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The map label for this gene is potD-A [H]

Identifier: 17232535

GI number: 17232535

Start: 6015241

End: 6016323

Strand: Reverse

Name: potD-A [H]

Synonym: all5043

Alternate gene names: 17232535

Gene position: 6016323-6015241 (Counterclockwise)

Preceding gene: 17232536

Following gene: 17232534

Centisome position: 93.8

GC content: 38.41

Gene sequence:

>1083_bases
ATGACTAATAGAAGAAATTTTCTACAAGGAATTACAGCCCTTTCCAGTTTATCCTTAGCAAGTTGTGGTTGGAAATTAGC
TGATGTGCGAGCTTCTAGTTCTTCTGGTCGCAGTGACCAATTGTATCTTTATACTTGGACACAGTATTCTGATCAGAAAC
TATTACAGACTTTTAGCGCCCAAACTGGGATGAAGGCGCTGGTCGATGTCTATGATTCCAACGAAGTAATGCTAGCTAAA
CTCTTAGCTGGAAGTGGTGGTACTTATAGCCTCATTTACCCATCTGACTATATGGTACAGAAGATGGTAGAAAGGGGTTT
GTTAAGTGAATTAAAACACGATCGCCTAGTTGGGTTAGATAATTTATTTCCTCAATTCCAAAATCCTAGTTATGACCCTA
AAAACCGCTATAGCATTCCTTTTAATTGGGGAACAACGGGTTTAATATATAATTCGGAAATTCTCAAAGAAGCACCAGAA
GACTGGGAATATCTATGGCGAAATCAAAAGCTCCTCAGTAAACGTATGACTTTACTCAATGATGTGCGTGAGGTTATAGG
TGCGGTTTTGCGGATGTTGGGTTATTCTTATAACTCGCAAAATGAAGCCCAAATTAAACAAGCTTATGAAAAGTTAAGTA
CACTCAAACCAGCGATCGCAGCTTTTGATACTGATGCTTGGCGCAATCAAATCTTAGCAGGTGATTTGGCATTAGCAATG
TGTTACTCAGCTGATGCTACCAAAGTCATTAAAGAAAATCCTAAATTAAAGTATGTTATTCCCCGTAGTGGTTCTTCACT
ATGGACTGATACAATTGTTATTCCCAAATCAGCGCCCAATTTAGATGGAGCTTATGCCTGGATTAATTTTATTTTACAAC
CAGAAGTAGCAGCTACCACAAGCGAGCGACTGAAGATTTCTACTCCTAATAATGCTGCATTTGAACAATTACCAAAACAA
ATAAAAACTAATGAAAATCTATATCCTCCATCTTCAATTCTAGACAAATGTGAACGCATTAGCCCAGTGGGGCCATTTGA
AGAAGTTTATGAGCGCTACTGGACGCAATTAACTAGTAGTTAG

Upstream 100 bases:

>100_bases
AACCTAATACTTTTGGCAATTTACCAGACCGTGAAACACCTATTTATGCTTGGTGGGCGGAAAGTGATTGTTTGGCTATT
AATCAAATGACTAATGACTA

Downstream 100 bases:

>100_bases
TTATTGGTAATTGGTAATTGGTCAACAGTCAACAGTTAATTACTTTTACAATTGTGAATACAGAAATTCGTGATACTCCA
GAGGATCTTGCCAAGATTCA

Product: polyamine-binding protein of polyamine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTNRRNFLQGITALSSLSLASCGWKLADVRASSSSGRSDQLYLYTWTQYSDQKLLQTFSAQTGMKALVDVYDSNEVMLAK
LLAGSGGTYSLIYPSDYMVQKMVERGLLSELKHDRLVGLDNLFPQFQNPSYDPKNRYSIPFNWGTTGLIYNSEILKEAPE
DWEYLWRNQKLLSKRMTLLNDVREVIGAVLRMLGYSYNSQNEAQIKQAYEKLSTLKPAIAAFDTDAWRNQILAGDLALAM
CYSADATKVIKENPKLKYVIPRSGSSLWTDTIVIPKSAPNLDGAYAWINFILQPEVAATTSERLKISTPNNAAFEQLPKQ
IKTNENLYPPSSILDKCERISPVGPFEEVYERYWTQLTSS

