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The map label for this gene is ribE [H]

Identifier: 17232750

GI number: 17232750

Start: 6273708

End: 6274367

Strand: Reverse

Name: ribE [H]

Synonym: all5258

Alternate gene names: 17232750

Gene position: 6274367-6273708 (Counterclockwise)

Preceding gene: 17232755

Following gene: 17232747

Centisome position: 97.83

GC content: 45.0

Gene sequence:

>660_bases
GTGTTTACAGGATTAGTACAAGCATTAGGAACAATCAAACCTTTAGGGGGCGATTCTTGGCAGATTACTTGTGTAAGTCA
GTCATTATCAATTATGCAGGATCTGGCTTATGGTGACAGTGTTGCGGTAGATGGTGTTTGCCTAACAGTAGAAGAAATTT
TAAAAGATGGGTTTATCGCCTCAGCTTCTCCCGAAACCCTGCGCCGCACCACTCTAGGAACTGAGCAAACACAACAAGGA
TACGTCAATCTAGAAACTTCCTTAAGAGTTGGTGGCAAAGTCGGCGGTCACTTTGTCATGGGTCATGTAGATGGCGTAGG
TCAATTGCTAGGGTTTGAACAGACAGCCAGTTCTTGGGAAATGACCTTTACTGCACCGGAAGCGATCGCACGTTACATTG
TCCCCAAGGGTAGCATTACTGTCAATGGCATTAGTCTCACTGTAGCTGCCTACGAGCCTGAATTATCTCAGTTTACCGTG
GCAGTTATTCCTCTTACTTACGCTGAGACTAATCTTCGCTATCTTGTCCCTGGCAGCTTGGTAAATTTGGAAGGGGATAT
CTTAGGCAAGTACGTCGAGAAATTTCTCCAACCCGGTCAACGTCATGCCACAGATAAGGATATTACGCCTGCTTTTCTTT
TAGAACATGGGTATTCGTAA

Upstream 100 bases:

>100_bases
GTCAGAAAATATACTCAGAATTTTTGCCATTCTTGTGAGAATTACCAGTGGATTTTCGGCAAAATTAAGTAATAAATAAA
GTTAGTTTTACAAAAAAGCC

Downstream 100 bases:

>100_bases
TTTGTAATTCACAGACTTGCAAGGACTGGGGTATGGGAGTATTAACTATCCCAGTCCCCAGTCCCCAGTCCCCAGTACCT
AATCCCTAATAGCTCGCGCT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MFTGLVQALGTIKPLGGDSWQITCVSQSLSIMQDLAYGDSVAVDGVCLTVEEILKDGFIASASPETLRRTTLGTEQTQQG
YVNLETSLRVGGKVGGHFVMGHVDGVGQLLGFEQTASSWEMTFTAPEAIARYIVPKGSITVNGISLTVAAYEPELSQFTV
AVIPLTYAETNLRYLVPGSLVNLEGDILGKYVEKFLQPGQRHATDKDITPAFLLEHGYS

Sequences:

>Translated_219_residues
MFTGLVQALGTIKPLGGDSWQITCVSQSLSIMQDLAYGDSVAVDGVCLTVEEILKDGFIASASPETLRRTTLGTEQTQQG
YVNLETSLRVGGKVGGHFVMGHVDGVGQLLGFEQTASSWEMTFTAPEAIARYIVPKGSITVNGISLTVAAYEPELSQFTV
AVIPLTYAETNLRYLVPGSLVNLEGDILGKYVEKFLQPGQRHATDKDITPAFLLEHGYS
>Mature_219_residues
MFTGLVQALGTIKPLGGDSWQITCVSQSLSIMQDLAYGDSVAVDGVCLTVEEILKDGFIASASPETLRRTTLGTEQTQQG
YVNLETSLRVGGKVGGHFVMGHVDGVGQLLGFEQTASSWEMTFTAPEAIARYIVPKGSITVNGISLTVAAYEPELSQFTV
AVIPLTYAETNLRYLVPGSLVNLEGDILGKYVEKFLQPGQRHATDKDITPAFLLEHGYS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=190, Percent_Identity=33.1578947368421, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=36.4077669902913, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23519; Mature: 23519

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLVQALGTIKPLGGDSWQITCVSQSLSIMQDLAYGDSVAVDGVCLTVEEILKDGFIA
CCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEECCEEEEHHHHHHCCCCC
SASPETLRRTTLGTEQTQQGYVNLETSLRVGGKVGGHFVMGHVDGVGQLLGFEQTASSWE
CCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHCCHHCCCCEE
MTFTAPEAIARYIVPKGSITVNGISLTVAAYEPELSQFTVAVIPLTYAETNLRYLVPGSL
EEEECHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEEEEEEEEEECCCCEEEECCCE
VNLEGDILGKYVEKFLQPGQRHATDKDITPAFLLEHGYS
EECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MFTGLVQALGTIKPLGGDSWQITCVSQSLSIMQDLAYGDSVAVDGVCLTVEEILKDGFIA
CCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEECCEEEEHHHHHHCCCCC
SASPETLRRTTLGTEQTQQGYVNLETSLRVGGKVGGHFVMGHVDGVGQLLGFEQTASSWE
CCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHCCHHCCCCEE
MTFTAPEAIARYIVPKGSITVNGISLTVAAYEPELSQFTVAVIPLTYAETNLRYLVPGSL
EEEECHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEEEEEEEEEECCCCEEEECCCE
VNLEGDILGKYVEKFLQPGQRHATDKDITPAFLLEHGYS
EECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]