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The map label for this gene is cca [H]

Identifier: 161621735

GI number: 161621735

Start: 98292

End: 99518

Strand: Reverse

Name: cca [H]

Synonym: RSc0085

Alternate gene names: 161621735

Gene position: 99518-98292 (Counterclockwise)

Preceding gene: 17544805

Following gene: 17544788

Centisome position: 2.68

GC content: 73.51

Gene sequence:

>1227_bases
GTGTATGCCGTGGGCGGCGCCATTCGCGACACCCTGCTGGGCCTGCCCGTGCAGGACCGCGATTACGTCGTCGTCGGCGC
CACGCCCGAGGCGATGGAGGCGCGCGGCTTCCGCACCGTGGGCAAGGATTTCCCGGTCTTCCTGCACCCGCGCACGCAGG
CCGAGTACGCGCTGGCGCGCACCGAGCGCAAGACGGCGGCCGGCTACAAGGGCTTCTCGGTCTACTACGCCCCCGACGTC
ACGCTCGAAGACGACCTGGTCCGCCGCGACCTGACCATCAACGCGATGGCGCAGCGCGTGGCGGAGGACGGCGCGCTGGT
CGGCCCCGTCATCGACCCGTACGGCGGGCAGGCGGATCTCGCCTCGCGCACCTTCCGCCACGTCTCCGAGGCCTTCGTCG
AAGACCCGGTGCGCATCCTGCGCGTGGCGCGCTTTGCCGCGCGCTTCGCCGAGTTCCACGTCGCCCCCGAGACCCGCGCG
CTGATGCAGCGCATGGCCGAGGCCGGCGAGGTGGACGCGCTGGTGCCCGAGCGGGTCTGGCAGGAGCTGGCGCGCGGGCT
GCTGGAAGCGCGGCCGTCGCGCCTGTTCGCCGTGCTGCGCGACTGCGGTGCGCTGGTCCGCCTGCTGCCCGAGCTCGACC
GGCTGTGGGGCGTGCCGCAGCGCGCCGACTATCACCCCGAGGTCGACACCGGCGTGCACACCATGATGGTCGTCGACACC
GCCGCCGCCATGGACACGCCGCTGCCGGTGCGCTTTGCCGCGCTGGTGCACGATCTCGGCAAGGGCACCACGCCCGCCGA
CATCCTGCCGCGCCACGTCGGCCACGAAGCGCGCGGCCTGCCAATGATCGAAGACATCTGCCGGCGCCTGCGCGTGCCCA
CCGACTGCCGCGACCTGGCCATCATGGTGGCGCGCGAGCACGGCAACATTCACCGCAGCGACGGGTTCGACGCCACCGCG
CTGGTGCGCCTGCTGGAGCGCTGCGACGCGCTGCGCAAACCCGAGCGCTTCCGCCAGGCGCTGCTGGCGTGCGAGGCCGA
CGCGCGCGGCCGCCTGGGCTTCGAGCAGCGCGACTACCCGCAGCCCGTGCGGCTGCTGCGGGCCCTGCAGGCCGCCGCAT
CGATCGATGCCGGCGCCGTCGCCAAGCGCTACGCCGACAACCCCGCGCACATCAAGCAGGCCGTGCACGTGGCCCGCATC
GAGGCCGTCGCGCAGGCCGGCCTCTGA

Upstream 100 bases:

>100_bases
CCGCGCTGGACGAAGACACGCTGGTCGCCGCCAACCAATGACGCAATACGCAGCATCGGCCGGTGACGCCATCGTCGATC
CGGCCACGCACGGGCTCGAC

Downstream 100 bases:

>100_bases
TGCCTACTCGAACGGCGTGTCGTTCGGACTGGTGTACAGCGCCTTGAGCTGCTCGCTCATCGGGAAGCCGAAGTTGACGC
CCTTGGGCGGCACCGGCTTC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MYAVGGAIRDTLLGLPVQDRDYVVVGATPEAMEARGFRTVGKDFPVFLHPRTQAEYALARTERKTAAGYKGFSVYYAPDV
TLEDDLVRRDLTINAMAQRVAEDGALVGPVIDPYGGQADLASRTFRHVSEAFVEDPVRILRVARFAARFAEFHVAPETRA
LMQRMAEAGEVDALVPERVWQELARGLLEARPSRLFAVLRDCGALVRLLPELDRLWGVPQRADYHPEVDTGVHTMMVVDT
AAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARGLPMIEDICRRLRVPTDCRDLAIMVAREHGNIHRSDGFDATA
LVRLLERCDALRKPERFRQALLACEADARGRLGFEQRDYPQPVRLLRALQAAASIDAGAVAKRYADNPAHIKQAVHVARI
EAVAQAGL

