCGGGCTTTTTTTTTGCCTGGCCCGCCCTTTTTTTGG
GCAGTAATACGAAAGGCTTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNQPVMQGVPALNVPAYVKHARLVAWVSEIAALTKPERVVWCDGSQEEYDRLCAEMVAAGTLKQLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEQDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGFDGWKVTTIGDDIAWIKPRQDANGQTRLYA
INPEAGFFGVAPGTGEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDTPPAHLTDWQGQDWTPAIAKETGRKAAHPN
SRFTAPAAQCPSIDPEWDNPAGVAIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAVGQQGVVRRDPFAML
PFCGYNMADYFAHWLKLGDQLAKSGAELPKIFCVNWFRKDEQGRFVWPGFGENMRVLKWMIDRIEGQARGDEHVFGVSPR
YEELRWDGLDFSAEQFAKVISLDAQAWQQELTLHAELFAQLAHHLPQALPEAKARLEARLQG

Sequences:

>Translated_622_residues
MNQPVMQGVPALNVPAYVKHARLVAWVSEIAALTKPERVVWCDGSQEEYDRLCAEMVAAGTLKQLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEQDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGFDGWKVTTIGDDIAWIKPRQDANGQTRLYA
INPEAGFFGVAPGTGEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDTPPAHLTDWQGQDWTPAIAKETGRKAAHPN
SRFTAPAAQCPSIDPEWDNPAGVAIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAVGQQGVVRRDPFAML
PFCGYNMADYFAHWLKLGDQLAKSGAELPKIFCVNWFRKDEQGRFVWPGFGENMRVLKWMIDRIEGQARGDEHVFGVSPR
YEELRWDGLDFSAEQFAKVISLDAQAWQQELTLHAELFAQLAHHLPQALPEAKARLEARLQG
>Mature_622_residues
MNQPVMQGVPALNVPAYVKHARLVAWVSEIAALTKPERVVWCDGSQEEYDRLCAEMVAAGTLKQLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGRAVYDVLGADGEFVPCVHTVGKPLAAGEQDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGFDGWKVTTIGDDIAWIKPRQDANGQTRLYA
INPEAGFFGVAPGTGEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDTPPAHLTDWQGQDWTPAIAKETGRKAAHPN
SRFTAPAAQCPSIDPEWDNPAGVAIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAVGQQGVVRRDPFAML
PFCGYNMADYFAHWLKLGDQLAKSGAELPKIFCVNWFRKDEQGRFVWPGFGENMRVLKWMIDRIEGQARGDEHVFGVSPR
YEELRWDGLDFSAEQFAKVISLDAQAWQQELTLHAELFAQLAHHLPQALPEAKARLEARLQG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=604, Percent_Identity=50.8278145695364, Blast_Score=586, Evalue=1e-167,
Organism=Homo sapiens, GI66346721, Length=610, Percent_Identity=50.655737704918, Blast_Score=578, Evalue=1e-165,
Organism=Homo sapiens, GI66346723, Length=381, Percent_Identity=53.5433070866142, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71989645, Length=640, Percent_Identity=45.9375, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17555492, Length=626, Percent_Identity=47.444089456869, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71989658, Length=629, Percent_Identity=46.2639109697933, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564411, Length=556, Percent_Identity=51.0791366906475, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989653, Length=603, Percent_Identity=47.0978441127695, Blast_Score=533, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI32564407, Length=534, Percent_Identity=50, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71989649, Length=541, Percent_Identity=47.5046210720887, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI32564409, Length=380, Percent_Identity=47.1052631578947, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17540956, Length=527, Percent_Identity=26.1859582542694, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655085, Length=609, Percent_Identity=49.2610837438424, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24655082, Length=610, Percent_Identity=49.0163934426229, Blast_Score=569, Evalue=1e-162,
Organism=Drosophila melanogaster, GI161077226, Length=580, Percent_Identity=49.6551724137931, Blast_Score=549, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24655088, Length=513, Percent_Identity=50.8771929824561, Blast_Score=509, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68462; Mature: 68462

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPVMQGVPALNVPAYVKHARLVAWVSEIAALTKPERVVWCDGSQEEYDRLCAEMVAAG
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TLKQLNPAKRKNSYLALSDPSDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLF
HHHHCCCHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRAVYDVLGADGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEQDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHHHHH
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGFDGWKVTTIG
HHCCCCCCHHHCEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPRQDANGQTRLYAINPEAGFFGVAPGTGEKTNFNAMATLKENVIFTNVALTDD
CCEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECC
GDVWWEGMTDTPPAHLTDWQGQDWTPAIAKETGRKAAHPNSRFTAPAAQCPSIDPEWDNP
CCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
AGVAIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAVGQQGVVRRDPFAML
CCEEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCCEECCCCEEC
PFCGYNMADYFAHWLKLGDQLAKSGAELPKIFCVNWFRKDEQGRFVWPGFGENMRVLKWM
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHEEECCCCCEECCCCCCCHHHHHHH
IDRIEGQARGDEHVFGVSPRYEELRWDGLDFSAEQFAKVISLDAQAWQQELTLHAELFAQ
HHHHCCCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHHLPQALPEAKARLEARLQG
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQPVMQGVPALNVPAYVKHARLVAWVSEIAALTKPERVVWCDGSQEEYDRLCAEMVAAG
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TLKQLNPAKRKNSYLALSDPSDVARVEDRTFICSQKQEDAGPTNNWTAPAEMRQTLNGLF
HHHHCCCHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRAVYDVLGADGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEQDVPWPCNPTKYIVHFPETREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHHHHH
STMGRDQGWLAEHMLILGVTSPEGKTYHVAAAFPSACGKTNFAMLIPPAGFDGWKVTTIG
HHCCCCCCHHHCEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPRQDANGQTRLYAINPEAGFFGVAPGTGEKTNFNAMATLKENVIFTNVALTDD
CCEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECC
GDVWWEGMTDTPPAHLTDWQGQDWTPAIAKETGRKAAHPNSRFTAPAAQCPSIDPEWDNP
CCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
AGVAIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAVGQQGVVRRDPFAML
CCEEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCCEECCCCEEC
PFCGYNMADYFAHWLKLGDQLAKSGAELPKIFCVNWFRKDEQGRFVWPGFGENMRVLKWM
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHEEECCCCCEECCCCCCCHHHHHHH
IDRIEGQARGDEHVFGVSPRYEELRWDGLDFSAEQFAKVISLDAQAWQQELTLHAELFAQ
HHHHCCCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHHLPQALPEAKARLEARLQG
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA