Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 17232534

GI number: 17232534

Start: 6014294

End: 6015187

Strand: Reverse

Name: potB [H]

Synonym: all5042

Alternate gene names: 17232534

Gene position: 6015187-6014294 (Counterclockwise)

Preceding gene: 17232535

Following gene: 17232533

Centisome position: 93.79

GC content: 43.29

Gene sequence:

>894_bases
GTGAATACAGAAATTCGTGATACTCCAGAGGATCTTGCCAAGATTCAGAAGTTAAACCGCCCAAAGTTAAACTTTATCCA
ACCTTTGGTATTGCTAGCACCGTCTGGTATTTGGTTATTACTATTATTAGTACTACCAACACTGATAATTTTCCAGTTGA
GTCTAGTTCCAAACATTAGACCAGGAGATATAGTCAACCCCAGTGGATTGGATAACTATGTCCGCATACTTGATCCTTTA
TATTTGCAAGTAATCCTACGATCGCTCTGGTTGGCGTTAGGTACAATGGCGATCTGTCTAATTTTGGGTTTTCCGGTAGC
GTATTGGATTGCACAAATTGCACCGAAACGCTGGCGCAATCTCTTGGTACTAGGCTTTGTCTTACCTTTGTGGACTTCTT
CTCTACTCCGTTCTTACGCTTGGATAACGATTCTCCGTCCTACTGGTCTTTTGAACAGTCTATTGAGTAGTGTCGGTTTA
CCCACCTTAGAACTACTTAACCGTACACCAGCTGTTTTAATTGGCATGAGTTACAGCTTACTCCCCTACATGGTGTTGAT
TTTATACGCTTCATTAGAAAAGTTAGATCAACGCTTACTAGAAGCAGCAGCAGATTTAGGCGCAAGTCCCATCAAGGCTT
TTTGGAAAGTCACGGTTCCGCAAATTTACCCAGGAATCACTGCTGGCTCTCTCCTTGTGCTGATCACAGCCTTGGGGGAT
TTTATTGACCCAGAATTACTTGGTGGTGCTTCTAGTATGACGGCAGCCAGGCTAGTTTATAACCAATTTCTCGGAGTTAC
CCAGAATTGGGGGTTTGGTTCGGCGTTGAGTATGACCTTAATTTTTGCCGTGAGTGTAGCGATCGCTCTGCTGATTAAAT
TTGGCGATCGCTAG

Upstream 100 bases:

>100_bases
TTGAAGAAGTTTATGAGCGCTACTGGACGCAATTAACTAGTAGTTAGTTATTGGTAATTGGTAATTGGTCAACAGTCAAC
AGTTAATTACTTTTACAATT

Downstream 100 bases:

>100_bases
TTTCCATGCTCAGTAGAGGTTGCCAATTACTGGGTTGTTACGTATTTTTACTTTAACTTTATATGAAATTTATATAAATA
AATTTATTTTTTCAGTAAAA

Product: permease protein of polyamine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNTEIRDTPEDLAKIQKLNRPKLNFIQPLVLLAPSGIWLLLLLVLPTLIIFQLSLVPNIRPGDIVNPSGLDNYVRILDPL
YLQVILRSLWLALGTMAICLILGFPVAYWIAQIAPKRWRNLLVLGFVLPLWTSSLLRSYAWITILRPTGLLNSLLSSVGL
PTLELLNRTPAVLIGMSYSLLPYMVLILYASLEKLDQRLLEAAADLGASPIKAFWKVTVPQIYPGITAGSLLVLITALGD
FIDPELLGGASSMTAARLVYNQFLGVTQNWGFGSALSMTLIFAVSVAIALLIKFGDR

Sequences:

>Translated_297_residues
MNTEIRDTPEDLAKIQKLNRPKLNFIQPLVLLAPSGIWLLLLLVLPTLIIFQLSLVPNIRPGDIVNPSGLDNYVRILDPL
YLQVILRSLWLALGTMAICLILGFPVAYWIAQIAPKRWRNLLVLGFVLPLWTSSLLRSYAWITILRPTGLLNSLLSSVGL
PTLELLNRTPAVLIGMSYSLLPYMVLILYASLEKLDQRLLEAAADLGASPIKAFWKVTVPQIYPGITAGSLLVLITALGD
FIDPELLGGASSMTAARLVYNQFLGVTQNWGFGSALSMTLIFAVSVAIALLIKFGDR
>Mature_297_residues
MNTEIRDTPEDLAKIQKLNRPKLNFIQPLVLLAPSGIWLLLLLVLPTLIIFQLSLVPNIRPGDIVNPSGLDNYVRILDPL
YLQVILRSLWLALGTMAICLILGFPVAYWIAQIAPKRWRNLLVLGFVLPLWTSSLLRSYAWITILRPTGLLNSLLSSVGL
PTLELLNRTPAVLIGMSYSLLPYMVLILYASLEKLDQRLLEAAADLGASPIKAFWKVTVPQIYPGITAGSLLVLITALGD
FIDPELLGGASSMTAARLVYNQFLGVTQNWGFGSALSMTLIFAVSVAIALLIKFGDR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=245, Percent_Identity=41.6326530612245, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787080, Length=232, Percent_Identity=39.6551724137931, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787713, Length=239, Percent_Identity=31.3807531380753, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32674; Mature: 32674

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEIRDTPEDLAKIQKLNRPKLNFIQPLVLLAPSGIWLLLLLVLPTLIIFQLSLVPNIR
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGDIVNPSGLDNYVRILDPLYLQVILRSLWLALGTMAICLILGFPVAYWIAQIAPKRWRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLVLGFVLPLWTSSLLRSYAWITILRPTGLLNSLLSSVGLPTLELLNRTPAVLIGMSYSL
HHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCCHHHHHCCCCHHEECCHHHH
LPYMVLILYASLEKLDQRLLEAAADLGASPIKAFWKVTVPQIYPGITAGSLLVLITALGD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FIDPELLGGASSMTAARLVYNQFLGVTQNWGFGSALSMTLIFAVSVAIALLIKFGDR
HCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTEIRDTPEDLAKIQKLNRPKLNFIQPLVLLAPSGIWLLLLLVLPTLIIFQLSLVPNIR
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGDIVNPSGLDNYVRILDPLYLQVILRSLWLALGTMAICLILGFPVAYWIAQIAPKRWRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLVLGFVLPLWTSSLLRSYAWITILRPTGLLNSLLSSVGLPTLELLNRTPAVLIGMSYSL
HHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCCHHHHHCCCCHHEECCHHHH
LPYMVLILYASLEKLDQRLLEAAADLGASPIKAFWKVTVPQIYPGITAGSLLVLITALGD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FIDPELLGGASSMTAARLVYNQFLGVTQNWGFGSALSMTLIFAVSVAIALLIKFGDR
HCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]