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The map label for this gene is murD

Identifier: 161621732

GI number: 161621732

Start: 3061730

End: 3063229

Strand: Reverse

Name: murD

Synonym: RSc2846

Alternate gene names: 161621732

Gene position: 3063229-3061730 (Counterclockwise)

Preceding gene: 17547566

Following gene: 17547564

Centisome position: 82.42

GC content: 72.93

Gene sequence:

>1500_bases
ATGTTCGGCGACCTGGCGTCGCCGTTCGTGCTGGTACTGGGCCTGGGCGAGTCCGGCCTGGCCATGGCGCGCTGGTGCGC
GCGCCATGGCGCGCGCGCGCGCGTGGCCGATACGCGCGAGGCGCCCGCCAACCTGCCGGCGCTGCGCGCGCATGTGCCGG
ATGCCGAATTCATCGGCGGGCCGTTCGCTCCGTCGCTGCTGGAAGGCGTGGCGCTGGTGGCGATCAGCCCCGGCCTGTCG
CCGCTGGATGCTGCCGTTGCCGCGCTGCTGGACGGCGCACGCGAGCGCGCGGTGCCGGTGTGGGGCGAGATCGAACTGTT
CGCGCGCGCGCTGGCCGGCCTGAAGCTGGCGCAGGGCTATGCGCCCCGCGTCCTGGCCATCACCGGCACCAACGGCAAGA
CCACCACCACGGCGCTGGCGGGTGCCCTGGTCCAGCGCGCCGGCAAGACCGTCGGCGTGGCGGGCAACATCAGCCCCTCG
GCGCTGGACAAGCTGACCGAGTGCGTCGACGCCGGCACGTTGCCGGACGTGTGGGTGCTGGAGCTCTCCAGCTTCCAGCT
GGAAACCACGCACACGCTCGACGCCGACGCCGCGACCATCCTCAACATCACGCAGGACCACCTGGACTGGCACGGCTCGA
TGGCCGCCTACGCCGCCGCCAAGGGCCGCATTTTCGGTGCCGGCACGGTGCGCGTGCTGAACCGGCAGGACGCCGACGTG
ATGGCCTTCGCCGGCAAGCGTGGCGGCGATGTCACGTTCGGCACCGACGAACCGGCCACGCCCGAAGCGCTCGGCCTGCT
GCGCGACGGCGGCATCCCGTGGATCGTGCTGGCCGAAGCCGACGATGACGATCTGCCGAAGCCCGCGCGCCGCAAGAAGG
GCGACACCACGCCCGCCGCGCCGGTGCCGGTGCGGCTCAAGCGCCTGATGCCGGCCGATGCCCTGCGCATCCGCGGCCTG
CACAACGCCACCAATGCGATGGCCGCGCTGGCGCTGTGCCGCGCGATCGGCCTGCCGGCGAGCGCCTTGCTGCACGGCCT
GCGCGACTACGCCGGCGAGCCGCACCGCGTCGAACTGATCGCCGCCTTCGACGACATCGAATTCTTCGACGACAGCAAGG
GCACCAACGTCGGCGCCACGGTCGCGGCGCTGTCGGGCCTGTCCAAGCGCGTGGTGCTCATCGCCGGCGGCGACGGCAAG
GGGCAGGACTTCTCGCCGCTGGCCGCGCCGGTGGCGCAGTACGCGCGCGCCGTGGTGCTGATCGGCCGCGATGCACCGCG
GATCCGCGCCGCGCTGGCGGACAGCGGCGTCGAACTGGTGGAGGCCGCCACGCTGGAGGCCGCCGTGCAGGAAGCGGCCG
CGCGCGCGCAGGCAGGGGATGCCGTGCTGCTGTCGCCGGCCTGCGCGAGCTTCGACATGTTCCGCAACTACGAACACCGC
GCGCAGGTGTTCCACGAAGCCGTGGCCGCGCTGGCGGCCGATCGGGGAGTGATGCTATGA

Upstream 100 bases:

>100_bases
GCCGCCGGCCGCCGGGGACGCGCTCATCGCCGGCGCCATTGCTGCGACCGTCGATTCGGCCGAGCCGATGCCCGCCGCCG
AAACCGCGCAGGCGCCGCGC

Downstream 100 bases:

>100_bases
GCGACGCCCAGATCAAGCGCAGCGGCTTCATCGGCGCCACCATCGGCAACGCCTGGGAAGGGCTGCGCGACGCCGTGTCG
GGCGTCAAGCCCACGCGCTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MFGDLASPFVLVLGLGESGLAMARWCARHGARARVADTREAPANLPALRAHVPDAEFIGGPFAPSLLEGVALVAISPGLS
PLDAAVAALLDGARERAVPVWGEIELFARALAGLKLAQGYAPRVLAITGTNGKTTTTALAGALVQRAGKTVGVAGNISPS
ALDKLTECVDAGTLPDVWVLELSSFQLETTHTLDADAATILNITQDHLDWHGSMAAYAAAKGRIFGAGTVRVLNRQDADV
MAFAGKRGGDVTFGTDEPATPEALGLLRDGGIPWIVLAEADDDDLPKPARRKKGDTTPAAPVPVRLKRLMPADALRIRGL
HNATNAMAALALCRAIGLPASALLHGLRDYAGEPHRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKRVVLIAGGDGK
GQDFSPLAAPVAQYARAVVLIGRDAPRIRAALADSGVELVEAATLEAAVQEAAARAQAGDAVLLSPACASFDMFRNYEHR
AQVFHEAVAALAADRGVML