Sequences:

>Translated_408_residues
MYAVGGAIRDTLLGLPVQDRDYVVVGATPEAMEARGFRTVGKDFPVFLHPRTQAEYALARTERKTAAGYKGFSVYYAPDV
TLEDDLVRRDLTINAMAQRVAEDGALVGPVIDPYGGQADLASRTFRHVSEAFVEDPVRILRVARFAARFAEFHVAPETRA
LMQRMAEAGEVDALVPERVWQELARGLLEARPSRLFAVLRDCGALVRLLPELDRLWGVPQRADYHPEVDTGVHTMMVVDT
AAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARGLPMIEDICRRLRVPTDCRDLAIMVAREHGNIHRSDGFDATA
LVRLLERCDALRKPERFRQALLACEADARGRLGFEQRDYPQPVRLLRALQAAASIDAGAVAKRYADNPAHIKQAVHVARI
EAVAQAGL
>Mature_408_residues
MYAVGGAIRDTLLGLPVQDRDYVVVGATPEAMEARGFRTVGKDFPVFLHPRTQAEYALARTERKTAAGYKGFSVYYAPDV
TLEDDLVRRDLTINAMAQRVAEDGALVGPVIDPYGGQADLASRTFRHVSEAFVEDPVRILRVARFAARFAEFHVAPETRA
LMQRMAEAGEVDALVPERVWQELARGLLEARPSRLFAVLRDCGALVRLLPELDRLWGVPQRADYHPEVDTGVHTMMVVDT
AAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARGLPMIEDICRRLRVPTDCRDLAIMVAREHGNIHRSDGFDATA
LVRLLERCDALRKPERFRQALLACEADARGRLGFEQRDYPQPVRLLRALQAAASIDAGAVAKRYADNPAHIKQAVHVARI
EAVAQAGL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=407, Percent_Identity=53.8083538083538, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87081691, Length=347, Percent_Identity=27.0893371757925, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45082; Mature: 45082

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAVGGAIRDTLLGLPVQDRDYVVVGATPEAMEARGFRTVGKDFPVFLHPRTQAEYALAR
CCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCHHHCCCCCEEECCCCHHHHHHHH
TERKTAAGYKGFSVYYAPDVTLEDDLVRRDLTINAMAQRVAEDGALVGPVIDPYGGQADL
HHHHHHCCCCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCCEECCCCCCCCCCHHH
ASRTFRHVSEAFVEDPVRILRVARFAARFAEFHVAPETRALMQRMAEAGEVDALVPERVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
QELARGLLEARPSRLFAVLRDCGALVRLLPELDRLWGVPQRADYHPEVDTGVHTMMVVDT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEHH
AAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARGLPMIEDICRRLRVPTDCRDLA
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHH
IMVAREHGNIHRSDGFDATALVRLLERCDALRKPERFRQALLACEADARGRLGFEQRDYP
HHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QPVRLLRALQAAASIDAGAVAKRYADNPAHIKQAVHVARIEAVAQAGL
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYAVGGAIRDTLLGLPVQDRDYVVVGATPEAMEARGFRTVGKDFPVFLHPRTQAEYALAR
CCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCHHHCCCCCEEECCCCHHHHHHHH
TERKTAAGYKGFSVYYAPDVTLEDDLVRRDLTINAMAQRVAEDGALVGPVIDPYGGQADL
HHHHHHCCCCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCCEECCCCCCCCCCHHH
ASRTFRHVSEAFVEDPVRILRVARFAARFAEFHVAPETRALMQRMAEAGEVDALVPERVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
QELARGLLEARPSRLFAVLRDCGALVRLLPELDRLWGVPQRADYHPEVDTGVHTMMVVDT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEHH
AAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARGLPMIEDICRRLRVPTDCRDLA
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHH
IMVAREHGNIHRSDGFDATALVRLLERCDALRKPERFRQALLACEADARGRLGFEQRDYP
HHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QPVRLLRALQAAASIDAGAVAKRYADNPAHIKQAVHVARIEAVAQAGL
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]