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The map label for this gene is sigA

Identifier: 17232755

GI number: 17232755

Start: 6277667

End: 6278839

Strand: Reverse

Name: sigA

Synonym: all5263

Alternate gene names: 17232755

Gene position: 6278839-6277667 (Counterclockwise)

Preceding gene: 17232756

Following gene: 17232750

Centisome position: 97.9

GC content: 45.44

Gene sequence:

>1173_bases
ATGAACCAGGCTAACAACGTACTCGATAGCATTTATCAGCCTGACCTAGAAATAATGAATCAGCCTGAAATCGAGTTAGA
TGACCTCCTCATTGAAGAAGATGAGGATTTGTTGCTCGCTGATGATGGCGACATTGATGAGTTTTTAGAGCCTCAGACTG
ATGAGGACGACGCAAAGTCTGGAAAAGCCGCTAAGTCGCGTCGTCGGACACAAAGCAAGAAAAAGCACTACACAGAAGAT
TCTATTCGTCTGTATTTGCAAGAAATTGGTCGAATTCGTTTGTTGCGGGCAGATGAAGAAATTGAACTTGCTAGAAAAAT
CGCTGACTTATTAGAATTAGAACGGGTGCGGGAGCGACTGTCAGAAAAGTTAGAACGTGATCCCCGTGACAGTGAGTGGG
CAGAAGCCGTACAATTACCCTTGCCAGCTTTTCGTTATCGCTTGCACATTGGCCGCAGAGCAAAAGATAAAATGGTGCAA
TCAAACTTGCGCCTTGTGGTTTCAATTGCCAAGAAGTATATGAATCGTGGTTTATCATTCCAGGATTTGATTCAGGAAGG
TAGCCTTGGTTTGATTCGTGCGGCTGAGAAGTTCGACCATGAGAAAGGTTATAAATTCTCCACCTACGCTACTTGGTGGA
TTCGCCAGGCAATCACTAGAGCGATCGCCGACCAATCCCGCACTATCCGCCTACCAGTCCACCTCTACGAAACCATCTCC
CGGATTAAGAAAACCACCAAGTTGCTGTCTCAAGAAATGGGACGCAAACCTACGGAAGAAGAAATCGCTACTCGCATGGA
AATGACCATCGAGAAACTGCGGTTCATCGCTAAATCTGCTCAATTACCAATTTCATTAGAAACACCCATTGGTAAAGAAG
AAGATTCCCGTTTAGGTGATTTTATCGAATCTGATGGCGAAACCCCAGAAGACCAAGTTTCTAAAAACCTGTTACGGGAA
GATTTAGAAAAAGTCCTCGATAGCCTCAGCCCTCGTGAACGCGATGTTCTCCGTCTACGCTACGGTTTAGATGACGGGCG
GATGAAGACCTTAGAGGAAATTGGACAAATCTTCAATGTCACCCGCGAACGGATTCGGCAAATTGAAGCCAAAGCTCTCC
GTAAATTGCGCCACCCCAACCGCAACAGTGTTCTGAAAGAATATATTCGCTAG

Upstream 100 bases:

>100_bases
AACTGAAAACCCTATCTTCGGTTGTTAACTAGCTCATTTTAAAAAGAGCAGTAAAACCATCTTGAGTAATTGATTCTGCA
AGGAGCATGAGGAACGCGGC

Downstream 100 bases:

>100_bases
TCAGTTGTCAGTGTTCGGTTGTTTTTACTATTGACCACTGACAAATAACTTAAAAACCTGGAGGTACAAATGTACTGCCA
GGTTTTTTGTTTATCTTTTA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNQANNVLDSIYQPDLEIMNQPEIELDDLLIEEDEDLLLADDGDIDEFLEPQTDEDDAKSGKAAKSRRRTQSKKKHYTED
SIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLSEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQ
SNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETIS
RIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKNLLRE
DLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Sequences:

>Translated_390_residues
MNQANNVLDSIYQPDLEIMNQPEIELDDLLIEEDEDLLLADDGDIDEFLEPQTDEDDAKSGKAAKSRRRTQSKKKHYTED
SIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLSEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQ
SNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETIS
RIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKNLLRE
DLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
>Mature_390_residues
MNQANNVLDSIYQPDLEIMNQPEIELDDLLIEEDEDLLLADDGDIDEFLEPQTDEDDAKSGKAAKSRRRTQSKKKHYTED
SIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLSEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQ
SNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETIS
RIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKNLLRE
DLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=59.2741935483871, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789098, Length=360, Percent_Identity=35.2777777777778, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=28.3950617283951, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.7029702970297, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPOD_NOSS1 (P26683)

Other databases:

- EMBL:   M60046
- EMBL:   BA000019
- PIR:   A42724
- PIR:   AG2463
- RefSeq:   NP_489303.1
- ProteinModelPortal:   P26683
- SMR:   P26683
- STRING:   P26683
- GeneID:   1108867
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all5263
- eggNOG:   COG0568
- HOGENOM:   HBG745096
- OMA:   SMEMTIE
- ProtClustDB:   PRK07406
- BioCyc:   NSP103690:ALL5263-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02393
- TIGRFAMs:   TIGR02997
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 45643; Mature: 45643

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQANNVLDSIYQPDLEIMNQPEIELDDLLIEEDEDLLLADDGDIDEFLEPQTDEDDAKS
CCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCHHHC
GKAAKSRRRTQSKKKHYTEDSIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQSNLRLVVSIAKKYMNRGLSF
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETIS
HHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
RIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHH
FIESDGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure
MNQANNVLDSIYQPDLEIMNQPEIELDDLLIEEDEDLLLADDGDIDEFLEPQTDEDDAKS
CCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCHHHC
GKAAKSRRRTQSKKKHYTEDSIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQSNLRLVVSIAKKYMNRGLSF
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETIS
HHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
RIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHH
FIESDGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEIGQIFNV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840