Sequences:

>Translated_499_residues
MFGDLASPFVLVLGLGESGLAMARWCARHGARARVADTREAPANLPALRAHVPDAEFIGGPFAPSLLEGVALVAISPGLS
PLDAAVAALLDGARERAVPVWGEIELFARALAGLKLAQGYAPRVLAITGTNGKTTTTALAGALVQRAGKTVGVAGNISPS
ALDKLTECVDAGTLPDVWVLELSSFQLETTHTLDADAATILNITQDHLDWHGSMAAYAAAKGRIFGAGTVRVLNRQDADV
MAFAGKRGGDVTFGTDEPATPEALGLLRDGGIPWIVLAEADDDDLPKPARRKKGDTTPAAPVPVRLKRLMPADALRIRGL
HNATNAMAALALCRAIGLPASALLHGLRDYAGEPHRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKRVVLIAGGDGK
GQDFSPLAAPVAQYARAVVLIGRDAPRIRAALADSGVELVEAATLEAAVQEAAARAQAGDAVLLSPACASFDMFRNYEHR
AQVFHEAVAALAADRGVML
>Mature_499_residues
MFGDLASPFVLVLGLGESGLAMARWCARHGARARVADTREAPANLPALRAHVPDAEFIGGPFAPSLLEGVALVAISPGLS
PLDAAVAALLDGARERAVPVWGEIELFARALAGLKLAQGYAPRVLAITGTNGKTTTTALAGALVQRAGKTVGVAGNISPS
ALDKLTECVDAGTLPDVWVLELSSFQLETTHTLDADAATILNITQDHLDWHGSMAAYAAAKGRIFGAGTVRVLNRQDADV
MAFAGKRGGDVTFGTDEPATPEALGLLRDGGIPWIVLAEADDDDLPKPARRKKGDTTPAAPVPVRLKRLMPADALRIRGL
HNATNAMAALALCRAIGLPASALLHGLRDYAGEPHRVELIAAFDDIEFFDDSKGTNVGATVAALSGLSKRVVLIAGGDGK
GQDFSPLAAPVAQYARAVVLIGRDAPRIRAALADSGVELVEAATLEAAVQEAAARAQAGDAVLLSPACASFDMFRNYEHR
AQVFHEAVAALAADRGVML

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=486, Percent_Identity=37.8600823045267, Blast_Score=228, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_RALSO (Q8XVI5)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520967.2
- ProteinModelPortal:   Q8XVI5
- SMR:   Q8XVI5
- GeneID:   1221694
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2846
- NMPDR:   fig|267608.1.peg.2846
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02006
- BioCyc:   RSOL267608:RSC2846-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51768; Mature: 51768

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDLASPFVLVLGLGESGLAMARWCARHGARARVADTREAPANLPALRAHVPDAEFIGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCHHEECCCCHHHCCC
PFAPSLLEGVALVAISPGLSPLDAAVAALLDGARERAVPVWGEIELFARALAGLKLAQGY
CCCHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
APRVLAITGTNGKTTTTALAGALVQRAGKTVGVAGNISPSALDKLTECVDAGTLPDVWVL
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
ELSSFQLETTHTLDADAATILNITQDHLDWHGSMAAYAAAKGRIFGAGTVRVLNRQDADV
EECCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCEEEEECCCCCCE
MAFAGKRGGDVTFGTDEPATPEALGLLRDGGIPWIVLAEADDDDLPKPARRKKGDTTPAA
EEECCCCCCCEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCC
PVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPASALLHGLRDYAGEPHRVELI
CCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
AAFDDIEFFDDSKGTNVGATVAALSGLSKRVVLIAGGDGKGQDFSPLAAPVAQYARAVVL
EEECCCHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEE
IGRDAPRIRAALADSGVELVEAATLEAAVQEAAARAQAGDAVLLSPACASFDMFRNYEHR
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCHHHH
AQVFHEAVAALAADRGVML
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGDLASPFVLVLGLGESGLAMARWCARHGARARVADTREAPANLPALRAHVPDAEFIGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCHHEECCCCHHHCCC
PFAPSLLEGVALVAISPGLSPLDAAVAALLDGARERAVPVWGEIELFARALAGLKLAQGY
CCCHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
APRVLAITGTNGKTTTTALAGALVQRAGKTVGVAGNISPSALDKLTECVDAGTLPDVWVL
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
ELSSFQLETTHTLDADAATILNITQDHLDWHGSMAAYAAAKGRIFGAGTVRVLNRQDADV
EECCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCEEEEECCCCCCE
MAFAGKRGGDVTFGTDEPATPEALGLLRDGGIPWIVLAEADDDDLPKPARRKKGDTTPAA
EEECCCCCCCEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCC
PVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPASALLHGLRDYAGEPHRVELI
CCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
AAFDDIEFFDDSKGTNVGATVAALSGLSKRVVLIAGGDGKGQDFSPLAAPVAQYARAVVL
EEECCCHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEE
IGRDAPRIRAALADSGVELVEAATLEAAVQEAAARAQAGDAVLLSPACASFDMFRNYEHR
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCHHHH
AQVFHEAVAALAADRGVML
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852