Sequences:

>Translated_360_residues
MTNRRNFLQGITALSSLSLASCGWKLADVRASSSSGRSDQLYLYTWTQYSDQKLLQTFSAQTGMKALVDVYDSNEVMLAK
LLAGSGGTYSLIYPSDYMVQKMVERGLLSELKHDRLVGLDNLFPQFQNPSYDPKNRYSIPFNWGTTGLIYNSEILKEAPE
DWEYLWRNQKLLSKRMTLLNDVREVIGAVLRMLGYSYNSQNEAQIKQAYEKLSTLKPAIAAFDTDAWRNQILAGDLALAM
CYSADATKVIKENPKLKYVIPRSGSSLWTDTIVIPKSAPNLDGAYAWINFILQPEVAATTSERLKISTPNNAAFEQLPKQ
IKTNENLYPPSSILDKCERISPVGPFEEVYERYWTQLTSS
>Mature_359_residues
TNRRNFLQGITALSSLSLASCGWKLADVRASSSSGRSDQLYLYTWTQYSDQKLLQTFSAQTGMKALVDVYDSNEVMLAKL
LAGSGGTYSLIYPSDYMVQKMVERGLLSELKHDRLVGLDNLFPQFQNPSYDPKNRYSIPFNWGTTGLIYNSEILKEAPED
WEYLWRNQKLLSKRMTLLNDVREVIGAVLRMLGYSYNSQNEAQIKQAYEKLSTLKPAIAAFDTDAWRNQILAGDLALAMC
YSADATKVIKENPKLKYVIPRSGSSLWTDTIVIPKSAPNLDGAYAWINFILQPEVAATTSERLKISTPNNAAFEQLPKQI
KTNENLYPPSSILDKCERISPVGPFEEVYERYWTQLTSS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=331, Percent_Identity=33.5347432024169, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787078, Length=354, Percent_Identity=28.8135593220339, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40692; Mature: 40560

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRRNFLQGITALSSLSLASCGWKLADVRASSSSGRSDQLYLYTWTQYSDQKLLQTFSA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHH
QTGMKALVDVYDSNEVMLAKLLAGSGGTYSLIYPSDYMVQKMVERGLLSELKHDRLVGLD
HCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
NLFPQFQNPSYDPKNRYSIPFNWGTTGLIYNSEILKEAPEDWEYLWRNQKLLSKRMTLLN
HHCCCCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DVREVIGAVLRMLGYSYNSQNEAQIKQAYEKLSTLKPAIAAFDTDAWRNQILAGDLALAM
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
CYSADATKVIKENPKLKYVIPRSGSSLWTDTIVIPKSAPNLDGAYAWINFILQPEVAATT
HHCCCHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHCC
SERLKISTPNNAAFEQLPKQIKTNENLYPPSSILDKCERISPVGPFEEVYERYWTQLTSS
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNRRNFLQGITALSSLSLASCGWKLADVRASSSSGRSDQLYLYTWTQYSDQKLLQTFSA
CCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHH
QTGMKALVDVYDSNEVMLAKLLAGSGGTYSLIYPSDYMVQKMVERGLLSELKHDRLVGLD
HCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
NLFPQFQNPSYDPKNRYSIPFNWGTTGLIYNSEILKEAPEDWEYLWRNQKLLSKRMTLLN
HHCCCCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DVREVIGAVLRMLGYSYNSQNEAQIKQAYEKLSTLKPAIAAFDTDAWRNQILAGDLALAM
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
CYSADATKVIKENPKLKYVIPRSGSSLWTDTIVIPKSAPNLDGAYAWINFILQPEVAATT
HHCCCHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHCC
SERLKISTPNNAAFEQLPKQIKTNENLYPPSSILDKCERISPVGPFEEVYERYWTQLTSS
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]